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Article: Partial purification of embryotrophic factors from human oviductal cells

TitlePartial purification of embryotrophic factors from human oviductal cells
Authors
KeywordsCo-culture
Embryos
Embryotrophic factor
Glycoprotein
Oviductal cells
Issue Date1998
PublisherOxford University Press. The Journal's web site is located at http://humrep.oxfordjournals.org/
Citation
Human Reproduction, 1998, v. 13 n. 6, p. 1613-1619 How to Cite?
AbstractEmbryotrophic factors from human oviductal cells were partially purified by liquid chromatographic methods. The conditioned medium from human oviductal cell culture was fractionated successively by concanavalin A (Con-A) affinity chromatography, ion-exchange chromatography and gel filtration. The presence of the embryotrophic activity in the eluates was determined by the stimulatory effects on the development of mouse embryos in vitro. The fraction that did not bind to the lectin Con-A possessed no embryotrophic activity. Ion-exchange chromatography separated the glycoproteins that bound to Con-A into five fractions. Three of them significantly enhanced blastulation as well as conceptus formation. Gel filtration further separated these embryotrophic fractions into five fractions. Three of them with molecular weights of 154 ± 1, 164 ± 0.2 and 207 ± 0.3 kDa significantly stimulated blastulation of mouse embryos. The results of this study demonstrated that several embryotrophic factors with different biochemical properties contributed to the embryotrophic effect of the human oviductal cell/mouse embryo co-culture system.
Persistent Identifierhttp://hdl.handle.net/10722/49389
ISSN
2015 Impact Factor: 4.621
2015 SCImago Journal Rankings: 2.271
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLiu, LPSen_HK
dc.contributor.authorChan, STHen_HK
dc.contributor.authorHo, PCen_HK
dc.contributor.authorYeung, WSBen_HK
dc.date.accessioned2008-06-12T06:41:09Z-
dc.date.available2008-06-12T06:41:09Z-
dc.date.issued1998en_HK
dc.identifier.citationHuman Reproduction, 1998, v. 13 n. 6, p. 1613-1619en_HK
dc.identifier.issn0268-1161en_HK
dc.identifier.urihttp://hdl.handle.net/10722/49389-
dc.description.abstractEmbryotrophic factors from human oviductal cells were partially purified by liquid chromatographic methods. The conditioned medium from human oviductal cell culture was fractionated successively by concanavalin A (Con-A) affinity chromatography, ion-exchange chromatography and gel filtration. The presence of the embryotrophic activity in the eluates was determined by the stimulatory effects on the development of mouse embryos in vitro. The fraction that did not bind to the lectin Con-A possessed no embryotrophic activity. Ion-exchange chromatography separated the glycoproteins that bound to Con-A into five fractions. Three of them significantly enhanced blastulation as well as conceptus formation. Gel filtration further separated these embryotrophic fractions into five fractions. Three of them with molecular weights of 154 ± 1, 164 ± 0.2 and 207 ± 0.3 kDa significantly stimulated blastulation of mouse embryos. The results of this study demonstrated that several embryotrophic factors with different biochemical properties contributed to the embryotrophic effect of the human oviductal cell/mouse embryo co-culture system.en_HK
dc.format.extent418 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherOxford University Press. The Journal's web site is located at http://humrep.oxfordjournals.org/en_HK
dc.relation.ispartofHuman Reproductionen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectCo-cultureen_HK
dc.subjectEmbryosen_HK
dc.subjectEmbryotrophic factoren_HK
dc.subjectGlycoproteinen_HK
dc.subjectOviductal cellsen_HK
dc.titlePartial purification of embryotrophic factors from human oviductal cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0268-1161&volume=13&issue=6&spage=1613&epage=1619&date=1998&atitle=Partial+purification+of+embryotrophic+factors+from+human+oviductal+cellsen_HK
dc.identifier.emailHo, PC:pcho@hku.hken_HK
dc.identifier.emailYeung, WSB:wsbyeung@hkucc.hku.hken_HK
dc.identifier.authorityHo, PC=rp00325en_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1093/humrep/13.6.1613en_HK
dc.identifier.pmid9688401-
dc.identifier.scopuseid_2-s2.0-0031861185en_HK
dc.identifier.hkuros39259-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031861185&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume13en_HK
dc.identifier.issue6en_HK
dc.identifier.spage1613en_HK
dc.identifier.epage1619en_HK
dc.identifier.isiWOS:000076030800041-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLiu, LPS=19335129300en_HK
dc.identifier.scopusauthoridChan, STH=24368283200en_HK
dc.identifier.scopusauthoridHo, PC=7402211440en_HK
dc.identifier.scopusauthoridYeung, WSB=7102370745en_HK

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