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Article: A YAC-based contig of 1.5 mb spanning the human multidrug resistance gene region and delineating the amplification unit in three human multidrug-resistant cell lines

TitleA YAC-based contig of 1.5 mb spanning the human multidrug resistance gene region and delineating the amplification unit in three human multidrug-resistant cell lines
Authors
Issue Date1995
PublisherCold Spring Harbor Laboratory Press, Publications Department.
Citation
Genome Research, 1995, v. 5 n. 3, p. 233-244 How to Cite?
Abstract
A contig of 21 nonchimeric yeast artificial chromosomes (YACs) has been assembled across 1.5 Mb of the multidrug resistance (MDR) gene region located at 7q21, and formatted with four previously reported probes, six newly isolated probes, and three sequence-tagged sites (STSs) from internal and end fragments of YACs. A physical map of rare cutter restriction enzyme sites across the region was also constructed by pulsed-field gel electrophoretic (PFGE) analysis of four overlapping YAC clones. The amplification unit of this region in different cell lines was then determined by Southern blot analysis on the basis of the physical map and probes. Amplified DNA was located in extrachromosomal elements in human MDR cell lines studied here, and the size of the amplification unit was determined to be discrete in one MDR amplification but variable in others.
Persistent Identifierhttp://hdl.handle.net/10722/49363
ISSN
ISI Accession Number ID

 

Author Affiliations
  1. Kyushu University Faculty of Medicine
DC FieldValueLanguage
dc.contributor.authorTorigoe, Ken_HK
dc.contributor.authorSato, Sen_HK
dc.contributor.authorKusaba, Hen_HK
dc.contributor.authorKohno, Ken_HK
dc.contributor.authorKuwano, Men_HK
dc.contributor.authorOkumura, Ken_HK
dc.contributor.authorGreen, EDen_HK
dc.contributor.authorTsui, LCen_HK
dc.contributor.authorScherer, SWen_HK
dc.contributor.authorSchlessinger, Den_HK
dc.contributor.authorWada, Men_HK
dc.date.accessioned2008-06-12T06:40:28Z-
dc.date.available2008-06-12T06:40:28Z-
dc.date.issued1995en_HK
dc.identifier.citationGenome Research, 1995, v. 5 n. 3, p. 233-244en_HK
dc.identifier.issn1054-9803en_HK
dc.identifier.urihttp://hdl.handle.net/10722/49363-
dc.description.abstractA contig of 21 nonchimeric yeast artificial chromosomes (YACs) has been assembled across 1.5 Mb of the multidrug resistance (MDR) gene region located at 7q21, and formatted with four previously reported probes, six newly isolated probes, and three sequence-tagged sites (STSs) from internal and end fragments of YACs. A physical map of rare cutter restriction enzyme sites across the region was also constructed by pulsed-field gel electrophoretic (PFGE) analysis of four overlapping YAC clones. The amplification unit of this region in different cell lines was then determined by Southern blot analysis on the basis of the physical map and probes. Amplified DNA was located in extrachromosomal elements in human MDR cell lines studied here, and the size of the amplification unit was determined to be discrete in one MDR amplification but variable in others.en_HK
dc.format.extent418 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherCold Spring Harbor Laboratory Press, Publications Department.en_HK
dc.relation.ispartofGenome Researchen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshChromosomes, Artificial, Yeasten_HK
dc.subject.meshChromosomes, Human, Pair 7 - geneticsen_HK
dc.subject.meshDrug Resistance, Multiple - geneticsen_HK
dc.subject.meshP-Glycoproteinsen_HK
dc.subject.meshSequence Tagged Sitesen_HK
dc.titleA YAC-based contig of 1.5 mb spanning the human multidrug resistance gene region and delineating the amplification unit in three human multidrug-resistant cell linesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1088-9051&volume=5&issue=3&spage=233&epage=244&date=1995&atitle=A+YAC-based+contig+of+1.5+Mb+spanning+the+human+multidrug+resistance+gene+region+and+delineating+the+amplification+unit+in+three+human+multidrug-resistant+cell+linesen_HK
dc.identifier.emailTsui, LC: tsuilc@hkucc.hku.hken_HK
dc.identifier.authorityTsui, LC=rp00058en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1101/gr.5.3.233en_HK
dc.identifier.pmid8593611en_HK
dc.identifier.scopuseid_2-s2.0-0028784609en_HK
dc.identifier.volume5en_HK
dc.identifier.issue3en_HK
dc.identifier.spage233en_HK
dc.identifier.epage244en_HK
dc.identifier.isiWOS:A1995TF34800004-
dc.identifier.scopusauthoridTorigoe, K=7006588353en_HK
dc.identifier.scopusauthoridSato, S=35393244300en_HK
dc.identifier.scopusauthoridKusaba, H=6701575870en_HK
dc.identifier.scopusauthoridKohno, K=7201467409en_HK
dc.identifier.scopusauthoridKuwano, M=7103200772en_HK
dc.identifier.scopusauthoridOkumura, K=7402768737en_HK
dc.identifier.scopusauthoridGreen, ED=7201576916en_HK
dc.identifier.scopusauthoridTsui, LC=7102754167en_HK
dc.identifier.scopusauthoridScherer, SW=35374654500en_HK
dc.identifier.scopusauthoridSchlessinger, D=7103215816en_HK
dc.identifier.scopusauthoridWada, M=7401843807en_HK

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