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Article: Methylation status of the Epstein-Barr virus major latent promoter C in latrogenic B cell lymphoproliferative disease: Application of PCR-based analysis

TitleMethylation status of the Epstein-Barr virus major latent promoter C in latrogenic B cell lymphoproliferative disease: Application of PCR-based analysis
Authors
Issue Date1999
PublisherAmerican Society for Investigative Pathology. The Journal's web site is located at http://www.amjpathol.org
Citation
American Journal Of Pathology, 1999, v. 155 n. 2, p. 619-625 How to Cite?
AbstractThe Epstein-Barr virus (EBV) major latent promoter C drives the expression of viral nuclear proteins important in lymphocyte immortalization and as targets for immune surveillance by cytotoxic T cells. Hypermethylation of the C promoter silences its transcription. This promoter is methylated and silent in Burkitt's lymphoma, Hodgkin's disease, nasopharyngeal carcinoma, and nasal lymphoma. However, it is never methylated in the EBV-immortalized lymphoblastoid cell lines that serve as a model for EBV-associated lymphoproliferative disease. We have analyzed C promoter methylation in iatrogenic EBV-associated B-cell lymphoproliferative disease, mainly posttransplant lymphoma, using a sensitive polymerase chain reaction-based C promoter methylation assay. Our results showed heterogeneity in lymphoproliferative disease with methylation of vital DNA in specimens from 3 of 13 patients. In specimens from two of these patients, only methylated viral DNA was detected and vital nuclear antigen expression was correspondingly restricted. Heterogeneity in C promoter methylation and expression of associated transcripts may be an important determinant of the growth properties of lymphoproliferative lesions and may provide an explanation for the failure of some tumors to respond to withdrawal or reduction of immunosuppressive therapy.
Persistent Identifierhttp://hdl.handle.net/10722/49417
ISSN
2015 Impact Factor: 4.206
2015 SCImago Journal Rankings: 2.653
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorQian, Ten_HK
dc.contributor.authorSwinnen, LJen_HK
dc.contributor.authorYang, Jen_HK
dc.contributor.authorSrivastava, Gen_HK
dc.contributor.authorRobertson, KDen_HK
dc.contributor.authorAmbinder, RFen_HK
dc.date.accessioned2008-06-12T06:42:03Z-
dc.date.available2008-06-12T06:42:03Z-
dc.date.issued1999en_HK
dc.identifier.citationAmerican Journal Of Pathology, 1999, v. 155 n. 2, p. 619-625en_HK
dc.identifier.issn0002-9440en_HK
dc.identifier.urihttp://hdl.handle.net/10722/49417-
dc.description.abstractThe Epstein-Barr virus (EBV) major latent promoter C drives the expression of viral nuclear proteins important in lymphocyte immortalization and as targets for immune surveillance by cytotoxic T cells. Hypermethylation of the C promoter silences its transcription. This promoter is methylated and silent in Burkitt's lymphoma, Hodgkin's disease, nasopharyngeal carcinoma, and nasal lymphoma. However, it is never methylated in the EBV-immortalized lymphoblastoid cell lines that serve as a model for EBV-associated lymphoproliferative disease. We have analyzed C promoter methylation in iatrogenic EBV-associated B-cell lymphoproliferative disease, mainly posttransplant lymphoma, using a sensitive polymerase chain reaction-based C promoter methylation assay. Our results showed heterogeneity in lymphoproliferative disease with methylation of vital DNA in specimens from 3 of 13 patients. In specimens from two of these patients, only methylated viral DNA was detected and vital nuclear antigen expression was correspondingly restricted. Heterogeneity in C promoter methylation and expression of associated transcripts may be an important determinant of the growth properties of lymphoproliferative lesions and may provide an explanation for the failure of some tumors to respond to withdrawal or reduction of immunosuppressive therapy.en_HK
dc.format.extent388 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherAmerican Society for Investigative Pathology. The Journal's web site is located at http://www.amjpathol.orgen_HK
dc.relation.ispartofAmerican Journal of Pathologyen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshDNA-Binding Proteins - geneticsen_HK
dc.subject.meshLeukemia, Promyelocytic, Acute - genetics - pathology - physiopathologyen_HK
dc.subject.meshOncogene Proteins, Fusion - geneticsen_HK
dc.subject.meshReceptors, Retinoic Acid - geneticsen_HK
dc.subject.meshTranscription Factors - geneticsen_HK
dc.titleMethylation status of the Epstein-Barr virus major latent promoter C in latrogenic B cell lymphoproliferative disease: Application of PCR-based analysisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0002-9440&volume=155&issue=2&spage=619&epage=625&date=1999&atitle=Methylation+status+of+the+Epstein-Barr+virus+major+latent+promoter+C+in+latrogenic+B+cell+lymphoproliferative+disease:+application+of+PCR-based+analysisen_HK
dc.identifier.emailSrivastava, G:gopesh@pathology.hku.hken_HK
dc.identifier.authoritySrivastava, G=rp00365en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.pmid10433954en_HK
dc.identifier.pmcidPMC1866850en_HK
dc.identifier.scopuseid_2-s2.0-0344980092en_HK
dc.identifier.hkuros52625-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0344980092&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume155en_HK
dc.identifier.issue2en_HK
dc.identifier.spage619en_HK
dc.identifier.epage625en_HK
dc.identifier.isiWOS:000081901600031-
dc.publisher.placeUnited Statesen_HK

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