Article: A YAC-based contig of 1.5 mb spanning the human multidrug resistance gene region and delineating the amplification unit in three human multidrug-resistant cell lines

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TitleA YAC-based contig of 1.5 mb spanning the human multidrug resistance gene region and delineating the amplification unit in three human multidrug-resistant cell lines
AuthorsTorigoe, K1
Sato, S1
Kusaba, H1
Kohno, K1
Kuwano, M1
Okumura, K1
Green, ED1
Tsui, LC1
Scherer, SW1
Schlessinger, D1
Wada, M1
Issue Date1995
PublisherCold Spring Harbor Laboratory Press, Publications Department.
CitationGenome Research, 1995, v. 5 n. 3, p. 233-244 [How to Cite?]
DOI: http://dx.doi.org/10.1101/gr.5.3.233
AbstractA contig of 21 nonchimeric yeast artificial chromosomes (YACs) has been assembled across 1.5 Mb of the multidrug resistance (MDR) gene region located at 7q21, and formatted with four previously reported probes, six newly isolated probes, and three sequence-tagged sites (STSs) from internal and end fragments of YACs. A physical map of rare cutter restriction enzyme sites across the region was also constructed by pulsed-field gel electrophoretic (PFGE) analysis of four overlapping YAC clones. The amplification unit of this region in different cell lines was then determined by Southern blot analysis on the basis of the physical map and probes. Amplified DNA was located in extrachromosomal elements in human MDR cell lines studied here, and the size of the amplification unit was determined to be discrete in one MDR amplification but variable in others.
ISSN1054-9803
DOIhttp://dx.doi.org/10.1101/gr.5.3.233
ISI Accession Number IDWOS:A1995TF34800004
DC Field
Value
dc.contributor.authorTorigoe, K
dc.contributor.authorSato, S
dc.contributor.authorKusaba, H
dc.contributor.authorKohno, K
dc.contributor.authorKuwano, M
dc.contributor.authorOkumura, K
dc.contributor.authorGreen, ED
dc.contributor.authorTsui, LC
dc.contributor.authorScherer, SW
dc.contributor.authorSchlessinger, D
dc.contributor.authorWada, M
dc.date.accessioned2008-06-12T06:40:28Z
dc.date.available2008-06-12T06:40:28Z
dc.date.issued1995
dc.description.abstractA contig of 21 nonchimeric yeast artificial chromosomes (YACs) has been assembled across 1.5 Mb of the multidrug resistance (MDR) gene region located at 7q21, and formatted with four previously reported probes, six newly isolated probes, and three sequence-tagged sites (STSs) from internal and end fragments of YACs. A physical map of rare cutter restriction enzyme sites across the region was also constructed by pulsed-field gel electrophoretic (PFGE) analysis of four overlapping YAC clones. The amplification unit of this region in different cell lines was then determined by Southern blot analysis on the basis of the physical map and probes. Amplified DNA was located in extrachromosomal elements in human MDR cell lines studied here, and the size of the amplification unit was determined to be discrete in one MDR amplification but variable in others.
dc.description.naturepublished_or_final_version
dc.format.extent418 bytes
dc.format.mimetypetext/html
dc.identifier.citationGenome Research, 1995, v. 5 n. 3, p. 233-244 [How to Cite?]
DOI: http://dx.doi.org/10.1101/gr.5.3.233
dc.identifier.doihttp://dx.doi.org/10.1101/gr.5.3.233
dc.identifier.epage244
dc.identifier.isiWOS:A1995TF34800004
dc.identifier.issn1054-9803
dc.identifier.issue3
dc.identifier.openurl
dc.identifier.pmid8593611
dc.identifier.scopuseid_2-s2.0-0028784609
dc.identifier.spage233
dc.identifier.urihttp://hdl.handle.net/10722/49363
dc.identifier.volume5
dc.languageeng
dc.publisherCold Spring Harbor Laboratory Press, Publications Department.
dc.relation.ispartofGenome Research
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
dc.subject.meshChromosomes, Artificial, Yeast
dc.subject.meshChromosomes, Human, Pair 7 - genetics
dc.subject.meshDrug Resistance, Multiple - genetics
dc.subject.meshP-Glycoproteins
dc.subject.meshSequence Tagged Sites
dc.titleA YAC-based contig of 1.5 mb spanning the human multidrug resistance gene region and delineating the amplification unit in three human multidrug-resistant cell lines
dc.typeArticle
Author Affiliations
  1. Kyushu University Faculty of Medicine