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Article: The factors affecting sperm binding to the zona pellucida in the hemizona binding assay

TitleThe factors affecting sperm binding to the zona pellucida in the hemizona binding assay
Authors
KeywordsHemizona binding assay
Medium
Protein
Spermatozoa
Zona pellucida
Issue Date1996
PublisherOxford University Press. The Journal's web site is located at http://humrep.oxfordjournals.org/
Citation
Human Reproduction, 1996, v. 11 n. 7, p. 1516-1519 How to Cite?
AbstractSperm binding to the zona pellucida is a prerequisite for fertilization. The hemizona binding assay (HZA) is commonly used to evaluate the zona-binding capacity of spermatozoa. The present study reports three factors that affect HZA. They were the base medium used, the protein source and the size of pipette used for removing loosely bound spermatozoa during HZA. The number of spermatozoa bound on the hemizona was compared between (1) Earle's balanced salt solution (EBSS) and Ham's F-10, and (2) between human serum and bovine serum albumin (BSA). Results indicated that EBSS and human serum both significantly increase the number of bound spermatozoa when compared to Ham's F-10 (P < 0.0001, paired t-test) and BSA (P < 0.05, paired t-test) respectively. Pipettes of different diameters were used to study the effect of size in removing loosely bound spermatozoa on hemizona. Data showed that the diameter of the pipette should be ≤ 200 mm, in order not to remove bound spermatozoa excessively. These results emphasize the importance of standardization of the protocol of the hemizona assay worldwide to be able to compare results between different laboratories.
Persistent Identifierhttp://hdl.handle.net/10722/49383
ISSN
2015 Impact Factor: 4.621
2015 SCImago Journal Rankings: 2.271
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYao, YQen_HK
dc.contributor.authorYeung, WSBen_HK
dc.contributor.authorHo, PCen_HK
dc.date.accessioned2008-06-12T06:41:01Z-
dc.date.available2008-06-12T06:41:01Z-
dc.date.issued1996en_HK
dc.identifier.citationHuman Reproduction, 1996, v. 11 n. 7, p. 1516-1519en_HK
dc.identifier.issn0268-1161en_HK
dc.identifier.urihttp://hdl.handle.net/10722/49383-
dc.description.abstractSperm binding to the zona pellucida is a prerequisite for fertilization. The hemizona binding assay (HZA) is commonly used to evaluate the zona-binding capacity of spermatozoa. The present study reports three factors that affect HZA. They were the base medium used, the protein source and the size of pipette used for removing loosely bound spermatozoa during HZA. The number of spermatozoa bound on the hemizona was compared between (1) Earle's balanced salt solution (EBSS) and Ham's F-10, and (2) between human serum and bovine serum albumin (BSA). Results indicated that EBSS and human serum both significantly increase the number of bound spermatozoa when compared to Ham's F-10 (P < 0.0001, paired t-test) and BSA (P < 0.05, paired t-test) respectively. Pipettes of different diameters were used to study the effect of size in removing loosely bound spermatozoa on hemizona. Data showed that the diameter of the pipette should be ≤ 200 mm, in order not to remove bound spermatozoa excessively. These results emphasize the importance of standardization of the protocol of the hemizona assay worldwide to be able to compare results between different laboratories.en_HK
dc.format.extent418 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherOxford University Press. The Journal's web site is located at http://humrep.oxfordjournals.org/en_HK
dc.relation.ispartofHuman Reproductionen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectHemizona binding assayen_HK
dc.subjectMediumen_HK
dc.subjectProteinen_HK
dc.subjectSpermatozoaen_HK
dc.subjectZona pellucidaen_HK
dc.titleThe factors affecting sperm binding to the zona pellucida in the hemizona binding assayen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0268-1161&volume=11&issue=7&spage=1516&epage=1519&date=1996&atitle=The+factors+affecting+sperm+binding+to+the+zona+pellucida+in+the+hemizona+binding+assayen_HK
dc.identifier.emailYeung, WSB:wsbyeung@hkucc.hku.hken_HK
dc.identifier.emailHo, PC:pcho@hku.hken_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.identifier.authorityHo, PC=rp00325en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.pmid8671496-
dc.identifier.scopuseid_2-s2.0-0029810035en_HK
dc.identifier.hkuros23020-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0029810035&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume11en_HK
dc.identifier.issue7en_HK
dc.identifier.spage1516en_HK
dc.identifier.epage1519en_HK
dc.identifier.isiWOS:A1996VD88000035-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridYao, YQ=7403567431en_HK
dc.identifier.scopusauthoridYeung, WSB=7102370745en_HK
dc.identifier.scopusauthoridHo, PC=7402211440en_HK

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