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Article: Steroidogenic factor 1 and estradiol receptor act in synergism to regulate the expression of the salmon gonadotropin IIβ subunit gene

TitleSteroidogenic factor 1 and estradiol receptor act in synergism to regulate the expression of the salmon gonadotropin IIβ subunit gene
Authors
Issue Date1996
PublisherEndocrine Society. The Journal's web site is located at http://mend.endojournals.org/
Citation
Molecular Endocrinology, 1996, v. 10 n. 3, p. 217-229 How to Cite?
AbstractThe orphan nuclear receptor steroidogenic factor-1 (SF-1) regulates the expression of several genes involved in the reproductive function and development of the adrenal, the gonads, and the pituitary gonadotropes. It also confers the gonadotrope-specific expression of the glycoprotein hormone α subunit gene by the binding to a gonadotrope-specific element (GSE). In this study, we have shown that SF-1 transactivates the salmon gonadotropin IIβ subunit (sGTHIIβ) gene expression. SF-1 alone offered a slight but significant enhancement on sGTHIIβ promoter activity (7.2 ± 0.6 fold). However, it stimulated sGTHIIβ gene expression dramatically (127 ± 37 fold) when combined with the estrogen receptor (ER). This synergistic interaction was specific for sGTHIIβ promoter as well as for both SF-1 and ER and was estradiol-dose dependent. 5'-Deletion studies of the sGTHIIβ promoter identified two putative SF-1 binding sites (GSE) and one previously identified proximal estrogen-responsive element (pERE) at -274 bp involved in this activation. The two GSE sequences located at -354 bp (sGSE 3) and -162 bp (sGSE 2) upstream of the transcription site, although imperfect as compared with the consensus GSE, bound specifically to the in vitro- translated mouse SF-1 protein. 5'-Deletion studies, competition experiments, and site-directed mutagenesis showed that binding to pERE and GSE 2 were necessary for the SF-1/ER synergistic effect. These studies suggest that the synergistic interaction of SF-1 and ER, possibly through cooperative binding or protein-protein interaction, is essential in conferring a cell type- specific expression of the GTHIIβ subunit gene.
Persistent Identifierhttp://hdl.handle.net/10722/49351
ISSN
2015 Impact Factor: 3.432
2015 SCImago Journal Rankings: 2.195
References

 

DC FieldValueLanguage
dc.contributor.authorLe Dréan, Yen_HK
dc.contributor.authorLiu, Den_HK
dc.contributor.authorWong, AOLen_HK
dc.contributor.authorXiong, Fen_HK
dc.contributor.authorHew, CLen_HK
dc.date.accessioned2008-06-12T06:40:07Z-
dc.date.available2008-06-12T06:40:07Z-
dc.date.issued1996en_HK
dc.identifier.citationMolecular Endocrinology, 1996, v. 10 n. 3, p. 217-229en_HK
dc.identifier.issn0888-8809en_HK
dc.identifier.urihttp://hdl.handle.net/10722/49351-
dc.description.abstractThe orphan nuclear receptor steroidogenic factor-1 (SF-1) regulates the expression of several genes involved in the reproductive function and development of the adrenal, the gonads, and the pituitary gonadotropes. It also confers the gonadotrope-specific expression of the glycoprotein hormone α subunit gene by the binding to a gonadotrope-specific element (GSE). In this study, we have shown that SF-1 transactivates the salmon gonadotropin IIβ subunit (sGTHIIβ) gene expression. SF-1 alone offered a slight but significant enhancement on sGTHIIβ promoter activity (7.2 ± 0.6 fold). However, it stimulated sGTHIIβ gene expression dramatically (127 ± 37 fold) when combined with the estrogen receptor (ER). This synergistic interaction was specific for sGTHIIβ promoter as well as for both SF-1 and ER and was estradiol-dose dependent. 5'-Deletion studies of the sGTHIIβ promoter identified two putative SF-1 binding sites (GSE) and one previously identified proximal estrogen-responsive element (pERE) at -274 bp involved in this activation. The two GSE sequences located at -354 bp (sGSE 3) and -162 bp (sGSE 2) upstream of the transcription site, although imperfect as compared with the consensus GSE, bound specifically to the in vitro- translated mouse SF-1 protein. 5'-Deletion studies, competition experiments, and site-directed mutagenesis showed that binding to pERE and GSE 2 were necessary for the SF-1/ER synergistic effect. These studies suggest that the synergistic interaction of SF-1 and ER, possibly through cooperative binding or protein-protein interaction, is essential in conferring a cell type- specific expression of the GTHIIβ subunit gene.en_HK
dc.format.extent418 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherEndocrine Society. The Journal's web site is located at http://mend.endojournals.org/en_HK
dc.relation.ispartofMolecular Endocrinologyen_HK
dc.rightsMolecular Endocrinology. Copyright © The Endocrine Society.en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshDNA-Binding Proteins - physiologyen_HK
dc.subject.meshGene Expression Regulationen_HK
dc.subject.meshGonadotropins, Pituitary - biosynthesis - geneticsen_HK
dc.subject.meshReceptors, Estradiol - physiologyen_HK
dc.subject.meshSalmon - geneticsen_HK
dc.titleSteroidogenic factor 1 and estradiol receptor act in synergism to regulate the expression of the salmon gonadotropin IIβ subunit geneen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0888-8809&volume=10&issue=3&spage=217&epage=229&date=1996&atitle=Steroidogenic+factor+1+and+estradiol+receptor+act+in+synergism+to+regulate+the+expression+of+the+salmon+gonadotropin+IIβ+subunit+geneen_HK
dc.identifier.emailWong, AOL: olwong@hkucc.hku.hken_HK
dc.identifier.authorityWong, AOL=rp00806en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1210/me.10.3.217en_HK
dc.identifier.pmid8833651-
dc.identifier.scopuseid_2-s2.0-0029882082en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0029882082&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume10en_HK
dc.identifier.issue3en_HK
dc.identifier.spage217en_HK
dc.identifier.epage229en_HK
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLe Dréan, Y=6603392595en_HK
dc.identifier.scopusauthoridLiu, D=36706226300en_HK
dc.identifier.scopusauthoridWong, AOL=7403147570en_HK
dc.identifier.scopusauthoridXiong, F=7102471913en_HK
dc.identifier.scopusauthoridHew, CL=7007113098en_HK

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