File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Mutational analysis of the PTEN/MMAC1 gene in primary oesophageal squamous cell carcinomas

TitleMutational analysis of the PTEN/MMAC1 gene in primary oesophageal squamous cell carcinomas
Authors
KeywordsMutation
Oesophageal carcinoma
Polymerase chain reaction single strand conformational polymorphism analysis
PTEN/MMAC1
Issue Date1999
PublisherBMJ Publishing Group. The Journal's web site is located at http://mp.bmjjournals.com/
Citation
Journal of Clinical Pathology - Molecular Pathology, 1999, v. 52 n. 6, p. 353-356 How to Cite?
AbstractAIM: To investigate whether PTEN/MMAC1 mutations play a role in the carcinogenesis of oesophageal squamous cell carcinoma. METHODS: A panel of 33 primary oesophageal squamous cell carcinoma tumour samples and 20 corresponding morphologically normal tissues was examined for mutations in all nine exons of the PTEN/MMAC1 gene by means of polymerase chain reaction single strand conformational polymorphism analysis (PCR-SSCP) and direct DNA sequencing methods. RESULTS: Only one of 33 oesophageal squamous cell carcinomas showed an aberrant SSCP band. Further sequencing analysis of this sample revealed an 802 -29 T → C substitution in intron 7. PTEN/MMAC1 mutations were not found in the mutational 'hot spot' in exon 5, even after direct sequencing of six oesophageal squamous cell carcinoma samples and three normal tissues. However, a deletion of one nucleotide T at position 492 +8 in intron 5 was seen in all samples. CONCLUSION: These results suggest that PTEN/MMAC1 mutations do not play a major role in the carcinogenesis of oesophageal squamous cell carcinoma.
Persistent Identifierhttp://hdl.handle.net/10722/54184
ISSN
2005 Impact Factor: 2.576
2005 SCImago Journal Rankings: 0.122
PubMed Central ID
References

 

DC FieldValueLanguage
dc.contributor.authorHu, YCen_HK
dc.contributor.authorLam, KYen_HK
dc.contributor.authorTang, JCOen_HK
dc.contributor.authorSrivastava, Gen_HK
dc.date.accessioned2009-04-03T07:39:00Z-
dc.date.available2009-04-03T07:39:00Z-
dc.date.issued1999en_HK
dc.identifier.citationJournal of Clinical Pathology - Molecular Pathology, 1999, v. 52 n. 6, p. 353-356en_HK
dc.identifier.issn1366-8714en_HK
dc.identifier.urihttp://hdl.handle.net/10722/54184-
dc.description.abstractAIM: To investigate whether PTEN/MMAC1 mutations play a role in the carcinogenesis of oesophageal squamous cell carcinoma. METHODS: A panel of 33 primary oesophageal squamous cell carcinoma tumour samples and 20 corresponding morphologically normal tissues was examined for mutations in all nine exons of the PTEN/MMAC1 gene by means of polymerase chain reaction single strand conformational polymorphism analysis (PCR-SSCP) and direct DNA sequencing methods. RESULTS: Only one of 33 oesophageal squamous cell carcinomas showed an aberrant SSCP band. Further sequencing analysis of this sample revealed an 802 -29 T → C substitution in intron 7. PTEN/MMAC1 mutations were not found in the mutational 'hot spot' in exon 5, even after direct sequencing of six oesophageal squamous cell carcinoma samples and three normal tissues. However, a deletion of one nucleotide T at position 492 +8 in intron 5 was seen in all samples. CONCLUSION: These results suggest that PTEN/MMAC1 mutations do not play a major role in the carcinogenesis of oesophageal squamous cell carcinoma.en_HK
dc.languageengen_HK
dc.publisherBMJ Publishing Group. The Journal's web site is located at http://mp.bmjjournals.com/en_HK
dc.relation.ispartofJournal of Clinical Pathology - Molecular Pathologyen_HK
dc.rightsJournal of Clinical Pathology. Copyright © BMJ Publishing Group.en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectMutationen_HK
dc.subjectOesophageal carcinomaen_HK
dc.subjectPolymerase chain reaction single strand conformational polymorphism analysisen_HK
dc.subjectPTEN/MMAC1en_HK
dc.subject.meshCarcinoma, Squamous Cell - geneticsen_HK
dc.subject.meshEsophageal Neoplasms - geneticsen_HK
dc.subject.meshMutationen_HK
dc.subject.meshPhosphoric Monoester Hydrolases - geneticsen_HK
dc.subject.meshTumor Suppressor Proteinsen_HK
dc.titleMutational analysis of the PTEN/MMAC1 gene in primary oesophageal squamous cell carcinomasen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-9746&volume=52&issue=6&spage=353&epage=356&date=1999&atitle=Mutational+analysis+of+the+PTEN/MMAC1+gene+in+primary+oesophageal+squamous+cell+carcinomasen_HK
dc.identifier.emailLam, AKY: akylam@hkucc.hku.hken_HK
dc.identifier.emailSrivastava, G: gopesh@pathology.hku.hk-
dc.identifier.authoritySrivastava, G=rp00365en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.pmid10748870-
dc.identifier.pmcidPMC395722-
dc.identifier.scopuseid_2-s2.0-0033406380en_HK
dc.identifier.hkuros52633-
dc.identifier.hkuros56645-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033406380&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume52en_HK
dc.identifier.issue6en_HK
dc.identifier.spage353en_HK
dc.identifier.epage356en_HK
dc.publisher.placeUnited Kingdomen_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats