File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: CTL control of EBV in nasopharyngeal carcinoma (NPC): EBV-specific CTL responses in the blood and tumors of NPC patients and the antigen-processing function of the tumor cells

TitleCTL control of EBV in nasopharyngeal carcinoma (NPC): EBV-specific CTL responses in the blood and tumors of NPC patients and the antigen-processing function of the tumor cells
Authors
Issue Date2000
PublisherAmerican Association of Immunologists. The Journal's web site is located at http://www.jimmunol.org
Citation
Journal Of Immunology, 2000, v. 165 n. 1, p. 573-582 How to Cite?
AbstractUndifferentiated nasopharyngeal carcinoma (NPC) is latently infected with EBV and expresses a restricted number of viral proteins. Studies in healthy virus carriers have demonstrated that at least some of these proteins can act as targets for HLA class I-restricted CTLs. Therefore we have explored the possibility of a CTL-based therapy for NPC by characterizing EBV-specific CTL responses in 10 newly diagnosed NPC cases and 21 healthy virus carriers from Southeast Asia. Using the autologous EBV-transformed lymphoblastoid cell line, virus-specific CTL were reactivated in vitro from PBMC, cloned, and screened for cytotoxicity against target cells expressing individual EBV proteins from recombinant vaccinia vectors. EBV-specific CTLs were identified in 6 of 10 patients and 14 of 21 controls and mainly targeted the EBV nuclear Ag 3 (EBNA3) family of viral latent proteins. However, in 3 of 10 patients and 11 of 21 controls, CTLs specific for the NPC-associated protein LMP2 were also detected, albeit at low frequency. EBV-specific CTLs were detected in tumor biopsy material obtained from 3 of 6 of the patients, indicating that functional CTL are present at the tumor site, but none was specific for tumor-associated viral proteins. To assess the Ag-presenting function in NPC we studied two NPC-derived cell lines (C15 and c666.1) and demonstrated that both were capable of processing and presenting endogenously synthesized protein to HLA class I-restricted CTL clones. Overall, our data provide a sound theoretical basis for therapeutic strategies that aim to boost or elicit LMP2-specific CTL responses in NPC patients.
Persistent Identifierhttp://hdl.handle.net/10722/49335
ISSN
2015 Impact Factor: 4.985
2015 SCImago Journal Rankings: 3.549
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLee, SPen_HK
dc.contributor.authorChan, ATCen_HK
dc.contributor.authorCheung, STen_HK
dc.contributor.authorThomas, WAen_HK
dc.contributor.authorCroomCarter, Den_HK
dc.contributor.authorDawson, CWen_HK
dc.contributor.authorTsai, CHen_HK
dc.contributor.authorLeung, SFen_HK
dc.contributor.authorJohnson, PJen_HK
dc.contributor.authorHuang, DPen_HK
dc.date.accessioned2008-06-12T06:39:45Z-
dc.date.available2008-06-12T06:39:45Z-
dc.date.issued2000en_HK
dc.identifier.citationJournal Of Immunology, 2000, v. 165 n. 1, p. 573-582en_HK
dc.identifier.issn0022-1767en_HK
dc.identifier.urihttp://hdl.handle.net/10722/49335-
dc.description.abstractUndifferentiated nasopharyngeal carcinoma (NPC) is latently infected with EBV and expresses a restricted number of viral proteins. Studies in healthy virus carriers have demonstrated that at least some of these proteins can act as targets for HLA class I-restricted CTLs. Therefore we have explored the possibility of a CTL-based therapy for NPC by characterizing EBV-specific CTL responses in 10 newly diagnosed NPC cases and 21 healthy virus carriers from Southeast Asia. Using the autologous EBV-transformed lymphoblastoid cell line, virus-specific CTL were reactivated in vitro from PBMC, cloned, and screened for cytotoxicity against target cells expressing individual EBV proteins from recombinant vaccinia vectors. EBV-specific CTLs were identified in 6 of 10 patients and 14 of 21 controls and mainly targeted the EBV nuclear Ag 3 (EBNA3) family of viral latent proteins. However, in 3 of 10 patients and 11 of 21 controls, CTLs specific for the NPC-associated protein LMP2 were also detected, albeit at low frequency. EBV-specific CTLs were detected in tumor biopsy material obtained from 3 of 6 of the patients, indicating that functional CTL are present at the tumor site, but none was specific for tumor-associated viral proteins. To assess the Ag-presenting function in NPC we studied two NPC-derived cell lines (C15 and c666.1) and demonstrated that both were capable of processing and presenting endogenously synthesized protein to HLA class I-restricted CTL clones. Overall, our data provide a sound theoretical basis for therapeutic strategies that aim to boost or elicit LMP2-specific CTL responses in NPC patients.en_HK
dc.format.extent420 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherAmerican Association of Immunologists. The Journal's web site is located at http://www.jimmunol.orgen_HK
dc.relation.ispartofJournal of Immunologyen_HK
dc.rightsThis is an author-produced version of a manuscript accepted for publication in The Journal of Immunology (The JI). The American Association of Immunologists, Inc. (The AAI), publisher of The JI, holds the copyright to this manuscript. This manuscript has not yet been copyedited or subjected to editorial proofreading by The JI; hence, it may differ from the final version published in The JI (online and in print). The AAI (The JI) is not liable for errors or omissions in this author-produced version of the manuscript or in any version derived from it by the National Institutes of Health or any other third party. The final, citable version of record can be found at www.jimmunol.orgen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshAntigen-Presenting Cells - immunology - metabolism - pathologyen_HK
dc.subject.meshCytotoxicity, Immunologicen_HK
dc.subject.meshHerpesvirus 4, Human - immunologyen_HK
dc.subject.meshNasopharyngeal Neoplasms - blood - immunology - pathology - therapyen_HK
dc.subject.meshT-Lymphocytes, Cytotoxic - immunology - virologyen_HK
dc.titleCTL control of EBV in nasopharyngeal carcinoma (NPC): EBV-specific CTL responses in the blood and tumors of NPC patients and the antigen-processing function of the tumor cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-1767&volume=165&issue=1&spage=573&epage=582&date=2000&atitle=CTL+control+of+EBV+in+nasopharyngeal+carcinoma+(NPC):+EBV-specific+CTL+resopnses+in+the+blood+and+tumors+of+NPC+patients+and+the+antigen-processing+function+of+the+tumor+cellsen_HK
dc.identifier.emailCheung, ST: stcheung@hkucc.hku.hken_HK
dc.identifier.authorityCheung, ST=rp00457en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.pmid10861098-
dc.identifier.scopuseid_2-s2.0-0034235814en_HK
dc.identifier.hkuros61180-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034235814&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume165en_HK
dc.identifier.issue1en_HK
dc.identifier.spage573en_HK
dc.identifier.epage582en_HK
dc.identifier.isiWOS:000087816800073-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLee, SP=8146042300en_HK
dc.identifier.scopusauthoridChan, ATC=13404833700en_HK
dc.identifier.scopusauthoridCheung, ST=7202473497en_HK
dc.identifier.scopusauthoridThomas, WA=35557128200en_HK
dc.identifier.scopusauthoridCroomCarter, D=6505718817en_HK
dc.identifier.scopusauthoridDawson, CW=7202226918en_HK
dc.identifier.scopusauthoridTsai, CH=24391420500en_HK
dc.identifier.scopusauthoridLeung, SF=7202044876en_HK
dc.identifier.scopusauthoridJohnson, PJ=7405661637en_HK
dc.identifier.scopusauthoridHuang, DP=7403891486en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats