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Article: Involvement of aldose reductase in naphthalene cataract

TitleInvolvement of aldose reductase in naphthalene cataract
Authors
Issue Date1998
PublisherAssociation for Research in Vision and Ophthalmology. The Journal's web site is located at http://www.iovs.org
Citation
Investigative Ophthalmology And Visual Science, 1998, v. 39 n. 1, p. 193-197 How to Cite?
AbstractPURPOSE. The authors investigated the involvement of the enzyme aldose reductase (AR) in naphthalene cataract by using transgenic mice that overexpress AR. METHODS. Lenses from nontransgenic mice and from transgenic lines CAR222 and CAR648, with different levels of AR, were cultured in medium containing naphthalene-1,2-diydrodiol (ND) with or without AR inhibitor AL1576. The morphology and progression rate of ND-induced cataract in these lenses were compared. RESULTS. Lenses from transgenic mice CAR222 and CAR648, but not their nontransgenic littermates, developed yellow pigment in the inner cortex when exposed to 50 μM ND, which was completely prevented by 0.2 mM AL1576. The yellow pigment developed faster and more intensely in the CAR648 lens, which has a higher AR level than CAR222. Under a high dose of 500 μM ND, both transgenic and wild-type mouse lenses developed ND-induced cataract, although the first sign of cataract was found in the outer cortex in transgenic lenses instead of the inner cortical region in wild-type lenses. In addition, the cataract was more severe and developed at a faster rate in transgenic mouse lenses. AL1576 showed only partial protection against the cataract induced by 500 μM ND. CONCLUSIONS. The findings showed that the progression rate of ND-induced cataract correlated with the level of lens AR and ND, indicating that AR was the key enzyme for the metabolism of ND in the process of naphthalene cataract development.
Persistent Identifierhttp://hdl.handle.net/10722/49399
ISSN
2015 Impact Factor: 3.427
2015 SCImago Journal Rankings: 2.008
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLee, AYWen_HK
dc.contributor.authorChung, SSMen_HK
dc.date.accessioned2008-06-12T06:41:33Z-
dc.date.available2008-06-12T06:41:33Z-
dc.date.issued1998en_HK
dc.identifier.citationInvestigative Ophthalmology And Visual Science, 1998, v. 39 n. 1, p. 193-197en_HK
dc.identifier.issn0146-0404en_HK
dc.identifier.urihttp://hdl.handle.net/10722/49399-
dc.description.abstractPURPOSE. The authors investigated the involvement of the enzyme aldose reductase (AR) in naphthalene cataract by using transgenic mice that overexpress AR. METHODS. Lenses from nontransgenic mice and from transgenic lines CAR222 and CAR648, with different levels of AR, were cultured in medium containing naphthalene-1,2-diydrodiol (ND) with or without AR inhibitor AL1576. The morphology and progression rate of ND-induced cataract in these lenses were compared. RESULTS. Lenses from transgenic mice CAR222 and CAR648, but not their nontransgenic littermates, developed yellow pigment in the inner cortex when exposed to 50 μM ND, which was completely prevented by 0.2 mM AL1576. The yellow pigment developed faster and more intensely in the CAR648 lens, which has a higher AR level than CAR222. Under a high dose of 500 μM ND, both transgenic and wild-type mouse lenses developed ND-induced cataract, although the first sign of cataract was found in the outer cortex in transgenic lenses instead of the inner cortical region in wild-type lenses. In addition, the cataract was more severe and developed at a faster rate in transgenic mouse lenses. AL1576 showed only partial protection against the cataract induced by 500 μM ND. CONCLUSIONS. The findings showed that the progression rate of ND-induced cataract correlated with the level of lens AR and ND, indicating that AR was the key enzyme for the metabolism of ND in the process of naphthalene cataract development.en_HK
dc.format.extent418 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherAssociation for Research in Vision and Ophthalmology. The Journal's web site is located at http://www.iovs.orgen_HK
dc.relation.ispartofInvestigative Ophthalmology and Visual Scienceen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshAldehyde Reductase - antagonists & inhibitors - genetics - metabolismen_HK
dc.subject.meshCataract - chemically induced - enzymology - pathologyen_HK
dc.subject.meshLens, Crystalline - drug effects - enzymology - pathologyen_HK
dc.subject.meshNaphthols - toxicityen_HK
dc.subject.meshCulture Mediaen_HK
dc.titleInvolvement of aldose reductase in naphthalene cataracten_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0146-0404&volume=39&issue=1&spage=193&epage=197&date=1998&atitle=Involvement+of+aldose+reductase+in+naphthalene+cataracten_HK
dc.identifier.emailChung, SSM: smchung@hkucc.hku.hken_HK
dc.identifier.authorityChung, SSM=rp00376en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.pmid9430562-
dc.identifier.scopuseid_2-s2.0-0031964665en_HK
dc.identifier.hkuros36088-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031964665&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume39en_HK
dc.identifier.issue1en_HK
dc.identifier.spage193en_HK
dc.identifier.epage197en_HK
dc.identifier.isiWOS:000071410800024-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLee, AYW=55477259800en_HK
dc.identifier.scopusauthoridChung, SSM=14120761600en_HK

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