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Article: A YAC-based contig of 1.5 mb spanning the human multidrug resistance gene region and delineating the amplification unit in three human multidrug-resistant cell lines
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TitleA YAC-based contig of 1.5 mb spanning the human multidrug resistance gene region and delineating the amplification unit in three human multidrug-resistant cell lines
 
AuthorsTorigoe, K1
Sato, S1
Kusaba, H1
Kohno, K1
Kuwano, M1
Okumura, K1
Green, ED1
Tsui, LC1
Scherer, SW1
Schlessinger, D1
Wada, M1
 
Issue Date1995
 
PublisherCold Spring Harbor Laboratory Press, Publications Department.
 
CitationGenome Research, 1995, v. 5 n. 3, p. 233-244 [How to Cite?]
DOI: http://dx.doi.org/10.1101/gr.5.3.233
 
AbstractA contig of 21 nonchimeric yeast artificial chromosomes (YACs) has been assembled across 1.5 Mb of the multidrug resistance (MDR) gene region located at 7q21, and formatted with four previously reported probes, six newly isolated probes, and three sequence-tagged sites (STSs) from internal and end fragments of YACs. A physical map of rare cutter restriction enzyme sites across the region was also constructed by pulsed-field gel electrophoretic (PFGE) analysis of four overlapping YAC clones. The amplification unit of this region in different cell lines was then determined by Southern blot analysis on the basis of the physical map and probes. Amplified DNA was located in extrachromosomal elements in human MDR cell lines studied here, and the size of the amplification unit was determined to be discrete in one MDR amplification but variable in others.
 
ISSN1054-9803
 
DOIhttp://dx.doi.org/10.1101/gr.5.3.233
 
ISI Accession Number IDWOS:A1995TF34800004
 
DC FieldValue
dc.contributor.authorTorigoe, K
 
dc.contributor.authorSato, S
 
dc.contributor.authorKusaba, H
 
dc.contributor.authorKohno, K
 
dc.contributor.authorKuwano, M
 
dc.contributor.authorOkumura, K
 
dc.contributor.authorGreen, ED
 
dc.contributor.authorTsui, LC
 
dc.contributor.authorScherer, SW
 
dc.contributor.authorSchlessinger, D
 
dc.contributor.authorWada, M
 
dc.date.accessioned2008-06-12T06:40:28Z
 
dc.date.available2008-06-12T06:40:28Z
 
dc.date.issued1995
 
dc.description.abstractA contig of 21 nonchimeric yeast artificial chromosomes (YACs) has been assembled across 1.5 Mb of the multidrug resistance (MDR) gene region located at 7q21, and formatted with four previously reported probes, six newly isolated probes, and three sequence-tagged sites (STSs) from internal and end fragments of YACs. A physical map of rare cutter restriction enzyme sites across the region was also constructed by pulsed-field gel electrophoretic (PFGE) analysis of four overlapping YAC clones. The amplification unit of this region in different cell lines was then determined by Southern blot analysis on the basis of the physical map and probes. Amplified DNA was located in extrachromosomal elements in human MDR cell lines studied here, and the size of the amplification unit was determined to be discrete in one MDR amplification but variable in others.
 
dc.description.naturepublished_or_final_version
 
dc.format.extent418 bytes
 
dc.format.mimetypetext/html
 
dc.identifier.citationGenome Research, 1995, v. 5 n. 3, p. 233-244 [How to Cite?]
DOI: http://dx.doi.org/10.1101/gr.5.3.233
 
dc.identifier.doihttp://dx.doi.org/10.1101/gr.5.3.233
 
dc.identifier.epage244
 
dc.identifier.isiWOS:A1995TF34800004
 
dc.identifier.issn1054-9803
 
dc.identifier.issue3
 
dc.identifier.openurl
 
dc.identifier.pmid8593611
 
dc.identifier.scopuseid_2-s2.0-0028784609
 
dc.identifier.spage233
 
dc.identifier.urihttp://hdl.handle.net/10722/49363
 
dc.identifier.volume5
 
dc.languageeng
 
dc.publisherCold Spring Harbor Laboratory Press, Publications Department.
 
dc.relation.ispartofGenome Research
 
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
 
dc.subject.meshChromosomes, Artificial, Yeast
 
dc.subject.meshChromosomes, Human, Pair 7 - genetics
 
dc.subject.meshDrug Resistance, Multiple - genetics
 
dc.subject.meshP-Glycoproteins
 
dc.subject.meshSequence Tagged Sites
 
dc.titleA YAC-based contig of 1.5 mb spanning the human multidrug resistance gene region and delineating the amplification unit in three human multidrug-resistant cell lines
 
dc.typeArticle
 
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Author Affiliations
  1. Kyushu University Faculty of Medicine