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Article: Localization and variable expression of Gαi2 in human endometrium and Fallopian tubes

TitleLocalization and variable expression of Gαi2 in human endometrium and Fallopian tubes
Authors
KeywordsCilia
Endometrium
Fallopian tube
G protein
Menstrual cycle
Issue Date2007
PublisherOxford University Press. The Journal's web site is located at http://humrep.oxfordjournals.org/
Citation
Human Reproduction, 2007, v. 22 n. 5, p. 1224-1230 How to Cite?
AbstractBackground: Heterotrimeric G proteins take part in membrane-mediated cell signalling and have a role in hormonal regulation. This study clarifies the expression and localization of the G protein subunit Gαi2 in the human endometrium and Fallopian tube and changes in Gαi2 expression in human endometrium during the menstrual cycle. Methods: The expression of Gαi2 was identified by Polymerase chain reaction (PCR), and localization confirmed by immunostaining. Cyclic changes in Gαi2 expression during the menstrual cycle were evaluated by quantitative real-time PCR. Results: We found Gαi2 to be expressed in human endometrium, Fallopian tube tissue and in primary cultures of Fallopian tube epithelial cells. Our studies revealed enriched localization of Gαi2 in Fallopian tube cilia and in endometrial glands. We showed that Gαi2 expression in human endometrium changes significantly during the menstrual cycle, with a higher level in the secretory versus proliferative and menstrual phases (P < 0.05). Conclusions: Gαi2 is specifically localized in human Fallopian tube epithelial cells, particularly in the cilia, and is likely to have a cilia-specific role in reproduction. Significantly variable expression of Gαi2 during the menstrual cycle suggests Gαi2 might be under hormonal regulation in the female reproductive tract in vivo. © 2007 Oxford University Press.
Persistent Identifierhttp://hdl.handle.net/10722/54238
ISSN
2015 Impact Factor: 4.621
2015 SCImago Journal Rankings: 2.271
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorMönkkönen, KSen_HK
dc.contributor.authorAflatoonian, Ren_HK
dc.contributor.authorLee, KFen_HK
dc.contributor.authorYeung, WSBen_HK
dc.contributor.authorTsao, SWen_HK
dc.contributor.authorLaitinen, JTen_HK
dc.contributor.authorTuckerman, EMen_HK
dc.contributor.authorLi, TCen_HK
dc.contributor.authorFazeli, Aen_HK
dc.date.accessioned2009-04-03T07:40:44Z-
dc.date.available2009-04-03T07:40:44Z-
dc.date.issued2007en_HK
dc.identifier.citationHuman Reproduction, 2007, v. 22 n. 5, p. 1224-1230en_HK
dc.identifier.issn0268-1161en_HK
dc.identifier.urihttp://hdl.handle.net/10722/54238-
dc.description.abstractBackground: Heterotrimeric G proteins take part in membrane-mediated cell signalling and have a role in hormonal regulation. This study clarifies the expression and localization of the G protein subunit Gαi2 in the human endometrium and Fallopian tube and changes in Gαi2 expression in human endometrium during the menstrual cycle. Methods: The expression of Gαi2 was identified by Polymerase chain reaction (PCR), and localization confirmed by immunostaining. Cyclic changes in Gαi2 expression during the menstrual cycle were evaluated by quantitative real-time PCR. Results: We found Gαi2 to be expressed in human endometrium, Fallopian tube tissue and in primary cultures of Fallopian tube epithelial cells. Our studies revealed enriched localization of Gαi2 in Fallopian tube cilia and in endometrial glands. We showed that Gαi2 expression in human endometrium changes significantly during the menstrual cycle, with a higher level in the secretory versus proliferative and menstrual phases (P < 0.05). Conclusions: Gαi2 is specifically localized in human Fallopian tube epithelial cells, particularly in the cilia, and is likely to have a cilia-specific role in reproduction. Significantly variable expression of Gαi2 during the menstrual cycle suggests Gαi2 might be under hormonal regulation in the female reproductive tract in vivo. © 2007 Oxford University Press.en_HK
dc.languageengen_HK
dc.publisherOxford University Press. The Journal's web site is located at http://humrep.oxfordjournals.org/en_HK
dc.relation.ispartofHuman Reproductionen_HK
dc.rightsHuman Reproduction. Copyright © Oxford University Press.en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsThis is a pre-copy-editing, author-produced PDF of an article accepted for publication in Human Reproduction following peer review. The definitive publisher-authenticated version Human Reproduction, 2007, v. 22 n. 5, p. 1224-1230 is available online at: http://humrep.oxfordjournals.org/en_HK
dc.subjectCiliaen_HK
dc.subjectEndometriumen_HK
dc.subjectFallopian tubeen_HK
dc.subjectG proteinen_HK
dc.subjectMenstrual cycleen_HK
dc.subject.meshEndometrium - metabolismen_HK
dc.subject.meshFallopian Tubes - metabolismen_HK
dc.subject.meshP-Binding Protein alpha Subunit, Gi2 - biosynthesisen_HK
dc.subject.meshGene Expression Regulationen_HK
dc.subject.meshImmunohistochemistryen_HK
dc.titleLocalization and variable expression of Gαi2 in human endometrium and Fallopian tubesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0268-1161&volume=22&issue=5&spage=1224&epage=1230&date=2007&atitle=Localization+and+variable+expression+of+Galphai2+in+human+endometrium+and+fallopian+tubes.en_HK
dc.identifier.emailLee, KF:ckflee@hku.hken_HK
dc.identifier.emailYeung, WSB:wsbyeung@hkucc.hku.hken_HK
dc.identifier.emailTsao, SW:gswtsao@hkucc.hku.hken_HK
dc.identifier.authorityLee, KF=rp00458en_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.identifier.authorityTsao, SW=rp00399en_HK
dc.description.naturepostprinten_HK
dc.identifier.doi10.1093/humrep/dem022en_HK
dc.identifier.pmid17347170-
dc.identifier.scopuseid_2-s2.0-34447101069en_HK
dc.identifier.hkuros127481-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34447101069&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume22en_HK
dc.identifier.issue5en_HK
dc.identifier.spage1224en_HK
dc.identifier.epage1230en_HK
dc.identifier.isiWOS:000246802500005-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridMönkkönen, KS=8901685600en_HK
dc.identifier.scopusauthoridAflatoonian, R=16744883000en_HK
dc.identifier.scopusauthoridLee, KF=26643097500en_HK
dc.identifier.scopusauthoridYeung, WSB=7102370745en_HK
dc.identifier.scopusauthoridTsao, SW=7102813116en_HK
dc.identifier.scopusauthoridLaitinen, JT=7103329968en_HK
dc.identifier.scopusauthoridTuckerman, EM=15833600700en_HK
dc.identifier.scopusauthoridLi, TC=24302527400en_HK
dc.identifier.scopusauthoridFazeli, A=7003548599en_HK
dc.identifier.citeulike1392080-

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