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Article: Different cis-regulatory DNA elements mediate developmental stage- and tissue-specific expression of the human COL2A1 gene in transgenic mice

TitleDifferent cis-regulatory DNA elements mediate developmental stage- and tissue-specific expression of the human COL2A1 gene in transgenic mice
Authors
Issue Date1998
PublisherRockefeller University Press. The Journal's web site is located at http://www.jcb.org
Citation
Journal Of Cell Biology, 1998, v. 141 n. 6, p. 1291-1300 How to Cite?
AbstractExpression of the type II collagen gene (human COL2A1, mouse Col2a1) heralds the differentiation of chondrocytes. It is also expressed in progenitor cells of some nonchondrogenic tissues during embryogenesis. DNA sequences in the 5' flanking region and intron 1 are known to control tissue- specific expression in vitro, but the regulation of COL2A1 expression in vivo is not clearly understood. We have tested the regulatory activity of DNA sequences from COL2A1 on the expression of a lacZ reporter gene in transgenic mice. We have found that type II collagen characteristic expression of the transgene requires the enhancer activity of a 309-bp fragment (+2,388 to +2,696) in intron 1 in conjunction with 6.1-kb 5' sequences. Different regulatory elements were found in the 1.6-kb region (+701 to +2,387) of intron 1 which only needs 90-bp 5' sequences for tissue-specific expression in different components of the developing cartilaginous skeleton. Distinct positive and negative regulatory elements act together to control tissue- specific transgene expression in the developing midbrain neuroepithelium. Positive elements affecting expression in the midbrain were found in the region from -90 to -1,500 and from +701 to +2,387, whereas negatively acting elements were detected in the regions from -1,500 to -6,100 and +2,388 to +2,855.
Persistent Identifierhttp://hdl.handle.net/10722/53320
ISSN
2015 Impact Factor: 8.717
2015 SCImago Journal Rankings: 7.923
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLeung, KKHen_HK
dc.contributor.authorNg, LJen_HK
dc.contributor.authorHo, KKYen_HK
dc.contributor.authorTam, PPLen_HK
dc.contributor.authorCheah, KSEen_HK
dc.date.accessioned2009-04-03T07:12:17Z-
dc.date.available2009-04-03T07:12:17Z-
dc.date.issued1998en_HK
dc.identifier.citationJournal Of Cell Biology, 1998, v. 141 n. 6, p. 1291-1300en_HK
dc.identifier.issn0021-9525en_HK
dc.identifier.urihttp://hdl.handle.net/10722/53320-
dc.description.abstractExpression of the type II collagen gene (human COL2A1, mouse Col2a1) heralds the differentiation of chondrocytes. It is also expressed in progenitor cells of some nonchondrogenic tissues during embryogenesis. DNA sequences in the 5' flanking region and intron 1 are known to control tissue- specific expression in vitro, but the regulation of COL2A1 expression in vivo is not clearly understood. We have tested the regulatory activity of DNA sequences from COL2A1 on the expression of a lacZ reporter gene in transgenic mice. We have found that type II collagen characteristic expression of the transgene requires the enhancer activity of a 309-bp fragment (+2,388 to +2,696) in intron 1 in conjunction with 6.1-kb 5' sequences. Different regulatory elements were found in the 1.6-kb region (+701 to +2,387) of intron 1 which only needs 90-bp 5' sequences for tissue-specific expression in different components of the developing cartilaginous skeleton. Distinct positive and negative regulatory elements act together to control tissue- specific transgene expression in the developing midbrain neuroepithelium. Positive elements affecting expression in the midbrain were found in the region from -90 to -1,500 and from +701 to +2,387, whereas negatively acting elements were detected in the regions from -1,500 to -6,100 and +2,388 to +2,855.en_HK
dc.languageengen_HK
dc.publisherRockefeller University Press. The Journal's web site is located at http://www.jcb.orgen_HK
dc.relation.ispartofJournal of Cell Biologyen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsThe Journal of Cell Biology. Copyright © Rockefeller University Press.en_HK
dc.subject.meshCollagen - geneticsen_HK
dc.subject.meshGene Expression Regulationen_HK
dc.subject.meshRegulatory Sequences, Nucleic Aciden_HK
dc.subject.meshEmbryonic and Fetal Developmenten_HK
dc.subject.meshMesencephalon - metabolismen_HK
dc.titleDifferent cis-regulatory DNA elements mediate developmental stage- and tissue-specific expression of the human COL2A1 gene in transgenic miceen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-9525&volume=141&issue=6&spage=1291&epage=1300&date=1998&atitle=Different+cis-regulatory+DNA+elements+mediate+developmental+stage-+and+tissue-specific+expression+of+the+human+COL2A1+gene+in+transgenic+miceen_HK
dc.identifier.emailLeung, KKH:keithlee@hku.hken_HK
dc.identifier.emailCheah, KSE:hrmbdkc@hku.hken_HK
dc.identifier.authorityLeung, KKH=rp00298en_HK
dc.identifier.authorityCheah, KSE=rp00342en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1083/jcb.141.6.1291en_HK
dc.identifier.pmid9628886-
dc.identifier.pmcidPMC2132792-
dc.identifier.scopuseid_2-s2.0-2642634800en_HK
dc.identifier.hkuros36647-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-2642634800&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume141en_HK
dc.identifier.issue6en_HK
dc.identifier.spage1291en_HK
dc.identifier.epage1300en_HK
dc.identifier.isiWOS:000074303600001-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLeung, KKH=7401860467en_HK
dc.identifier.scopusauthoridNg, LJ=7201477760en_HK
dc.identifier.scopusauthoridHo, KKY=36819420500en_HK
dc.identifier.scopusauthoridTam, PPL=7202539412en_HK
dc.identifier.scopusauthoridCheah, KSE=35387746200en_HK

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