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Article: The introduction of a phytase gene from Bacillus subtilis improved the growth performance of transgenic tobacco

TitleThe introduction of a phytase gene from Bacillus subtilis improved the growth performance of transgenic tobacco
Authors
KeywordsBacillus
Inositol phosphate
Phosphorus
Phytase
Phytic acid
Issue Date2003
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description
Citation
Biochemical And Biophysical Research Communications, 2003, v. 310 n. 4, p. 1148-1154 How to Cite?
Abstract
Phytate, the main form of phosphorus storage in plant seeds, is well known to be an anti-nutrient and a major source of phosphorus pollution in animal manure. To improve phosphorus bio-availability, we introduced a recently characterized phytase from Bacillus subtilis into the cytoplasm of tobacco cells. Although the introduction of acid fungal phytase from Aspergillus niger in previous studies did not result in any phenotypic changes in tobacco, here we show that a tobacco line transformed with a neutral phytase exhibited phenotypic changes in flowering, seed development, and response to phosphate deficiency. The transgenic line showed an increase in flower and fruit numbers, small seed syndrome, lower seed IP6/IP5 ratio, and enhanced growth under phosphate-starvation conditions compared with the wildtype. The results suggest that the over-expression of Bacillus phytase in the cytoplasm of tobacco cells shifts the equilibrium of the inositol phosphate biosynthesis pathway, thereby making more phosphate available for primary metabolism. The approach presented here can be applied as a strategy for boosting productivity in agriculture and horticulture. © 2003 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/54247
ISSN
2013 Impact Factor: 2.281
ISI Accession Number ID
References

 

Author Affiliations
  1. The University of Hong Kong
  2. Institute of Plant Physiology and Ecology, Shanghai Institute for Biological Sciences Chinese Academy of Sciences
DC FieldValueLanguage
dc.contributor.authorYip, Wen_HK
dc.contributor.authorWang, Len_HK
dc.contributor.authorCheng, Cen_HK
dc.contributor.authorWu, Wen_HK
dc.contributor.authorLung, Sen_HK
dc.contributor.authorLim, BLen_HK
dc.date.accessioned2009-04-03T07:41:02Z-
dc.date.available2009-04-03T07:41:02Z-
dc.date.issued2003en_HK
dc.identifier.citationBiochemical And Biophysical Research Communications, 2003, v. 310 n. 4, p. 1148-1154en_HK
dc.identifier.issn0006-291Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/54247-
dc.description.abstractPhytate, the main form of phosphorus storage in plant seeds, is well known to be an anti-nutrient and a major source of phosphorus pollution in animal manure. To improve phosphorus bio-availability, we introduced a recently characterized phytase from Bacillus subtilis into the cytoplasm of tobacco cells. Although the introduction of acid fungal phytase from Aspergillus niger in previous studies did not result in any phenotypic changes in tobacco, here we show that a tobacco line transformed with a neutral phytase exhibited phenotypic changes in flowering, seed development, and response to phosphate deficiency. The transgenic line showed an increase in flower and fruit numbers, small seed syndrome, lower seed IP6/IP5 ratio, and enhanced growth under phosphate-starvation conditions compared with the wildtype. The results suggest that the over-expression of Bacillus phytase in the cytoplasm of tobacco cells shifts the equilibrium of the inositol phosphate biosynthesis pathway, thereby making more phosphate available for primary metabolism. The approach presented here can be applied as a strategy for boosting productivity in agriculture and horticulture. © 2003 Elsevier Inc. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/descriptionen_HK
dc.relation.ispartofBiochemical and Biophysical Research Communicationsen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectBacillusen_HK
dc.subjectInositol phosphateen_HK
dc.subjectPhosphorusen_HK
dc.subjectPhytaseen_HK
dc.subjectPhytic aciden_HK
dc.subject.mesh6-Phytase - geneticsen_HK
dc.subject.meshBacillus subtilis - enzymology - geneticsen_HK
dc.subject.meshChromatography, High Pressure Liquiden_HK
dc.subject.meshTobacco/genetics - growth & developmenten_HK
dc.subject.meshPlants, Genetically Modified/genetics - growth & developmenten_HK
dc.titleThe introduction of a phytase gene from Bacillus subtilis improved the growth performance of transgenic tobaccoen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-291X&volume=310&issue=4&spage=1148&epage=1154&date=2003&atitle=The+introduction+of+a+phytase+gene+from+Bacillus+subtilis+improved+the+growth+performance+of+transgenic+tobaccoen_HK
dc.identifier.emailYip, W: wkyip@hkucc.hku.hken_HK
dc.identifier.emailLim, BL: bllim@hkucc.hku.hken_HK
dc.identifier.authorityYip, W=rp00833en_HK
dc.identifier.authorityLim, BL=rp00744en_HK
dc.description.naturepostprinten_HK
dc.identifier.doi10.1016/j.bbrc.2003.09.136en_HK
dc.identifier.pmid14559235en_HK
dc.identifier.scopuseid_2-s2.0-0141892638en_HK
dc.identifier.hkuros85762-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0141892638&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume310en_HK
dc.identifier.issue4en_HK
dc.identifier.spage1148en_HK
dc.identifier.epage1154en_HK
dc.identifier.isiWOS:000186146200017-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridYip, W=7102784428en_HK
dc.identifier.scopusauthoridWang, L=7409175686en_HK
dc.identifier.scopusauthoridCheng, C=7404797223en_HK
dc.identifier.scopusauthoridWu, W=16835478800en_HK
dc.identifier.scopusauthoridLung, S=8619522500en_HK
dc.identifier.scopusauthoridLim, BL=7201983917en_HK

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