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Conference Paper: Melatonin pretreatment protects against focal cerebral ischemia in the rat

TitleMelatonin pretreatment protects against focal cerebral ischemia in the rat
Authors
KeywordsPINEAL
NEUROPROTECTION
STROKE
DOSE-RESPONSE
Issue Date2000
PublisherSociety for Neuroscience. The Journal's web site is located at http://sfn.scholarone.com/
Citation
Neuroscience 2000, New Orleans, LA, 4-9 November 2000, Presentation no. 284.6 How to Cite?
AbstractMelatonin (MT) possesses many properties of an ideal neuroprotectant. In this study, the neuroprotective effects of exogenous MT were tested in a middle cerebral artery occlusion (MCAO) stroke model. Adult male Sprague-Dawley rats (280 to 360 g) were anesthetized with sodium pentobarbital (60 mg/kg, I.P.) to undergo reversible right-sided endovascular MCAO for 3 hours. Arterial blood pressure, heart rate and cerebral blood flow (CBF) were monitored, and rectal temperature was kept between 36.5 and 37.5 ºC throughout anesthesia. One I.P. dose of MT (at 1.5, 5, or 15 mg/kg) or the vehicle was given 30 minutes before onset of ischemia. The rats were decapitated on day 3 of MCAO, and their brains were stained with 2% triphenyltetrazolium chloride for determination of infarction. Results were compared using 2-tailed student’s t test. When compared to the relative infarct volume of 31.8±3.3% (mean±SEM; 16 rats) in the control group, treatment with MT reduced the relative infarct volume in a dose-dependent manner (30.5±3.2% in the 1.5 mg/kg group [17 rats]; 15.9±2.2% in the 5 mg/kg group [16 rats], P < 0.05; 21.4±3.0% in the 15 mg/kg group [15 rats], P < 0.05). There was no significant difference in heart rate, arterial blood pressure and CBF among the groups. We concluded that a single dose of MT between 5 and 15 mg/kg protects against focal cerebral ischemia, when given 30 minutes before onset of ischemia. The above doses of MT do not produce significant hemodynamic effects nor alter the CBF during ischemia and reperfusion. Supported by the CRCG Research Grant 10202138 of the University of Hong Kong
Persistent Identifierhttp://hdl.handle.net/10722/54138

 

DC FieldValueLanguage
dc.contributor.authorHo, HTSen_HK
dc.contributor.authorPei, Zen_HK
dc.contributor.authorPang, SFen_HK
dc.contributor.authorCheung, RTFen_HK
dc.date.accessioned2009-04-03T07:37:45Z-
dc.date.available2009-04-03T07:37:45Z-
dc.date.issued2000en_HK
dc.identifier.citationNeuroscience 2000, New Orleans, LA, 4-9 November 2000, Presentation no. 284.6en_HK
dc.identifier.urihttp://hdl.handle.net/10722/54138-
dc.description.abstractMelatonin (MT) possesses many properties of an ideal neuroprotectant. In this study, the neuroprotective effects of exogenous MT were tested in a middle cerebral artery occlusion (MCAO) stroke model. Adult male Sprague-Dawley rats (280 to 360 g) were anesthetized with sodium pentobarbital (60 mg/kg, I.P.) to undergo reversible right-sided endovascular MCAO for 3 hours. Arterial blood pressure, heart rate and cerebral blood flow (CBF) were monitored, and rectal temperature was kept between 36.5 and 37.5 ºC throughout anesthesia. One I.P. dose of MT (at 1.5, 5, or 15 mg/kg) or the vehicle was given 30 minutes before onset of ischemia. The rats were decapitated on day 3 of MCAO, and their brains were stained with 2% triphenyltetrazolium chloride for determination of infarction. Results were compared using 2-tailed student’s t test. When compared to the relative infarct volume of 31.8±3.3% (mean±SEM; 16 rats) in the control group, treatment with MT reduced the relative infarct volume in a dose-dependent manner (30.5±3.2% in the 1.5 mg/kg group [17 rats]; 15.9±2.2% in the 5 mg/kg group [16 rats], P < 0.05; 21.4±3.0% in the 15 mg/kg group [15 rats], P < 0.05). There was no significant difference in heart rate, arterial blood pressure and CBF among the groups. We concluded that a single dose of MT between 5 and 15 mg/kg protects against focal cerebral ischemia, when given 30 minutes before onset of ischemia. The above doses of MT do not produce significant hemodynamic effects nor alter the CBF during ischemia and reperfusion. Supported by the CRCG Research Grant 10202138 of the University of Hong Kong-
dc.languageengen_HK
dc.publisherSociety for Neuroscience. The Journal's web site is located at http://sfn.scholarone.com/en_HK
dc.relation.ispartofSociety for Neuroscience Annual Meeting-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsSociety for Neuroscience Abstract viewer & itinerary planner. Copyright © Society for Neuroscience.en_HK
dc.subjectPINEAL-
dc.subjectNEUROPROTECTION-
dc.subjectSTROKE-
dc.subjectDOSE-RESPONSE-
dc.titleMelatonin pretreatment protects against focal cerebral ischemia in the raten_HK
dc.typeConference_Paperen_HK
dc.identifier.emailPang, SF: hrmypsf@hkucc.hku.hken_HK
dc.identifier.emailCheung, RTF: rtcheung@hku.hken_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.hkuros63483-

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