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Article: Gene essentiality determines chromosome organisation in bacteria

TitleGene essentiality determines chromosome organisation in bacteria
Authors
Issue Date2003
PublisherOxford University Press. The Journal's web site is located at http://nar.oxfordjournals.org/
Citation
Nucleic Acids Research, 2003, v. 31 n. 22, p. 6570-6577 How to Cite?
AbstractIn Escherichia coli and Bacillus subtilis, essentiality, not expressivity, drives the distribution of genes between the two replicating strands. Although essential genes tend to be coded in the leading replicating strand, the underlying selective constraints and the evolutionary extent of these findings have still not been subject to comparative studies. Here, we extend our previous analysis to the genomes of low G + C firmicutes and gamma-proteobacteria, and in a second step to all sequenced bacterial genomes. The inference of essentiality by homology allows us to show that essential genes are much more frequent in the leading strand than other genes, even when compared with non- essential highly expressed genes. Smaller biases were found in the genomes of obligatory intracellular bacteria, for which the assignment of essentiality by homology from fast growing free-living bacteria is most problematic. Cross-comparisons used to assess potential errors in the assignment of essentiality by homology revealed that, in most cases, variations in the assignment criteria have little influence on the overall results. Essential genes tend to be more conserved in the leading strand than average genes, which is consistent with selection for this positioning and may impose a strong constraint on chromosomal rearrangements. These results indicate that essentiality plays a fundamental role in the distribution of genes in most bacterial genomes.
Persistent Identifierhttp://hdl.handle.net/10722/49425
ISSN
2015 Impact Factor: 9.202
2015 SCImago Journal Rankings: 7.458
PubMed Central ID
ISI Accession Number ID
Errata

 

DC FieldValueLanguage
dc.contributor.authorRocha, EPCen_HK
dc.contributor.authorDanchin, Aen_HK
dc.date.accessioned2008-06-12T06:42:16Z-
dc.date.available2008-06-12T06:42:16Z-
dc.date.issued2003en_HK
dc.identifier.citationNucleic Acids Research, 2003, v. 31 n. 22, p. 6570-6577en_HK
dc.identifier.issn0305-1048en_HK
dc.identifier.urihttp://hdl.handle.net/10722/49425-
dc.description.abstractIn Escherichia coli and Bacillus subtilis, essentiality, not expressivity, drives the distribution of genes between the two replicating strands. Although essential genes tend to be coded in the leading replicating strand, the underlying selective constraints and the evolutionary extent of these findings have still not been subject to comparative studies. Here, we extend our previous analysis to the genomes of low G + C firmicutes and gamma-proteobacteria, and in a second step to all sequenced bacterial genomes. The inference of essentiality by homology allows us to show that essential genes are much more frequent in the leading strand than other genes, even when compared with non- essential highly expressed genes. Smaller biases were found in the genomes of obligatory intracellular bacteria, for which the assignment of essentiality by homology from fast growing free-living bacteria is most problematic. Cross-comparisons used to assess potential errors in the assignment of essentiality by homology revealed that, in most cases, variations in the assignment criteria have little influence on the overall results. Essential genes tend to be more conserved in the leading strand than average genes, which is consistent with selection for this positioning and may impose a strong constraint on chromosomal rearrangements. These results indicate that essentiality plays a fundamental role in the distribution of genes in most bacterial genomes.en_HK
dc.format.extent386 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherOxford University Press. The Journal's web site is located at http://nar.oxfordjournals.org/en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshBacteria - geneticsen_HK
dc.subject.meshChromosomes, Bacterial - geneticsen_HK
dc.subject.meshGenes, Bacterial - geneticsen_HK
dc.subject.meshGenes, Essential - geneticsen_HK
dc.subject.meshGenome, Bacterialen_HK
dc.titleGene essentiality determines chromosome organisation in bacteriaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0305-1048&volume=31&issue=22&spage=6570&epage=6577&date=2003&atitle=Gene+essentiality+determines+chromosome+organisation+in+bacteriaen_HK
dc.identifier.emailDanchin, A: adanchin@hkucc.hku.hken_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1093/nar/gkg859en_HK
dc.identifier.pmid14602916-
dc.identifier.pmcidPMC275555en_HK
dc.identifier.scopuseid_2-s2.0-0344668838-
dc.identifier.hkuros95419-
dc.identifier.isiWOS:000186590600021-
dc.relation.erratumdoi:10.1093/nar/gkg939-
dc.identifier.citeulike245962-

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