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Article: Rat testicular N-cadherin: Its complementary deoxyribonucleic acid cloning and regulation

TitleRat testicular N-cadherin: Its complementary deoxyribonucleic acid cloning and regulation
Authors
Issue Date1998
PublisherThe Endocrine Society. The Journal's web site is located at http://endo.endojournals.org
Citation
Endocrinology, 1998, v. 139 n. 4, p. 1853-1862 How to Cite?
AbstractUsing primer sets specific for mouse N-cadherin and rat testicular RNA for RT-PCR, a full-length complementary DNA (cDNA) coding for rat testicular N-cadherin was isolated. The deduced amino acid sequence of rat N-cadherin yielded a 883-amino acid polypeptide that displayed a 98.6% identity with the mouse homolog. N-Cadherin was found to be expressed by Sertoli and germ cells in the rat testis by RT-PCR. Using Sertoli-germ cell cocultures, it was found that the N-cadherin expression increased with time in culture. To assess whether this is due to a soluble factor(s) released from germ cells that affects Sertoli cell N-cadherin expression, germ cell-conditioned media (GCCM) were fractionated by preparative anion-exchange HPLC, and the resulting fractions were divided into 14 pools. Pool 4 was found to contain a factor(s) that induced a dose-dependent stimulation on Sertoli cell N- cadherin expression with a maximal stimulation at 2 μg protein/dish/4.5 x 106 Sertoli cells. At higher doses between 12 and 32 μg protein/dish, this pool relinquished its effect on Sertoli cell N-cadherin expression suggestive of a biphasic effect. This biphasic effect was confirmed using increasing doses of crude GCCM on Sertoli cell cultures. Since nonviable germ cells failed to stimulate Sertoli cell N-cadherin expression, it illustrates the observed stimulatory effect by GCCM is likely to be mediated via a soluble factor(s) releasing from viable germ cells. These results reveal the presence of a stimulatory factor(s) in GCCM that can modulate Sertoli cell N-cadherin expression in vitro. Since N-cadherin plays a crucial role in facilitating invasive capacity of metastatic tumor cells, the observation of germ cell- released factor(s) in affecting Sertoli cell N-cadherin expression may suggest its possible role in facilitating germ cell migration during spermatogenesis.
Persistent Identifierhttp://hdl.handle.net/10722/49353
ISSN
2015 Impact Factor: 4.159
2015 SCImago Journal Rankings: 2.363
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChung, SSWen_HK
dc.contributor.authorMo, MYen_HK
dc.contributor.authorSilvestrini, Ben_HK
dc.contributor.authorLee, WMen_HK
dc.contributor.authorCheng, CYen_HK
dc.date.accessioned2008-06-12T06:40:10Z-
dc.date.available2008-06-12T06:40:10Z-
dc.date.issued1998en_HK
dc.identifier.citationEndocrinology, 1998, v. 139 n. 4, p. 1853-1862en_HK
dc.identifier.issn0013-7227en_HK
dc.identifier.urihttp://hdl.handle.net/10722/49353-
dc.description.abstractUsing primer sets specific for mouse N-cadherin and rat testicular RNA for RT-PCR, a full-length complementary DNA (cDNA) coding for rat testicular N-cadherin was isolated. The deduced amino acid sequence of rat N-cadherin yielded a 883-amino acid polypeptide that displayed a 98.6% identity with the mouse homolog. N-Cadherin was found to be expressed by Sertoli and germ cells in the rat testis by RT-PCR. Using Sertoli-germ cell cocultures, it was found that the N-cadherin expression increased with time in culture. To assess whether this is due to a soluble factor(s) released from germ cells that affects Sertoli cell N-cadherin expression, germ cell-conditioned media (GCCM) were fractionated by preparative anion-exchange HPLC, and the resulting fractions were divided into 14 pools. Pool 4 was found to contain a factor(s) that induced a dose-dependent stimulation on Sertoli cell N- cadherin expression with a maximal stimulation at 2 μg protein/dish/4.5 x 106 Sertoli cells. At higher doses between 12 and 32 μg protein/dish, this pool relinquished its effect on Sertoli cell N-cadherin expression suggestive of a biphasic effect. This biphasic effect was confirmed using increasing doses of crude GCCM on Sertoli cell cultures. Since nonviable germ cells failed to stimulate Sertoli cell N-cadherin expression, it illustrates the observed stimulatory effect by GCCM is likely to be mediated via a soluble factor(s) releasing from viable germ cells. These results reveal the presence of a stimulatory factor(s) in GCCM that can modulate Sertoli cell N-cadherin expression in vitro. Since N-cadherin plays a crucial role in facilitating invasive capacity of metastatic tumor cells, the observation of germ cell- released factor(s) in affecting Sertoli cell N-cadherin expression may suggest its possible role in facilitating germ cell migration during spermatogenesis.en_HK
dc.format.extent418 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherThe Endocrine Society. The Journal's web site is located at http://endo.endojournals.orgen_HK
dc.relation.ispartofEndocrinologyen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsEndocrinology. Copyright © The Endocrine Society.en_HK
dc.subject.meshCadherins - chemistry - geneticsen_HK
dc.subject.meshCloning, Molecularen_HK
dc.subject.meshDNA, Complementary - chemistry - geneticsen_HK
dc.subject.meshTestis - chemistryen_HK
dc.subject.meshAmino Acid Sequenceen_HK
dc.titleRat testicular N-cadherin: Its complementary deoxyribonucleic acid cloning and regulationen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0013-7227&volume=139&issue=4&spage=1853&epage=1862&date=1998&atitle=Rat+testicular+N-cadherin:+its+complementary+deoxyribonucleic+acid+cloning+and+regulationen_HK
dc.identifier.emailLee, WM: hrszlwm@hku.hken_HK
dc.identifier.authorityLee, WM=rp00728en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.pmid9528971-
dc.identifier.scopuseid_2-s2.0-0031732607en_HK
dc.identifier.hkuros31185-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031732607&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume139en_HK
dc.identifier.issue4en_HK
dc.identifier.spage1853en_HK
dc.identifier.epage1862en_HK
dc.identifier.isiWOS:000072669600050-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChung, SSW=35104555300en_HK
dc.identifier.scopusauthoridMo, MY=7006857340en_HK
dc.identifier.scopusauthoridSilvestrini, B=7006825900en_HK
dc.identifier.scopusauthoridLee, WM=24799156600en_HK
dc.identifier.scopusauthoridCheng, CY=7404797787en_HK

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