Article: The introduction of a phytase gene from Bacillus subtilis improved the growth performance of transgenic tobacco

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TitleThe introduction of a phytase gene from Bacillus subtilis improved the growth performance of transgenic tobacco
AuthorsYip, W1
Wang, L1 2
Cheng, C1
Wu, W1
Lung, S1
Lim, BL1
KeywordsBacillus
Inositol phosphate
Phosphorus
Phytase
Phytic acid
Issue Date2003
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description
CitationBiochemical And Biophysical Research Communications, 2003, v. 310 n. 4, p. 1148-1154 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.bbrc.2003.09.136
AbstractPhytate, the main form of phosphorus storage in plant seeds, is well known to be an anti-nutrient and a major source of phosphorus pollution in animal manure. To improve phosphorus bio-availability, we introduced a recently characterized phytase from Bacillus subtilis into the cytoplasm of tobacco cells. Although the introduction of acid fungal phytase from Aspergillus niger in previous studies did not result in any phenotypic changes in tobacco, here we show that a tobacco line transformed with a neutral phytase exhibited phenotypic changes in flowering, seed development, and response to phosphate deficiency. The transgenic line showed an increase in flower and fruit numbers, small seed syndrome, lower seed IP6/IP5 ratio, and enhanced growth under phosphate-starvation conditions compared with the wildtype. The results suggest that the over-expression of Bacillus phytase in the cytoplasm of tobacco cells shifts the equilibrium of the inositol phosphate biosynthesis pathway, thereby making more phosphate available for primary metabolism. The approach presented here can be applied as a strategy for boosting productivity in agriculture and horticulture. © 2003 Elsevier Inc. All rights reserved.
ISSN0006-291X
2011 Impact Factor: 2.484
2011 SCImago Journal Rankings: 0.287
DOIhttp://dx.doi.org/10.1016/j.bbrc.2003.09.136
ISI Accession Number IDWOS:000186146200017
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorYip, W
dc.contributor.authorWang, L
dc.contributor.authorCheng, C
dc.contributor.authorWu, W
dc.contributor.authorLung, S
dc.contributor.authorLim, BL
dc.date.accessioned2009-04-03T07:41:02Z
dc.date.available2009-04-03T07:41:02Z
dc.date.issued2003
dc.description.abstractPhytate, the main form of phosphorus storage in plant seeds, is well known to be an anti-nutrient and a major source of phosphorus pollution in animal manure. To improve phosphorus bio-availability, we introduced a recently characterized phytase from Bacillus subtilis into the cytoplasm of tobacco cells. Although the introduction of acid fungal phytase from Aspergillus niger in previous studies did not result in any phenotypic changes in tobacco, here we show that a tobacco line transformed with a neutral phytase exhibited phenotypic changes in flowering, seed development, and response to phosphate deficiency. The transgenic line showed an increase in flower and fruit numbers, small seed syndrome, lower seed IP6/IP5 ratio, and enhanced growth under phosphate-starvation conditions compared with the wildtype. The results suggest that the over-expression of Bacillus phytase in the cytoplasm of tobacco cells shifts the equilibrium of the inositol phosphate biosynthesis pathway, thereby making more phosphate available for primary metabolism. The approach presented here can be applied as a strategy for boosting productivity in agriculture and horticulture. © 2003 Elsevier Inc. All rights reserved.
dc.description.naturepostprint
dc.identifier.citationBiochemical And Biophysical Research Communications, 2003, v. 310 n. 4, p. 1148-1154 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.bbrc.2003.09.136
dc.identifier.doihttp://dx.doi.org/10.1016/j.bbrc.2003.09.136
dc.identifier.epage1154
dc.identifier.hkuros85762
dc.identifier.isiWOS:000186146200017
dc.identifier.issn0006-291X
2011 Impact Factor: 2.484
2011 SCImago Journal Rankings: 0.287
dc.identifier.issue4
dc.identifier.openurl
dc.identifier.pmid14559235
dc.identifier.scopuseid_2-s2.0-0141892638
dc.identifier.spage1148
dc.identifier.urihttp://hdl.handle.net/10722/54247
dc.identifier.volume310
dc.languageeng
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description
dc.publisher.placeUnited States
dc.relation.ispartofBiochemical and Biophysical Research Communications
dc.relation.referencesReferences in Scopus
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
dc.subject.mesh6-Phytase - genetics
dc.subject.meshBacillus subtilis - enzymology - genetics
dc.subject.meshChromatography, High Pressure Liquid
dc.subject.meshTobacco/genetics - growth & development
dc.subject.meshPlants, Genetically Modified/genetics - growth & development
dc.subjectBacillus
dc.subjectInositol phosphate
dc.subjectPhosphorus
dc.subjectPhytase
dc.subjectPhytic acid
dc.titleThe introduction of a phytase gene from Bacillus subtilis improved the growth performance of transgenic tobacco
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong
  2. Institute of Plant Physiology and Ecology, Shanghai Institute for Biological Sciences Chinese Academy of Sciences