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Article: A dual protein expression system in Bacillus subtilis

TitleA dual protein expression system in Bacillus subtilis
Authors
KeywordsBacillus subtilis
Dual expression
Glucanase
Prophage
Xylanase
Issue Date2002
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yprep
Citation
Protein Expression And Purification, 2002, v. 26 n. 3, p. 337-342 How to Cite?
AbstractWe have developed a dual expression system for the simultaneous overexpression of two proteins in Bacillus subtilis. Two candidate genes, xylanase (xynA) and glucanase (bglS) from B. subtilis strain 168, which were engineered with independent Shine-Dalgarno (SD) sequences, were cloned in tandem into a transfer vector, which was then transformed into B. subtilis strain 1A304 (φ105MU331). The genes were under the transcriptional control of a strong promoter of a bacteriophage, φ105, where transcription was initiated upon thermal induction. Six constructs were made to compare the factors that affected the yields of the gene products. The expression level of each candidate gene was found to correspond to its position relative to the phage promoter, irrespective of the identity of the insert. The lower expression level of the second insert might have been due to limited resources for protein synthesis, a short half-life of the mRNA, or an early termination of the RNA polymerase. Curiously, gene duplications in tandem did not lead to further increase in production. © 2002 Elsevier Science (USA). All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/54337
ISSN
2015 Impact Factor: 1.407
2015 SCImago Journal Rankings: 0.767
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChan, AYen_HK
dc.contributor.authorChan, MMen_HK
dc.contributor.authorLo, HMen_HK
dc.contributor.authorLeung, YCen_HK
dc.contributor.authorLim, BLen_HK
dc.date.accessioned2009-04-03T07:43:45Z-
dc.date.available2009-04-03T07:43:45Z-
dc.date.issued2002en_HK
dc.identifier.citationProtein Expression And Purification, 2002, v. 26 n. 3, p. 337-342en_HK
dc.identifier.issn1046-5928en_HK
dc.identifier.urihttp://hdl.handle.net/10722/54337-
dc.description.abstractWe have developed a dual expression system for the simultaneous overexpression of two proteins in Bacillus subtilis. Two candidate genes, xylanase (xynA) and glucanase (bglS) from B. subtilis strain 168, which were engineered with independent Shine-Dalgarno (SD) sequences, were cloned in tandem into a transfer vector, which was then transformed into B. subtilis strain 1A304 (φ105MU331). The genes were under the transcriptional control of a strong promoter of a bacteriophage, φ105, where transcription was initiated upon thermal induction. Six constructs were made to compare the factors that affected the yields of the gene products. The expression level of each candidate gene was found to correspond to its position relative to the phage promoter, irrespective of the identity of the insert. The lower expression level of the second insert might have been due to limited resources for protein synthesis, a short half-life of the mRNA, or an early termination of the RNA polymerase. Curiously, gene duplications in tandem did not lead to further increase in production. © 2002 Elsevier Science (USA). All rights reserved.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yprepen_HK
dc.relation.ispartofProtein Expression and Purificationen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectBacillus subtilisen_HK
dc.subjectDual expressionen_HK
dc.subjectGlucanaseen_HK
dc.subjectProphageen_HK
dc.subjectXylanaseen_HK
dc.subject.meshBacillus subtilis - geneticsen_HK
dc.subject.meshGene Expressionen_HK
dc.subject.meshGenes, Bacterial - geneticsen_HK
dc.subject.meshGenetic Engineering - methodsen_HK
dc.subject.meshTranscription, Geneticen_HK
dc.titleA dual protein expression system in Bacillus subtilisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1046-5928&volume=26&issue=3&spage=337&epage=342&date=2002&atitle=A+dual+protein+expression+system+in+Bacillus+subtilisen_HK
dc.identifier.emailLim, BL: bllim@hkucc.hku.hken_HK
dc.identifier.authorityLim, BL=rp00744en_HK
dc.description.naturepostprinten_HK
dc.identifier.doi10.1016/S1046-5928(02)00552-1en_HK
dc.identifier.pmid12460756en_HK
dc.identifier.scopuseid_2-s2.0-0036915133en_HK
dc.identifier.hkuros75821-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036915133&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume26en_HK
dc.identifier.issue3en_HK
dc.identifier.spage337en_HK
dc.identifier.epage342en_HK
dc.identifier.isiWOS:000179820900001-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChan, AY=26021082600en_HK
dc.identifier.scopusauthoridChan, MM=36941307600en_HK
dc.identifier.scopusauthoridLo, HM=7202085429en_HK
dc.identifier.scopusauthoridLeung, YC=35074432700en_HK
dc.identifier.scopusauthoridLim, BL=7201983917en_HK
dc.identifier.citeulike15822-

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