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Article: Regulation of anion secretion by cyclo-oxygenase and prostanoids in cultured epididymal epithelia from the rat

TitleRegulation of anion secretion by cyclo-oxygenase and prostanoids in cultured epididymal epithelia from the rat
Authors
Issue Date1999
PublisherWiley-Blackwell Publishing Ltd.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0022-3751
Citation
Journal Of Physiology, 1999, v. 514 n. 3, p. 809-820 How to Cite?
Abstract1. The role of cyclo-oxygenase (COX) in the regulation of anion secretion (measured as short-circuit current, I(SC)) in cultured epididymal epithelia from immature rats was investigated. 2. COX inhibitors attenuated the increase of anion secretion caused by bradykinin (LBK) but had no effect on that caused by PGE 2, suggesting that prostaglandin synthesis mediates the secretory response of the tissues to LBK. 3. The apparent IC 50 values for indomethacin, piroxicam and L-745,337 in inhibiting the LBK-induced I(SC) were 0.14, 1.34 and 15.7 μM, respectively. This order of potency: indomethacin > piroxicam > L-745,337 >> DFU suggests the involvement of the COX-1 isozyme in the mediation of the secretory response to LBK. 4. Among the COX products (prostaglandins, thromboxane and prostacyclins) tested, only PGE 2 and, to a much lesser extent, PGF(2α) stimulated anion secretion by cultured rat epididymal epithelia. 5. The effect of PGE 2 was mimicked by 11-deoxyl PGE 1, a specific prostaglandin E (EP)2/4 receptor agonist, but not by sulprostone, a specific EP1/3 receptor agonist, indicating that cyclic AMP-coupled EP2/4 receptors are involved in the LBK-stimulated anion secretion. 6. A reverse transcriptase-polymerase chain reaction study detected the expression of COX-1 and COX-2 mRNA in intact rat epididymis and in cultured epididymal epithelia. The expression of COX-1 mRNA was reduced by LBK by 44%. 7. Immunohistochemical studies demonstrated the presence of COX-1 immunoreactivity in the basal cells of the intact rat epididymis. By comparision, COX-2 immunoreactivity was detected in the apical pole of the principal cells. 8. The role of COX in the formation of the epididymal microenvironment and the implication of long term administration of non-steroidal anti-inflammatory drugs (NSAIDs) on male fertility are discussed.
Persistent Identifierhttp://hdl.handle.net/10722/49360
ISSN
2015 Impact Factor: 4.731
2015 SCImago Journal Rankings: 2.670
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWong, PYDen_HK
dc.contributor.authorChan, HCen_HK
dc.contributor.authorLeung, PSen_HK
dc.contributor.authorChung, YWen_HK
dc.contributor.authorWong, YLen_HK
dc.contributor.authorLee, WMen_HK
dc.contributor.authorNg, Ven_HK
dc.contributor.authorDun, NJen_HK
dc.date.accessioned2008-06-12T06:40:20Z-
dc.date.available2008-06-12T06:40:20Z-
dc.date.issued1999en_HK
dc.identifier.citationJournal Of Physiology, 1999, v. 514 n. 3, p. 809-820en_HK
dc.identifier.issn0022-3751en_HK
dc.identifier.urihttp://hdl.handle.net/10722/49360-
dc.description.abstract1. The role of cyclo-oxygenase (COX) in the regulation of anion secretion (measured as short-circuit current, I(SC)) in cultured epididymal epithelia from immature rats was investigated. 2. COX inhibitors attenuated the increase of anion secretion caused by bradykinin (LBK) but had no effect on that caused by PGE 2, suggesting that prostaglandin synthesis mediates the secretory response of the tissues to LBK. 3. The apparent IC 50 values for indomethacin, piroxicam and L-745,337 in inhibiting the LBK-induced I(SC) were 0.14, 1.34 and 15.7 μM, respectively. This order of potency: indomethacin > piroxicam > L-745,337 >> DFU suggests the involvement of the COX-1 isozyme in the mediation of the secretory response to LBK. 4. Among the COX products (prostaglandins, thromboxane and prostacyclins) tested, only PGE 2 and, to a much lesser extent, PGF(2α) stimulated anion secretion by cultured rat epididymal epithelia. 5. The effect of PGE 2 was mimicked by 11-deoxyl PGE 1, a specific prostaglandin E (EP)2/4 receptor agonist, but not by sulprostone, a specific EP1/3 receptor agonist, indicating that cyclic AMP-coupled EP2/4 receptors are involved in the LBK-stimulated anion secretion. 6. A reverse transcriptase-polymerase chain reaction study detected the expression of COX-1 and COX-2 mRNA in intact rat epididymis and in cultured epididymal epithelia. The expression of COX-1 mRNA was reduced by LBK by 44%. 7. Immunohistochemical studies demonstrated the presence of COX-1 immunoreactivity in the basal cells of the intact rat epididymis. By comparision, COX-2 immunoreactivity was detected in the apical pole of the principal cells. 8. The role of COX in the formation of the epididymal microenvironment and the implication of long term administration of non-steroidal anti-inflammatory drugs (NSAIDs) on male fertility are discussed.en_HK
dc.format.extent388 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherWiley-Blackwell Publishing Ltd.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0022-3751en_HK
dc.relation.ispartofJournal of Physiologyen_HK
dc.rightsThe Journal of Physiology. Copyright © Blackwell Publishing Ltd.en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsThe definitive version is available at www.blackwell-synergy.comen_HK
dc.subject.meshEpididymis - cytology - enzymology - metabolismen_HK
dc.subject.meshProstaglandin-Endoperoxide Synthases - biosynthesis - metabolismen_HK
dc.subject.meshProstaglandins - pharmacologyen_HK
dc.subject.meshRNA, Messenger - biosynthesisen_HK
dc.subject.meshRats, Sprague-Dawleyen_HK
dc.titleRegulation of anion secretion by cyclo-oxygenase and prostanoids in cultured epididymal epithelia from the raten_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-3751&volume=514&issue=3&spage=809&epage=820&date=1999&atitle=Regulation+of+anion+secretion+by+cyclo-oxygenase+and+prostanoids+in+cultured+epididymal+epithelia+from+the+raten_HK
dc.identifier.emailLee, WM: hrszlwm@hku.hken_HK
dc.identifier.authorityLee, WM=rp00728en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1111/j.1469-7793.1999.809ad.xen_HK
dc.identifier.pmid9882752-
dc.identifier.pmcidPMC2269101en_HK
dc.identifier.scopuseid_2-s2.0-0033082103en_HK
dc.identifier.hkuros42368-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033082103&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume514en_HK
dc.identifier.issue3en_HK
dc.identifier.spage809en_HK
dc.identifier.epage820en_HK
dc.identifier.isiWOS:000079018600018-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridWong, PYD=7403980262en_HK
dc.identifier.scopusauthoridChan, HC=7403402737en_HK
dc.identifier.scopusauthoridLeung, PS=7401748938en_HK
dc.identifier.scopusauthoridChung, YW=7404388001en_HK
dc.identifier.scopusauthoridWong, YL=36853077800en_HK
dc.identifier.scopusauthoridLee, WM=24799156600en_HK
dc.identifier.scopusauthoridNg, V=7102162937en_HK
dc.identifier.scopusauthoridDun, NJ=7004670725en_HK

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