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Article: Optimization of laccase production by Pycnoporus sanguineus in submerged liquid culture

TitleOptimization of laccase production by Pycnoporus sanguineus in submerged liquid culture
Authors
KeywordsBasidiomycete
Laccase
Pycnoporus sanguineus
White-rot fungus
Issue Date2000
PublisherMycological Society of America. The Journal's web site is located at http://www.msafungi.org
Citation
Mycologia, 2000, v. 92 n. 1, p. 139-144 How to Cite?
AbstractThe white-rot fungus Pycnoporus sanguineus produces laccase under a range of C/N ratios in submerged liquid culture. Enzyme production was increased 50 fold in the presence of 20 μM xylidine to a maximum of 1368 U L-1 in a high carbon low nitrogen medium. Slight repression of enzyme production was observed in high nitrogen culture medium. Other potential inducers were less effective (Tween 80, wood fibres) and reduced the stimulation observed by xylidine alone when included in the same culture medium. Veratryl alcohol failed to stimulate laccase production. Activity of the enzyme activity in crude culture nitrate was stable at temperatures of 35 C and below with a pH optimum of 3.0. The laccase of P. sanguineus was identified as a ca 65 kDa protein produced as multiple isoforms.
Persistent Identifierhttp://hdl.handle.net/10722/53369
ISSN
2015 Impact Factor: 2.638
2015 SCImago Journal Rankings: 1.145
References

 

DC FieldValueLanguage
dc.contributor.authorPointing, SBen_HK
dc.contributor.authorJones, EBGen_HK
dc.contributor.authorVrijmoed, LLPen_HK
dc.date.accessioned2009-04-03T07:17:55Z-
dc.date.available2009-04-03T07:17:55Z-
dc.date.issued2000en_HK
dc.identifier.citationMycologia, 2000, v. 92 n. 1, p. 139-144en_HK
dc.identifier.issn0027-5514en_HK
dc.identifier.urihttp://hdl.handle.net/10722/53369-
dc.description.abstractThe white-rot fungus Pycnoporus sanguineus produces laccase under a range of C/N ratios in submerged liquid culture. Enzyme production was increased 50 fold in the presence of 20 μM xylidine to a maximum of 1368 U L-1 in a high carbon low nitrogen medium. Slight repression of enzyme production was observed in high nitrogen culture medium. Other potential inducers were less effective (Tween 80, wood fibres) and reduced the stimulation observed by xylidine alone when included in the same culture medium. Veratryl alcohol failed to stimulate laccase production. Activity of the enzyme activity in crude culture nitrate was stable at temperatures of 35 C and below with a pH optimum of 3.0. The laccase of P. sanguineus was identified as a ca 65 kDa protein produced as multiple isoforms.en_HK
dc.languageengen_HK
dc.publisherMycological Society of America. The Journal's web site is located at http://www.msafungi.orgen_HK
dc.relation.ispartofMycologiaen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectBasidiomyceteen_HK
dc.subjectLaccaseen_HK
dc.subjectPycnoporus sanguineusen_HK
dc.subjectWhite-rot fungusen_HK
dc.titleOptimization of laccase production by Pycnoporus sanguineus in submerged liquid cultureen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0027-5514&volume=92&issue=1&spage=139&epage=144&date=2000&atitle=Optimization+of+laccase+production+by+Pycnoporus+sanguineus+in+submerged+liquid+cultureen_HK
dc.identifier.emailPointing, SB: pointing@hkucc.hku.hken_HK
dc.identifier.authorityPointing, SB=rp00771en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.scopuseid_2-s2.0-0000515906en_HK
dc.identifier.hkuros48782-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0000515906&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume92en_HK
dc.identifier.issue1en_HK
dc.identifier.spage139en_HK
dc.identifier.epage144en_HK
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridPointing, SB=6603986412en_HK
dc.identifier.scopusauthoridJones, EBG=24760500100en_HK
dc.identifier.scopusauthoridVrijmoed, LLP=7003337180en_HK

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