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Article: Expression of the Hevea brasiliensis (H.B.K.) Mull. Arg. 3-hydroxy-3-methylglutaryl-coenzyme A reductase 1 in tobacco results in sterol overproduction

TitleExpression of the Hevea brasiliensis (H.B.K.) Mull. Arg. 3-hydroxy-3-methylglutaryl-coenzyme A reductase 1 in tobacco results in sterol overproduction
Authors
KeywordsBiology
Botany
Issue Date1995
PublisherAmerican Society of Plant Biologists. The Journal's web site is located at http://www.plantphysiol.org
Citation
Plant Physiology, 1995, v. 109 n. 3, p. 761-770 How to Cite?
AbstractA genomic fragment encoding one (HMGR1) of the three 3-hydroxy-3-methylglutaryl coenzyme A reductases (HMGRs) from Hevea brasiliensis (H.B.K.) Mull. Arg. (M.-L. Chye, C.-T. Tan, N.-H. Chua [1992] Plant Mol Biol 19: 473-484) was introduced into Nicotiana tabacum L. cv xanthi via Agrobacterium transformation to study the influence of the hmg1 gene product on plant isoprenoid biosynthesis. Transgenic plants were morphologically indistinguishable from control wild-type plants and displayed the same developmental pattern. Transgenic lines showed an increase in the level of total sterols up to 6-fold, probably because of an increased expression level of hmg1 mRNA and a corresponding increased enzymatic activity for HMGR, when compared with the level of total sterols from control lines not expressing the hmg1 transgene. In addition to the pathway end products, campesterol, sitosterol, and stigmasterol, some biosynthetic intermediates such as cycloartenol also accumulated in transgenic tissues. Most of the overproduced sterols were detected as steryl-esters and were likely to be stored in cytoplasmic lipid bodies. These data strongly support the conclusion that plant HMGR is a key limiting enzyme in phytosterol biosynthesis.
Persistent Identifierhttp://hdl.handle.net/10722/49435
ISSN
2015 Impact Factor: 6.28
2015 SCImago Journal Rankings: 3.642
PubMed Central ID

 

DC FieldValueLanguage
dc.contributor.authorSchaller, Hen_HK
dc.contributor.authorGrausem, Ben_HK
dc.contributor.authorBenveniste, Pen_HK
dc.contributor.authorChye MeeLenen_HK
dc.contributor.authorTan ChioTeeen_HK
dc.contributor.authorSong YuHuaen_HK
dc.contributor.authorChua NamHaien_HK
dc.date.accessioned2008-06-12T06:42:34Z-
dc.date.available2008-06-12T06:42:34Z-
dc.date.issued1995en_HK
dc.identifier.citationPlant Physiology, 1995, v. 109 n. 3, p. 761-770en_HK
dc.identifier.issn0032-0889en_HK
dc.identifier.urihttp://hdl.handle.net/10722/49435-
dc.description.abstractA genomic fragment encoding one (HMGR1) of the three 3-hydroxy-3-methylglutaryl coenzyme A reductases (HMGRs) from Hevea brasiliensis (H.B.K.) Mull. Arg. (M.-L. Chye, C.-T. Tan, N.-H. Chua [1992] Plant Mol Biol 19: 473-484) was introduced into Nicotiana tabacum L. cv xanthi via Agrobacterium transformation to study the influence of the hmg1 gene product on plant isoprenoid biosynthesis. Transgenic plants were morphologically indistinguishable from control wild-type plants and displayed the same developmental pattern. Transgenic lines showed an increase in the level of total sterols up to 6-fold, probably because of an increased expression level of hmg1 mRNA and a corresponding increased enzymatic activity for HMGR, when compared with the level of total sterols from control lines not expressing the hmg1 transgene. In addition to the pathway end products, campesterol, sitosterol, and stigmasterol, some biosynthetic intermediates such as cycloartenol also accumulated in transgenic tissues. Most of the overproduced sterols were detected as steryl-esters and were likely to be stored in cytoplasmic lipid bodies. These data strongly support the conclusion that plant HMGR is a key limiting enzyme in phytosterol biosynthesis.en_HK
dc.format.extent420 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherAmerican Society of Plant Biologists. The Journal's web site is located at http://www.plantphysiol.orgen_HK
dc.relation.ispartofPlant Physiologyen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectBiologyen_HK
dc.subjectBotanyen_HK
dc.titleExpression of the Hevea brasiliensis (H.B.K.) Mull. Arg. 3-hydroxy-3-methylglutaryl-coenzyme A reductase 1 in tobacco results in sterol overproductionen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0032-0889&volume=109&issue=3&spage=761&epage=770&date=1995&atitle=Expression+of+the+Hevea+brasiliensis+(H.B.K.)+Mull.+Arg.+3-hydroxy-3-methylglutaryl-coenzyme+A+reductase+1+in+tobacco+results+in+sterol+overproductionen_HK
dc.identifier.emailChye MeeLen: mlchye@hkucc.hku.hken_HK
dc.identifier.authorityChye MeeLen=rp00687en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.pmid12228630en_HK
dc.identifier.pmcidPMC161375-
dc.identifier.scopuseid_2-s2.0-0029199868en_HK
dc.identifier.hkuros7591-
dc.identifier.volume109en_HK
dc.identifier.issue3en_HK
dc.identifier.spage761en_HK
dc.identifier.epage770en_HK
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridSchaller, H=20035801800en_HK
dc.identifier.scopusauthoridGrausem, B=6507405609en_HK
dc.identifier.scopusauthoridBenveniste, P=7006330101en_HK
dc.identifier.scopusauthoridChye MeeLen=7003905460en_HK
dc.identifier.scopusauthoridTan ChioTee=7409770621en_HK
dc.identifier.scopusauthoridSong YuHua=7409933765en_HK
dc.identifier.scopusauthoridChua NamHai=7409669496en_HK

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