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Article: The effects of follicular fluid from patients with different indications for IVF treatment on the binding of human spermatozoa to the zona pellucida

TitleThe effects of follicular fluid from patients with different indications for IVF treatment on the binding of human spermatozoa to the zona pellucida
Authors
KeywordsEndometriosis
Follicular fluid
Hemizona binding assay
Male factor
Tubal factor
Issue Date1998
PublisherOxford University Press. The Journal's web site is located at http://humrep.oxfordjournals.org/
Citation
Human Reproduction, 1998, v. 13 n. 1, p. 128-131 How to Cite?
AbstractThis study compared the effects of human follicular fluid (hFF) from women with endometriosis, tubal factor and male factor on the zona binding capacity of human spermatozoa. Samples of hFF were collected from 30 patients, 10 patients for each of the indications of infertility, at the time of oocyte retrieval in an in-vitro fertilization/embryo transfer programme. The hemizona binding assay (HZA) was used to assess the effect of these hFF on the zona binding potential of human spermatozoa. The mean numbers of spermatozoa bound to the zona pellucida after treating the spermatozoa with hFF from endometriosis, tubal factor and male factor were 90.5 ± 20.9, 108.9 ± 22.3 and 101.2 ± 13.4 respectively. These were significantly lower than their corresponding controls, the spermatozoa of which were incubated with Earle's balanced salt solution (endometriosis 238.7 ± 34.7; tubal factor 210.8 ± 41.6; male factor 205.4 ± 26.3; P < 0.002). The hemizona binding index (HZI) was similar between male factor samples (52.0 ± 6.7) and tubal factor samples (53.8 ± 4.2). Spermatozoa incubated with hFF from endometriosis patients (36.0 ± 4.1) had an HZI that was significantly lower than those treated with hFF from tubal factor patients (P < 0.01). Probably due to small sample size, the differences in HZI between endometriosis samples and male factor samples did not reach statistical significance (P = 0.076). These data suggest that there was a stronger sperm-zona binding inhibitory effect of hFF from patients with endometriosis than from those without the disease.
Persistent Identifierhttp://hdl.handle.net/10722/49386
ISSN
2015 Impact Factor: 4.621
2015 SCImago Journal Rankings: 2.271
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorQiao, Jen_HK
dc.contributor.authorYeung, WSBen_HK
dc.contributor.authorYao, YQen_HK
dc.contributor.authorHo, PCen_HK
dc.date.accessioned2008-06-12T06:41:05Z-
dc.date.available2008-06-12T06:41:05Z-
dc.date.issued1998en_HK
dc.identifier.citationHuman Reproduction, 1998, v. 13 n. 1, p. 128-131en_HK
dc.identifier.issn0268-1161en_HK
dc.identifier.urihttp://hdl.handle.net/10722/49386-
dc.description.abstractThis study compared the effects of human follicular fluid (hFF) from women with endometriosis, tubal factor and male factor on the zona binding capacity of human spermatozoa. Samples of hFF were collected from 30 patients, 10 patients for each of the indications of infertility, at the time of oocyte retrieval in an in-vitro fertilization/embryo transfer programme. The hemizona binding assay (HZA) was used to assess the effect of these hFF on the zona binding potential of human spermatozoa. The mean numbers of spermatozoa bound to the zona pellucida after treating the spermatozoa with hFF from endometriosis, tubal factor and male factor were 90.5 ± 20.9, 108.9 ± 22.3 and 101.2 ± 13.4 respectively. These were significantly lower than their corresponding controls, the spermatozoa of which were incubated with Earle's balanced salt solution (endometriosis 238.7 ± 34.7; tubal factor 210.8 ± 41.6; male factor 205.4 ± 26.3; P < 0.002). The hemizona binding index (HZI) was similar between male factor samples (52.0 ± 6.7) and tubal factor samples (53.8 ± 4.2). Spermatozoa incubated with hFF from endometriosis patients (36.0 ± 4.1) had an HZI that was significantly lower than those treated with hFF from tubal factor patients (P < 0.01). Probably due to small sample size, the differences in HZI between endometriosis samples and male factor samples did not reach statistical significance (P = 0.076). These data suggest that there was a stronger sperm-zona binding inhibitory effect of hFF from patients with endometriosis than from those without the disease.en_HK
dc.format.extent418 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherOxford University Press. The Journal's web site is located at http://humrep.oxfordjournals.org/en_HK
dc.relation.ispartofHuman Reproductionen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectEndometriosisen_HK
dc.subjectFollicular fluiden_HK
dc.subjectHemizona binding assayen_HK
dc.subjectMale factoren_HK
dc.subjectTubal factoren_HK
dc.titleThe effects of follicular fluid from patients with different indications for IVF treatment on the binding of human spermatozoa to the zona pellucidaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0268-1161&volume=13&issue=1&spage=128&epage=131&date=1998&atitle=The+effects+of+follicular+fluid+from+patients+with+different+indications+for+IVF+treatment+on+the+binding+of+human+spermatozoa+to+the+zona+pellucidaen_HK
dc.identifier.emailYeung, WSB:wsbyeung@hkucc.hku.hken_HK
dc.identifier.emailHo, PC:pcho@hku.hken_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.identifier.authorityHo, PC=rp00325en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1093/humrep/13.1.128en_HK
dc.identifier.pmid9512243-
dc.identifier.scopuseid_2-s2.0-0031931752en_HK
dc.identifier.hkuros31663-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031931752&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume13en_HK
dc.identifier.issue1en_HK
dc.identifier.spage128en_HK
dc.identifier.epage131en_HK
dc.identifier.isiWOS:000072396100028-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridQiao, J=16025742900en_HK
dc.identifier.scopusauthoridYeung, WSB=7102370745en_HK
dc.identifier.scopusauthoridYao, YQ=7403567431en_HK
dc.identifier.scopusauthoridHo, PC=7402211440en_HK

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