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Article: Detection of Epstein-Barr virus-infected mucosal lymphocytes in nasal polyps

TitleDetection of Epstein-Barr virus-infected mucosal lymphocytes in nasal polyps
Authors
Issue Date1996
PublisherAmerican Society for Investigative Pathology. The Journal's web site is located at http://www.amjpathol.org
Citation
American Journal Of Pathology, 1996, v. 149 n. 4, p. 1111-1118 How to Cite?
AbstractPrimary nasal lymphomas of T or NK cell origin are known to be associated with Epstein-Barr virus (EBV). However, it is not known whether EBV is normally present in nasal mucosa as distinct to nasopharyngeal tissue. This study investigates the prevalence of EBV infection in 13 cases of nasal polyps. EBV DNA was detected in 2 of 13 (15%) by Southern blot hybridization and in 9 of 13 (69%) by polymerase chain reaction. In situ hybridization for EBV-encoded small nuclear RNAs (EBER) was positive in 11 of 13 (85%) cases; the virus was present in stromal lymphocytes only and not in the epithelial cells. Immunohistochemistry for EBV proteins in 7 cases revealed EBV nuclear antigen (EBNA)-2, latent membrane protein (LMP)-1, and ZEBRA (the switch protein encoded by gene BZLF1) expression in rare isolated stromal lymphocytes in 3 cases. Double immunostaining in 1 case showed that the LMP- 1+ cells were B or T cells. Immunohistochemistry for EBV lytic proteins showed very rare vital capsid antigen (VCA)+ and membrane antigen (MA)+ cells in 1 case and very rare diffuse early antigen (EA-D)+ and VCA+ cells in 1 other case. The expression of ZEBRA, EA-D, VCA, and MA suggested a disruption of latency in very rare stromal lymphocytes leading to a productive cycle. Although the incidence of EBV positivity in nasal polyps in our population is high (85%), very low numbers of EBV+ cells are found in each case. Nevertheless, they indicate that nasal mucosa could be one of the sites of EBV persistence through a low level of infection of the resident lymphocytes and thereby provide a possible setting for the emergence of virally associated tumors in this site.
Persistent Identifierhttp://hdl.handle.net/10722/49407
ISSN
2015 Impact Factor: 4.206
2015 SCImago Journal Rankings: 2.653
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorTao, Qen_HK
dc.contributor.authorSrivastava, Gen_HK
dc.contributor.authorDickens, Pen_HK
dc.contributor.authorHo, FCSen_HK
dc.date.accessioned2008-06-12T06:41:49Z-
dc.date.available2008-06-12T06:41:49Z-
dc.date.issued1996en_HK
dc.identifier.citationAmerican Journal Of Pathology, 1996, v. 149 n. 4, p. 1111-1118en_HK
dc.identifier.issn0002-9440en_HK
dc.identifier.urihttp://hdl.handle.net/10722/49407-
dc.description.abstractPrimary nasal lymphomas of T or NK cell origin are known to be associated with Epstein-Barr virus (EBV). However, it is not known whether EBV is normally present in nasal mucosa as distinct to nasopharyngeal tissue. This study investigates the prevalence of EBV infection in 13 cases of nasal polyps. EBV DNA was detected in 2 of 13 (15%) by Southern blot hybridization and in 9 of 13 (69%) by polymerase chain reaction. In situ hybridization for EBV-encoded small nuclear RNAs (EBER) was positive in 11 of 13 (85%) cases; the virus was present in stromal lymphocytes only and not in the epithelial cells. Immunohistochemistry for EBV proteins in 7 cases revealed EBV nuclear antigen (EBNA)-2, latent membrane protein (LMP)-1, and ZEBRA (the switch protein encoded by gene BZLF1) expression in rare isolated stromal lymphocytes in 3 cases. Double immunostaining in 1 case showed that the LMP- 1+ cells were B or T cells. Immunohistochemistry for EBV lytic proteins showed very rare vital capsid antigen (VCA)+ and membrane antigen (MA)+ cells in 1 case and very rare diffuse early antigen (EA-D)+ and VCA+ cells in 1 other case. The expression of ZEBRA, EA-D, VCA, and MA suggested a disruption of latency in very rare stromal lymphocytes leading to a productive cycle. Although the incidence of EBV positivity in nasal polyps in our population is high (85%), very low numbers of EBV+ cells are found in each case. Nevertheless, they indicate that nasal mucosa could be one of the sites of EBV persistence through a low level of infection of the resident lymphocytes and thereby provide a possible setting for the emergence of virally associated tumors in this site.en_HK
dc.format.extent418 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherAmerican Society for Investigative Pathology. The Journal's web site is located at http://www.amjpathol.orgen_HK
dc.relation.ispartofAmerican Journal of Pathologyen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshHerpesviridae Infections - virologyen_HK
dc.subject.meshHerpesvirus 4, Human - isolation & purificationen_HK
dc.subject.meshLymphocytes - virologyen_HK
dc.subject.meshNasal Mucosa - virologyen_HK
dc.subject.meshNasal Polyps - virologyen_HK
dc.titleDetection of Epstein-Barr virus-infected mucosal lymphocytes in nasal polypsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0002-9440&volume=149&issue=4&spage=1111&epage=1118&date=1996&atitle=Detection+of+Epstein-Barr+virus-infected+mucosal+lymphocytes+in+nasal+polypsen_HK
dc.identifier.emailSrivastava, G:gopesh@pathology.hku.hken_HK
dc.identifier.authoritySrivastava, G=rp00365en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.pmid8863660-
dc.identifier.pmcidPMC1865191-
dc.identifier.scopuseid_2-s2.0-0029840752en_HK
dc.identifier.hkuros26053-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0029840752&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume149en_HK
dc.identifier.issue4en_HK
dc.identifier.spage1111en_HK
dc.identifier.epage1118en_HK
dc.identifier.isiWOS:A1996VM31400005-
dc.publisher.placeUnited Statesen_HK

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