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Article: The introduction of a phytase gene from Bacillus subtilis improved the growth performance of transgenic tobacco
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TitleThe introduction of a phytase gene from Bacillus subtilis improved the growth performance of transgenic tobacco
 
AuthorsYip, W1
Wang, L1 2
Cheng, C1
Wu, W1
Lung, S1
Lim, BL1
 
KeywordsBacillus
Inositol phosphate
Phosphorus
Phytase
Phytic acid
 
Issue Date2003
 
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description
 
CitationBiochemical And Biophysical Research Communications, 2003, v. 310 n. 4, p. 1148-1154 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.bbrc.2003.09.136
 
AbstractPhytate, the main form of phosphorus storage in plant seeds, is well known to be an anti-nutrient and a major source of phosphorus pollution in animal manure. To improve phosphorus bio-availability, we introduced a recently characterized phytase from Bacillus subtilis into the cytoplasm of tobacco cells. Although the introduction of acid fungal phytase from Aspergillus niger in previous studies did not result in any phenotypic changes in tobacco, here we show that a tobacco line transformed with a neutral phytase exhibited phenotypic changes in flowering, seed development, and response to phosphate deficiency. The transgenic line showed an increase in flower and fruit numbers, small seed syndrome, lower seed IP6/IP5 ratio, and enhanced growth under phosphate-starvation conditions compared with the wildtype. The results suggest that the over-expression of Bacillus phytase in the cytoplasm of tobacco cells shifts the equilibrium of the inositol phosphate biosynthesis pathway, thereby making more phosphate available for primary metabolism. The approach presented here can be applied as a strategy for boosting productivity in agriculture and horticulture. © 2003 Elsevier Inc. All rights reserved.
 
ISSN0006-291X
2013 Impact Factor: 2.281
 
DOIhttp://dx.doi.org/10.1016/j.bbrc.2003.09.136
 
ISI Accession Number IDWOS:000186146200017
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorYip, W
 
dc.contributor.authorWang, L
 
dc.contributor.authorCheng, C
 
dc.contributor.authorWu, W
 
dc.contributor.authorLung, S
 
dc.contributor.authorLim, BL
 
dc.date.accessioned2009-04-03T07:41:02Z
 
dc.date.available2009-04-03T07:41:02Z
 
dc.date.issued2003
 
dc.description.abstractPhytate, the main form of phosphorus storage in plant seeds, is well known to be an anti-nutrient and a major source of phosphorus pollution in animal manure. To improve phosphorus bio-availability, we introduced a recently characterized phytase from Bacillus subtilis into the cytoplasm of tobacco cells. Although the introduction of acid fungal phytase from Aspergillus niger in previous studies did not result in any phenotypic changes in tobacco, here we show that a tobacco line transformed with a neutral phytase exhibited phenotypic changes in flowering, seed development, and response to phosphate deficiency. The transgenic line showed an increase in flower and fruit numbers, small seed syndrome, lower seed IP6/IP5 ratio, and enhanced growth under phosphate-starvation conditions compared with the wildtype. The results suggest that the over-expression of Bacillus phytase in the cytoplasm of tobacco cells shifts the equilibrium of the inositol phosphate biosynthesis pathway, thereby making more phosphate available for primary metabolism. The approach presented here can be applied as a strategy for boosting productivity in agriculture and horticulture. © 2003 Elsevier Inc. All rights reserved.
 
dc.description.naturepostprint
 
dc.identifier.citationBiochemical And Biophysical Research Communications, 2003, v. 310 n. 4, p. 1148-1154 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.bbrc.2003.09.136
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.bbrc.2003.09.136
 
dc.identifier.epage1154
 
dc.identifier.hkuros85762
 
dc.identifier.isiWOS:000186146200017
 
dc.identifier.issn0006-291X
2013 Impact Factor: 2.281
 
dc.identifier.issue4
 
dc.identifier.openurl
 
dc.identifier.pmid14559235
 
dc.identifier.scopuseid_2-s2.0-0141892638
 
dc.identifier.spage1148
 
dc.identifier.urihttp://hdl.handle.net/10722/54247
 
dc.identifier.volume310
 
dc.languageeng
 
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description
 
dc.publisher.placeUnited States
 
dc.relation.ispartofBiochemical and Biophysical Research Communications
 
dc.relation.referencesReferences in Scopus
 
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
 
dc.subject.mesh6-Phytase - genetics
 
dc.subject.meshBacillus subtilis - enzymology - genetics
 
dc.subject.meshChromatography, High Pressure Liquid
 
dc.subject.meshTobacco/genetics - growth & development
 
dc.subject.meshPlants, Genetically Modified/genetics - growth & development
 
dc.subjectBacillus
 
dc.subjectInositol phosphate
 
dc.subjectPhosphorus
 
dc.subjectPhytase
 
dc.subjectPhytic acid
 
dc.titleThe introduction of a phytase gene from Bacillus subtilis improved the growth performance of transgenic tobacco
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong
  2. Institute of Plant Physiology and Ecology, Shanghai Institute for Biological Sciences Chinese Academy of Sciences