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Article: Adaptation of very virulent infectious bursal disease virus to chicken embryonic fibroblasts by site-directed mutagenesis of residues 279 and 284 of viral coat protein VP2

TitleAdaptation of very virulent infectious bursal disease virus to chicken embryonic fibroblasts by site-directed mutagenesis of residues 279 and 284 of viral coat protein VP2
Authors
Issue Date1999
PublisherAmerican Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/
Citation
Journal Of Virology, 1999, v. 73 n. 4, p. 2854-2862 How to Cite?
AbstractThe full-length RNA genomes of a chicken embryonic fibroblast (CEF)- nonpermissive, very virulent infectious bursal disease virus (IBDV) (strain HK46) were amplified into cDNAs by reverse transcription-PCR. The full- length cDNAs were sequenced and subcloned into a eukaryotic expression vector, from which point mutations were introduced into the VP2 region by site-directed mutagenesis. The wild-type and mutated plasmids were transfected directly into CEFs to examine their ability to generate CEF- permissive recombinant viruses. Substitution of amino acid residues 279 (Asp→Asn) and 284 (Ala→Thr) of the VP2 protein yielded a recombinant virus which was able to be passaged in CEFs, whereas the wild-type cDNAs and an amino acid substitution at residue 330 (Ser→Arg) of the VP2 protein alone did not yield viable virus. The results indicated that mutation of other vital proteins, including VP1, VP3, VP4, and VP5, was not required for CEF adaptation of the virus. The same approach may be used to produce CEF- adapted strains from newly evolved IBDVs or to manipulate the antigenicity of the virus.
Persistent Identifierhttp://hdl.handle.net/10722/49357
ISSN
2015 Impact Factor: 4.606
2015 SCImago Journal Rankings: 3.347
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLim, BLen_HK
dc.contributor.authorCao, Yen_HK
dc.contributor.authorYu, Ten_HK
dc.contributor.authorMo, CWen_HK
dc.date.accessioned2008-06-12T06:40:16Z-
dc.date.available2008-06-12T06:40:16Z-
dc.date.issued1999en_HK
dc.identifier.citationJournal Of Virology, 1999, v. 73 n. 4, p. 2854-2862en_HK
dc.identifier.issn0022-538Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/49357-
dc.description.abstractThe full-length RNA genomes of a chicken embryonic fibroblast (CEF)- nonpermissive, very virulent infectious bursal disease virus (IBDV) (strain HK46) were amplified into cDNAs by reverse transcription-PCR. The full- length cDNAs were sequenced and subcloned into a eukaryotic expression vector, from which point mutations were introduced into the VP2 region by site-directed mutagenesis. The wild-type and mutated plasmids were transfected directly into CEFs to examine their ability to generate CEF- permissive recombinant viruses. Substitution of amino acid residues 279 (Asp→Asn) and 284 (Ala→Thr) of the VP2 protein yielded a recombinant virus which was able to be passaged in CEFs, whereas the wild-type cDNAs and an amino acid substitution at residue 330 (Ser→Arg) of the VP2 protein alone did not yield viable virus. The results indicated that mutation of other vital proteins, including VP1, VP3, VP4, and VP5, was not required for CEF adaptation of the virus. The same approach may be used to produce CEF- adapted strains from newly evolved IBDVs or to manipulate the antigenicity of the virus.en_HK
dc.format.extent420 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherAmerican Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/en_HK
dc.relation.ispartofJournal of Virologyen_HK
dc.rightsJournal of Virology. Copyright © American Society for Microbiology.en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsCopyright © American Society for Microbiology, Journal of Virology, 1999, v. 73 n. 4, p. 2854-2862en_HK
dc.subject.meshBirnaviridae Infections - genetics - virologyen_HK
dc.subject.meshFibroblasts - virologyen_HK
dc.subject.meshInfectious bursal disease virus - geneticsen_HK
dc.subject.meshViral Structural Proteins - geneticsen_HK
dc.subject.meshAmino Acid Sequenceen_HK
dc.titleAdaptation of very virulent infectious bursal disease virus to chicken embryonic fibroblasts by site-directed mutagenesis of residues 279 and 284 of viral coat protein VP2en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-538X&volume=73&issue=4&spage=2854&epage=2862&date=1999&atitle=Adaptation+of+very+virulent+infectious+bursal+disease+virus+to+chicken+embryonic+fibroblasts+by+site-directed+mutagenesis+of+residues+279+and+284+of+viral+coat+protein+VP2en_HK
dc.identifier.emailLim, BL: bllim@hkucc.hku.hken_HK
dc.identifier.authorityLim, BL=rp00744en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.pmid10074133-
dc.identifier.pmcidPMC104043-
dc.identifier.scopuseid_2-s2.0-0033044128en_HK
dc.identifier.hkuros40376-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033044128&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume73en_HK
dc.identifier.issue4en_HK
dc.identifier.spage2854en_HK
dc.identifier.epage2862en_HK
dc.identifier.isiWOS:000079122400033-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLim, BL=7201983917en_HK
dc.identifier.scopusauthoridCao, Y=25632098400en_HK
dc.identifier.scopusauthoridYu, T=7401861669en_HK
dc.identifier.scopusauthoridMo, CW=7005256318en_HK

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