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Conference Paper: Effects of exogenous neuropeptide Y and neuropeptide Y-Y1 receptor antagonist on focal cerebral ischemia in the rat

TitleEffects of exogenous neuropeptide Y and neuropeptide Y-Y1 receptor antagonist on focal cerebral ischemia in the rat
Authors
KeywordsBIBP3226
NEUROPROTECTION
NPY
STROKE
Issue Date2000
PublisherSociety for Neuroscience. The Journal's web site is located at http://sfn.scholarone.com/
Citation
Neuroscience 2000, New Orleans, LA, 4-9 November 2000, Presentation no. 285.18 How to Cite?
AbstractWe studied the effects of exogenous neuropeptide Y (NPY) and BIBP3226, an NPY-Y1 antagonist, on the infarct volume. Adult male Sprague-Dawley rats weighing between 280 and 380 g were anaesthetised with sodium pentobarbital (60 mg/kg, I.P.) to undergo reversible right-sided endovascular middle cerebral artery occlusion (MCAO) for 2 hours. Arterial blood pressure, heart rate and cerebral blood flow (CBF) were monitored, and rectal temperature was kept between 36.5 and 37.5 ºC throughout anesthesia. One dose of NPY (10 or 70 μg/kg), BIBP3226 (5 or 15 μg/kg), or the vehicle was given via slow intra-cerebroventricular (ICV) injection 30 min after onset of ischemia. The rats were decapitated on day 3 of MCAO, and their brains were stained with 2% triphenyltetrazolium chloride for determination of infarction. Results were compared using 2-tailed student’s t test. When compared to the relative infarct volume of 15.7±3.6% (mean±SEM; 7 rats) in the control group, exogenous NPY treatment increased the relative infarct volumes (30.3±5.1% in the 10 μg/kg group [9 rats], P < 0.05; 24.1±3.8% in the 70 mg/kg group [8 rats]), whereas BIBP3226 treatment reduced the relative infarct volumes (9.7±2.5% in the 5 μg/kg group [7 rats]; 6.5±1.0% in the 15 μg/kg group [9 rats], P < 0.05). A major reduction in CBF during reperfusion was observed after exogenous NPY treatment, while there was no significant change in CBF after BIBP3226 administration. Our results suggest that pharmacological inhibition of NPY-Y1 receptor protects against ischemic injury without affecting local CBF during ischemia and reperfusion. Supported by the CRCG Research Grant 10202658 of the University of Hong Kong
Persistent Identifierhttp://hdl.handle.net/10722/54109

 

DC FieldValueLanguage
dc.contributor.authorChen, SHen_HK
dc.contributor.authorCheung, RTFen_HK
dc.date.accessioned2009-04-03T07:37:01Z-
dc.date.available2009-04-03T07:37:01Z-
dc.date.issued2000en_HK
dc.identifier.citationNeuroscience 2000, New Orleans, LA, 4-9 November 2000, Presentation no. 285.18en_HK
dc.identifier.urihttp://hdl.handle.net/10722/54109-
dc.description.abstractWe studied the effects of exogenous neuropeptide Y (NPY) and BIBP3226, an NPY-Y1 antagonist, on the infarct volume. Adult male Sprague-Dawley rats weighing between 280 and 380 g were anaesthetised with sodium pentobarbital (60 mg/kg, I.P.) to undergo reversible right-sided endovascular middle cerebral artery occlusion (MCAO) for 2 hours. Arterial blood pressure, heart rate and cerebral blood flow (CBF) were monitored, and rectal temperature was kept between 36.5 and 37.5 ºC throughout anesthesia. One dose of NPY (10 or 70 μg/kg), BIBP3226 (5 or 15 μg/kg), or the vehicle was given via slow intra-cerebroventricular (ICV) injection 30 min after onset of ischemia. The rats were decapitated on day 3 of MCAO, and their brains were stained with 2% triphenyltetrazolium chloride for determination of infarction. Results were compared using 2-tailed student’s t test. When compared to the relative infarct volume of 15.7±3.6% (mean±SEM; 7 rats) in the control group, exogenous NPY treatment increased the relative infarct volumes (30.3±5.1% in the 10 μg/kg group [9 rats], P < 0.05; 24.1±3.8% in the 70 mg/kg group [8 rats]), whereas BIBP3226 treatment reduced the relative infarct volumes (9.7±2.5% in the 5 μg/kg group [7 rats]; 6.5±1.0% in the 15 μg/kg group [9 rats], P < 0.05). A major reduction in CBF during reperfusion was observed after exogenous NPY treatment, while there was no significant change in CBF after BIBP3226 administration. Our results suggest that pharmacological inhibition of NPY-Y1 receptor protects against ischemic injury without affecting local CBF during ischemia and reperfusion. Supported by the CRCG Research Grant 10202658 of the University of Hong Kong-
dc.languageengen_HK
dc.publisherSociety for Neuroscience. The Journal's web site is located at http://sfn.scholarone.com/en_HK
dc.relation.ispartofSociety for Neuroscience Annual Meeting-
dc.rightsSociety for Neuroscience Abstract viewer & itinerary planner. Copyright © Society for Neuroscience.en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectBIBP3226-
dc.subjectNEUROPROTECTION-
dc.subjectNPY-
dc.subjectSTROKE-
dc.titleEffects of exogenous neuropeptide Y and neuropeptide Y-Y1 receptor antagonist on focal cerebral ischemia in the raten_HK
dc.typeConference_Paperen_HK
dc.identifier.emailCheung, RTF: rtcheung@hku.hken_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.hkuros63481-

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