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Article: Human follicular fluid inhibits the binding of human spermatozoa to zona pellucida in vitro

TitleHuman follicular fluid inhibits the binding of human spermatozoa to zona pellucida in vitro
Authors
KeywordsAcrosome reaction
Follicular fluid
Hemizona binding assay
Motility
Progesterone
Issue Date1996
PublisherOxford University Press. The Journal's web site is located at http://humrep.oxfordjournals.org/
Citation
Human Reproduction, 1996, v. 11 n. 12, p. 2674-2680 How to Cite?
AbstractThe effect of human follicular fluid on human zona pellucida binding of spermatozoa was investigated using the hemizona binding assay (HZA). This effect was compared to that of progesterone, a known component of human follicular fluid. Exposure of spermatozoa to 25% pooled human follicular fluid for 1 h significantly reduced the number of spermatozoa bound to zona pellucida when compared to those without human follicular fluid treatment (149.1 ± 30.7 versus 177.1 ± 33.8, P < 0.01). The same phenomenon was observed after 3 h of treatment. The corresponding numbers of bound spermatozoa were 140.4 ± 19.1 and 200.2 ± 23.4 (P < 0.0001). Progesterone (1.0 μg/ml) stimulated the zona pellucida-binding capacity of spermatozoa significantly under the same conditions (P < 0.01). The numbers of bound spermatozoa after 1 and 3 h progesterone treatment were 235.5 ± 44.7 (control, 168.1 ± 32.9) and 204.3 ± 27.4 (control, 162.3 ± 20.1) respectively. HZA comparing the effects of human follicular fluid and progesterone at concentrations equivalent to those found in human follicular fluid using matching hemizonae confirmed the inhibitory effect of human follicular fluid on sperm binding to zona pellucida (80.4 ± 28.4 versus 149.8 ± 35.2, P < 0.05). This inhibitory effect was also found in another eight individual human follicular fluid samples. Both human follicular fluid and progesterone did not affect the motility and viability of the treated spermatozoa when compared to the controls with the same incubation period. Although more spermatozoa underwent the acrosome reaction after 1 and 3 h of human follicular fluid treatment than in the control, the extent was comparable to those after progesterone treatment. These results suggested that human follicular fluid inhibited the zona pellucida-binding capacity of spermatozoa in vitro. This inhibitory effect of human follicular fluid was not mediated by progesterone, and did not result from the effects of human follicular fluid on sperm motility, viability and acrosome reaction.
Persistent Identifierhttp://hdl.handle.net/10722/49382
ISSN
2015 Impact Factor: 4.621
2015 SCImago Journal Rankings: 2.271
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYao, YQen_HK
dc.contributor.authorYeung, WSBen_HK
dc.contributor.authorHo, PCen_HK
dc.date.accessioned2008-06-12T06:40:59Z-
dc.date.available2008-06-12T06:40:59Z-
dc.date.issued1996en_HK
dc.identifier.citationHuman Reproduction, 1996, v. 11 n. 12, p. 2674-2680en_HK
dc.identifier.issn0268-1161en_HK
dc.identifier.urihttp://hdl.handle.net/10722/49382-
dc.description.abstractThe effect of human follicular fluid on human zona pellucida binding of spermatozoa was investigated using the hemizona binding assay (HZA). This effect was compared to that of progesterone, a known component of human follicular fluid. Exposure of spermatozoa to 25% pooled human follicular fluid for 1 h significantly reduced the number of spermatozoa bound to zona pellucida when compared to those without human follicular fluid treatment (149.1 ± 30.7 versus 177.1 ± 33.8, P < 0.01). The same phenomenon was observed after 3 h of treatment. The corresponding numbers of bound spermatozoa were 140.4 ± 19.1 and 200.2 ± 23.4 (P < 0.0001). Progesterone (1.0 μg/ml) stimulated the zona pellucida-binding capacity of spermatozoa significantly under the same conditions (P < 0.01). The numbers of bound spermatozoa after 1 and 3 h progesterone treatment were 235.5 ± 44.7 (control, 168.1 ± 32.9) and 204.3 ± 27.4 (control, 162.3 ± 20.1) respectively. HZA comparing the effects of human follicular fluid and progesterone at concentrations equivalent to those found in human follicular fluid using matching hemizonae confirmed the inhibitory effect of human follicular fluid on sperm binding to zona pellucida (80.4 ± 28.4 versus 149.8 ± 35.2, P < 0.05). This inhibitory effect was also found in another eight individual human follicular fluid samples. Both human follicular fluid and progesterone did not affect the motility and viability of the treated spermatozoa when compared to the controls with the same incubation period. Although more spermatozoa underwent the acrosome reaction after 1 and 3 h of human follicular fluid treatment than in the control, the extent was comparable to those after progesterone treatment. These results suggested that human follicular fluid inhibited the zona pellucida-binding capacity of spermatozoa in vitro. This inhibitory effect of human follicular fluid was not mediated by progesterone, and did not result from the effects of human follicular fluid on sperm motility, viability and acrosome reaction.en_HK
dc.format.extent418 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherOxford University Press. The Journal's web site is located at http://humrep.oxfordjournals.org/en_HK
dc.relation.ispartofHuman Reproductionen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectAcrosome reactionen_HK
dc.subjectFollicular fluiden_HK
dc.subjectHemizona binding assayen_HK
dc.subjectMotilityen_HK
dc.subjectProgesteroneen_HK
dc.titleHuman follicular fluid inhibits the binding of human spermatozoa to zona pellucida in vitroen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0268-1161&volume=11&issue=12&spage=2674&epage=2680&date=1996&atitle=Human+follicular+fluid+inhibits+the+binding+of+human+spermatozoa+to+zona+pellucida+in+vitroen_HK
dc.identifier.emailYeung, WSB:wsbyeung@hkucc.hku.hken_HK
dc.identifier.emailHo, PC:pcho@hku.hken_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.identifier.authorityHo, PC=rp00325en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.pmid9021371-
dc.identifier.scopuseid_2-s2.0-0030466418en_HK
dc.identifier.hkuros21792-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0030466418&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume11en_HK
dc.identifier.issue12en_HK
dc.identifier.spage2674en_HK
dc.identifier.epage2680en_HK
dc.identifier.isiWOS:A1996WE39900023-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridYao, YQ=7403567431en_HK
dc.identifier.scopusauthoridYeung, WSB=7102370745en_HK
dc.identifier.scopusauthoridHo, PC=7402211440en_HK

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