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Article: Lysozyme and RNases as anti-HIV components in β-core preparations of human chorionic gonadotropin

TitleLysozyme and RNases as anti-HIV components in β-core preparations of human chorionic gonadotropin
Authors
KeywordsAIDS
Muramidase
Urinary proteins
Antiviral agents
Issue Date1999
PublisherNational Academy of Sciences. The Journal's web site is located at http://www.pnas.org
Citation
National Academy of Sciences Proceedings, 1999, v. 96 n. 6, p. 2678-2681 How to Cite?
AbstractHuman chorionic gonadotropin (hCG) preparations contain activity against HIV type 1 (HIV-1). However, there has been controversy about whether some biological activities of hCG beta-subunit (hCGbeta) preparations are caused by the beta-subunit itself or other proteins present in the preparations. We report here the purification, characterization, and identification of three enzymes with anti-HIV activity present in the beta-core fraction of hCGbeta prepared from the urine of pregnant women. The N-terminal amino acid sequence of one protein is identical to human urinary lysozyme C, and those of the other two are identical to human RNase A and urinary RNase U. We thus refer to these proteins as AVL (antiviral lysozyme) and AVR (antiviral RNases). In addition to HIV-1 inhibition, AVL is capable of lysing Micrococcus lysodeikticus. AVR digests a variety of RNA substrates, including RNA from HIV-1-infected cells. We also find that lysozyme from chicken egg white, human milk, and human neutrophils and RNase A from bovine pancreas possess activity against HIV-1. These findings may offer additional strategies for the treatment of HIV-1 infection.
Persistent Identifierhttp://hdl.handle.net/10722/49400
ISSN
2015 Impact Factor: 9.423
2015 SCImago Journal Rankings: 6.883
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLee-Huang, Sen_HK
dc.contributor.authorHuang, PLen_HK
dc.contributor.authorSun, Yen_HK
dc.contributor.authorHuang, PLen_HK
dc.contributor.authorKung, Hen_HK
dc.contributor.authorBlithe, DLen_HK
dc.contributor.authorChen, HCen_HK
dc.date.accessioned2008-06-12T06:41:34Z-
dc.date.available2008-06-12T06:41:34Z-
dc.date.issued1999en_HK
dc.identifier.citationNational Academy of Sciences Proceedings, 1999, v. 96 n. 6, p. 2678-2681en_HK
dc.identifier.issn0027-8424en_HK
dc.identifier.urihttp://hdl.handle.net/10722/49400-
dc.description.abstractHuman chorionic gonadotropin (hCG) preparations contain activity against HIV type 1 (HIV-1). However, there has been controversy about whether some biological activities of hCG beta-subunit (hCGbeta) preparations are caused by the beta-subunit itself or other proteins present in the preparations. We report here the purification, characterization, and identification of three enzymes with anti-HIV activity present in the beta-core fraction of hCGbeta prepared from the urine of pregnant women. The N-terminal amino acid sequence of one protein is identical to human urinary lysozyme C, and those of the other two are identical to human RNase A and urinary RNase U. We thus refer to these proteins as AVL (antiviral lysozyme) and AVR (antiviral RNases). In addition to HIV-1 inhibition, AVL is capable of lysing Micrococcus lysodeikticus. AVR digests a variety of RNA substrates, including RNA from HIV-1-infected cells. We also find that lysozyme from chicken egg white, human milk, and human neutrophils and RNase A from bovine pancreas possess activity against HIV-1. These findings may offer additional strategies for the treatment of HIV-1 infection.en_HK
dc.format.extent384 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherNational Academy of Sciences. The Journal's web site is located at http://www.pnas.orgen_HK
dc.rightsNational Academy of Sciences Proceedings. Copyright © National Academy of Sciences.en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectAIDSen_HK
dc.subjectMuramidaseen_HK
dc.subjectUrinary proteinsen_HK
dc.subjectAntiviral agentsen_HK
dc.titleLysozyme and RNases as anti-HIV components in β-core preparations of human chorionic gonadotropinen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0027-8424&volume=96&issue=6&spage=2678&epage=2681&date=1999&atitle=Lysozyme+and+RNases+as+anti-HIV+components+in+β-core+preparations+of+human+chorionic+gonadotropinen_HK
dc.identifier.emailKung, H: hkung@hkucc.hku.hken_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1073/pnas.96.6.2678-
dc.identifier.pmid10077570-
dc.identifier.pmcidPMC15828en_HK
dc.identifier.scopuseid_2-s2.0-0033013458-
dc.identifier.isiWOS:000079224500024-

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