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Article: Co-expression of a prophage system and a plasmid system in Bacillus subtilis

TitleCo-expression of a prophage system and a plasmid system in Bacillus subtilis
Authors
KeywordsAmylase
Bacillus subtilis
Dual expression
Lipase
Plasmid
Prophage
Protease
Issue Date2003
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yprep
Citation
Protein Expression And Purification, 2003, v. 32 n. 2, p. 293-301 How to Cite?
AbstractA dual expression system for overexpressing two proteins by a single cell strain has been developed in Bacillus subtilis. This dual expression system combines the Φ105MU331 prophage system and a plasmid system within a single cell. Protein expression by the prophage system is heat inducible, while that of the plasmid system is constitutive. Three candidate genes, BPN, BT, and amyE, all of Bacillus origin, were used as test models. Seven strains (BPN, BT, AMY, BS168K, MU331K, BPNK, and BTK) were constructed to investigate the influences of the prophage system and the plasmid system on each other, and to compare the efficiency of the individual expression systems with that of the dual expression system. Individually, the yield of the plasmid system is higher than that of the prophage system, which could be attributed to the constitutive nature of the expression of the plasmid system. Nonetheless, for the dual expression strains, the expression of two enzymes in a single fermentation run can reduce costs in facilities, manpower, and utilities. Fed-batch fermentation of BPNK strains confirmed the feasibility of applying this dual expression system in industrial-scale production. © 2003 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/54338
ISSN
2015 Impact Factor: 1.407
2015 SCImago Journal Rankings: 0.767
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHo, KMen_HK
dc.contributor.authorLim, BLen_HK
dc.date.accessioned2009-04-03T07:43:47Z-
dc.date.available2009-04-03T07:43:47Z-
dc.date.issued2003en_HK
dc.identifier.citationProtein Expression And Purification, 2003, v. 32 n. 2, p. 293-301en_HK
dc.identifier.issn1046-5928en_HK
dc.identifier.urihttp://hdl.handle.net/10722/54338-
dc.description.abstractA dual expression system for overexpressing two proteins by a single cell strain has been developed in Bacillus subtilis. This dual expression system combines the Φ105MU331 prophage system and a plasmid system within a single cell. Protein expression by the prophage system is heat inducible, while that of the plasmid system is constitutive. Three candidate genes, BPN, BT, and amyE, all of Bacillus origin, were used as test models. Seven strains (BPN, BT, AMY, BS168K, MU331K, BPNK, and BTK) were constructed to investigate the influences of the prophage system and the plasmid system on each other, and to compare the efficiency of the individual expression systems with that of the dual expression system. Individually, the yield of the plasmid system is higher than that of the prophage system, which could be attributed to the constitutive nature of the expression of the plasmid system. Nonetheless, for the dual expression strains, the expression of two enzymes in a single fermentation run can reduce costs in facilities, manpower, and utilities. Fed-batch fermentation of BPNK strains confirmed the feasibility of applying this dual expression system in industrial-scale production. © 2003 Elsevier Inc. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yprepen_HK
dc.relation.ispartofProtein Expression and Purificationen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectAmylaseen_HK
dc.subjectBacillus subtilisen_HK
dc.subjectDual expressionen_HK
dc.subjectLipaseen_HK
dc.subjectPlasmiden_HK
dc.subjectProphageen_HK
dc.subjectProteaseen_HK
dc.subject.meshBacillus subtilis - genetics - metabolismen_HK
dc.subject.meshBioreactorsen_HK
dc.subject.meshDNA Primers - geneticsen_HK
dc.subject.meshEndopeptidases - genetics - metabolismen_HK
dc.subject.meshPlasmids - geneticsen_HK
dc.titleCo-expression of a prophage system and a plasmid system in Bacillus subtilisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1046-5928&volume=32&issue=2&spage=293&epage=301&date=2003&atitle=Co-expression+of+a+prophage+system+and+a+plasmid+system+in+Bacillus+subtilisen_HK
dc.identifier.emailLim, BL: bllim@hkucc.hku.hken_HK
dc.identifier.authorityLim, BL=rp00744en_HK
dc.description.naturepostprinten_HK
dc.identifier.doi10.1016/j.pep.2003.08.002en_HK
dc.identifier.pmid14965776-
dc.identifier.scopuseid_2-s2.0-0347567025en_HK
dc.identifier.hkuros85498-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0347567025&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume32en_HK
dc.identifier.issue2en_HK
dc.identifier.spage293en_HK
dc.identifier.epage301en_HK
dc.identifier.isiWOS:000187393600016-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridHo, KM=7403581729en_HK
dc.identifier.scopusauthoridLim, BL=7201983917en_HK

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