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Article: Myosin-1a is critical for normal brush border structure and composition

TitleMyosin-1a is critical for normal brush border structure and composition
Authors
Issue Date2005
PublisherAmerican Society for Cell Biology. The Journal's web site is located at http://www.molbiolcell.org/
Citation
Molecular Biology Of The Cell, 2005, v. 16 n. 5, p. 2443-2457 How to Cite?
AbstractTo develop our understanding of myosin-1a function in vivo, we have created a mouse line null for the myosin-1a gene. Myosin-1a knockout mice demonstrate no overt phenotypes at the whole animal level but exhibit significant perturbations and signs of stress at the cellular level. Among these are defects in microvillar membrane morphology, distinct changes in brush-border organization, loss of numerous cytoskeletal and membrane components from the brush border, and redistribution of intermediate filament proteins into the brush border. We also observed significant ectopic recruitment of another short-tailed class I motor, myosin-1c, into the brush border of knockout enterocytes. This latter finding, a clear demonstration of functional redundancy among vertebrate myosins-I, may account for the lack of a whole animal phenotype. Nevertheless, these results indicate that myosin-1a is a critical multifunctional component of the enterocyte, required for maintaining the normal composition and highly ordered structure of the brush border. © 2005 by The American Society for Cell Biology.
Persistent Identifierhttp://hdl.handle.net/10722/53316
ISSN
2015 Impact Factor: 4.037
2015 SCImago Journal Rankings: 3.665
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorTyska, MJen_HK
dc.contributor.authorMackey, ATen_HK
dc.contributor.authorHuang, JDen_HK
dc.contributor.authorCopeland, NGen_HK
dc.contributor.authorJenkins, NAen_HK
dc.contributor.authorMooseker, MSen_HK
dc.date.accessioned2009-04-03T07:12:10Z-
dc.date.available2009-04-03T07:12:10Z-
dc.date.issued2005en_HK
dc.identifier.citationMolecular Biology Of The Cell, 2005, v. 16 n. 5, p. 2443-2457en_HK
dc.identifier.issn1059-1524en_HK
dc.identifier.urihttp://hdl.handle.net/10722/53316-
dc.description.abstractTo develop our understanding of myosin-1a function in vivo, we have created a mouse line null for the myosin-1a gene. Myosin-1a knockout mice demonstrate no overt phenotypes at the whole animal level but exhibit significant perturbations and signs of stress at the cellular level. Among these are defects in microvillar membrane morphology, distinct changes in brush-border organization, loss of numerous cytoskeletal and membrane components from the brush border, and redistribution of intermediate filament proteins into the brush border. We also observed significant ectopic recruitment of another short-tailed class I motor, myosin-1c, into the brush border of knockout enterocytes. This latter finding, a clear demonstration of functional redundancy among vertebrate myosins-I, may account for the lack of a whole animal phenotype. Nevertheless, these results indicate that myosin-1a is a critical multifunctional component of the enterocyte, required for maintaining the normal composition and highly ordered structure of the brush border. © 2005 by The American Society for Cell Biology.en_HK
dc.languageengen_HK
dc.publisherAmerican Society for Cell Biology. The Journal's web site is located at http://www.molbiolcell.org/en_HK
dc.relation.ispartofMolecular Biology of the Cellen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsMolecular Biology of the Cell. Copyright © American Society for Cell Biology.en_HK
dc.subject.meshEnterocytes - metabolism - ultrastructureen_HK
dc.subject.meshMicrovilli - metabolism - ultrastructureen_HK
dc.subject.meshMyosin Heavy Chains - deficiency - genetics - metabolismen_HK
dc.subject.meshColon - metabolism - ultrastructureen_HK
dc.subject.meshMice, Knockouten_HK
dc.titleMyosin-1a is critical for normal brush border structure and compositionen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1059-1524&volume=16&issue=5&spage=2443&epage=2457&date=2005&atitle=Myosin-1a+is+critical+for+normal+brush+border+structure+and+compositionen_HK
dc.identifier.emailHuang, JD:jdhuang@hkucc.hku.hken_HK
dc.identifier.authorityHuang, JD=rp00451en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1091/mbc.E04-12-1116en_HK
dc.identifier.pmid15758024-
dc.identifier.pmcidPMC1087248-
dc.identifier.scopuseid_2-s2.0-18244409866en_HK
dc.identifier.hkuros98290-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-18244409866&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume16en_HK
dc.identifier.issue5en_HK
dc.identifier.spage2443en_HK
dc.identifier.epage2457en_HK
dc.identifier.isiWOS:000228737400026-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridTyska, MJ=6603271989en_HK
dc.identifier.scopusauthoridMackey, AT=7006250183en_HK
dc.identifier.scopusauthoridHuang, JD=8108660600en_HK
dc.identifier.scopusauthoridCopeland, NG=17034265300en_HK
dc.identifier.scopusauthoridJenkins, NA=35379887700en_HK
dc.identifier.scopusauthoridMooseker, MS=7006520381en_HK
dc.identifier.citeulike3130172-

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