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Conference Paper: Melatonin protects neuronal cells against cell injury induced by oxygen-glucose deprivation

TitleMelatonin protects neuronal cells against cell injury induced by oxygen-glucose deprivation
Authors
KeywordsMELATONIN
IN VITRO
NEURONAL DEATH
ISCHEMIA
Issue Date2002
PublisherSociety for Neuroscience. The Journal's web site is located at http://sfn.scholarone.com/
Citation
Neuroscience 2002, Orlando, FL, 3-7 November 2002, Presentation no. 392.15 How to Cite?
AbstractMelatonin is a neurohormone secreted from the pineal gland. The present study was designed to study whether melatonin protects against cell injury due to oxygen-glucose deprivation (OGD) using cultured neuronal cells and whether the protection is mediated via melatonin membrane receptors. The neuronal SHSY5Y cells were seeded in 96-well microtiter plates for four days before subjected to 1 hour OGD. Melatonin at different doses was added to the medium beginning at 5 minutes before OGD. Twenty four hours after OGD, cell viability was quantitatively assessed by the measurement of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) in the medium at 24 h after OGD. The melatonin membrane receptors (mt1 and MT2) were detected using reverse transcription-polymerase chain reaction (RT-PCR). The results of RT-PCR show that neither mt1 nor MT2 membrane receptor was expressed in this neuronal cell line. Melatonin protects neuronal cells against OGD-induced cell injury in a dose-dependent manner. The protective action of melatonin is not mediated via its membrane receptors.
Persistent Identifierhttp://hdl.handle.net/10722/54143

 

DC FieldValueLanguage
dc.contributor.authorPei, Zen_HK
dc.contributor.authorCheung, RTFen_HK
dc.date.accessioned2009-04-03T07:37:52Z-
dc.date.available2009-04-03T07:37:52Z-
dc.date.issued2002en_HK
dc.identifier.citationNeuroscience 2002, Orlando, FL, 3-7 November 2002, Presentation no. 392.15en_HK
dc.identifier.urihttp://hdl.handle.net/10722/54143-
dc.description.abstractMelatonin is a neurohormone secreted from the pineal gland. The present study was designed to study whether melatonin protects against cell injury due to oxygen-glucose deprivation (OGD) using cultured neuronal cells and whether the protection is mediated via melatonin membrane receptors. The neuronal SHSY5Y cells were seeded in 96-well microtiter plates for four days before subjected to 1 hour OGD. Melatonin at different doses was added to the medium beginning at 5 minutes before OGD. Twenty four hours after OGD, cell viability was quantitatively assessed by the measurement of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) in the medium at 24 h after OGD. The melatonin membrane receptors (mt1 and MT2) were detected using reverse transcription-polymerase chain reaction (RT-PCR). The results of RT-PCR show that neither mt1 nor MT2 membrane receptor was expressed in this neuronal cell line. Melatonin protects neuronal cells against OGD-induced cell injury in a dose-dependent manner. The protective action of melatonin is not mediated via its membrane receptors.-
dc.languageengen_HK
dc.publisherSociety for Neuroscience. The Journal's web site is located at http://sfn.scholarone.com/en_HK
dc.relation.ispartofSociety for Neuroscience Annual Meeting-
dc.rightsSociety for Neuroscience Abstract viewer & itinerary planner. Copyright © Society for Neuroscience.en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectMELATONIN-
dc.subjectIN VITRO-
dc.subjectNEURONAL DEATH-
dc.subjectISCHEMIA-
dc.titleMelatonin protects neuronal cells against cell injury induced by oxygen-glucose deprivationen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailCheung, RTF: rtcheung@hku.hken_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.hkuros115180-

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