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Article: Nonphlogistic clearance of late apoptotic neutrophils by macrophages: efficient phagocytosis independent of β2 intergrins

TitleNonphlogistic clearance of late apoptotic neutrophils by macrophages: efficient phagocytosis independent of β2 intergrins
Authors
Issue Date2001
PublisherAmerican Association of Immunologists. The Journal's web site is located at http://www.jimmunol.org
Citation
Journal of Immunology, 2001, v. 166 n. 7, p. 4743-4750 How to Cite?
AbstractNeutrophils undergo constitutive death by apoptosis, leading to safe nonphlogistic phagocytosis and clearance by macrophages. Recent work has shown that before secondary necrosis, neutrophils exhibiting classical features of apoptosis can progress to a morphologically defined late apoptotic state. However, whether such neutrophils could be safely cleared was unknown. We now report that human late apoptotic neutrophils could be purified from cultured neutrophil populations undergoing constitutive death and were subsequently ingested by human monocyte-derived macrophages by serum-independent mechanisms that did not trigger the release of IL-8 or TNF-alpha. Such ingestion was specifically inhibited by Abs to thrombospondin-1 and the alpha(v)beta(3) vitronectin receptor. Murine bone marrow-derived macrophage phagocytosis of late and early apoptotic neutrophils occurred by similar mechanisms, proceeding with the same efficiency as that observed for wild-type controls when macrophages from [alpha(m)](-/-) or [beta(2)](-/-) mice were used. We conclude that specific nonphlogistic, beta(2) integrin-independent mechanisms involving thrombospondin-1 and alpha(v)beta(3) allow macrophages to ingest late apoptotic neutrophils without eliciting inflammatory cytokine secretion.
Persistent Identifierhttp://hdl.handle.net/10722/49330
ISSN
2015 Impact Factor: 4.985
2015 SCImago Journal Rankings: 3.549
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorRen, Yen_HK
dc.contributor.authorStuart, Len_HK
dc.contributor.authorLindberg, FPen_HK
dc.contributor.authorRosenkranz, ARen_HK
dc.contributor.authorChen, Yen_HK
dc.contributor.authorMayadas, TNen_HK
dc.contributor.authorSavill, Jen_HK
dc.date.accessioned2008-06-12T06:39:38Z-
dc.date.available2008-06-12T06:39:38Z-
dc.date.issued2001en_HK
dc.identifier.citationJournal of Immunology, 2001, v. 166 n. 7, p. 4743-4750en_HK
dc.identifier.issn0022-1767en_HK
dc.identifier.urihttp://hdl.handle.net/10722/49330-
dc.description.abstractNeutrophils undergo constitutive death by apoptosis, leading to safe nonphlogistic phagocytosis and clearance by macrophages. Recent work has shown that before secondary necrosis, neutrophils exhibiting classical features of apoptosis can progress to a morphologically defined late apoptotic state. However, whether such neutrophils could be safely cleared was unknown. We now report that human late apoptotic neutrophils could be purified from cultured neutrophil populations undergoing constitutive death and were subsequently ingested by human monocyte-derived macrophages by serum-independent mechanisms that did not trigger the release of IL-8 or TNF-alpha. Such ingestion was specifically inhibited by Abs to thrombospondin-1 and the alpha(v)beta(3) vitronectin receptor. Murine bone marrow-derived macrophage phagocytosis of late and early apoptotic neutrophils occurred by similar mechanisms, proceeding with the same efficiency as that observed for wild-type controls when macrophages from [alpha(m)](-/-) or [beta(2)](-/-) mice were used. We conclude that specific nonphlogistic, beta(2) integrin-independent mechanisms involving thrombospondin-1 and alpha(v)beta(3) allow macrophages to ingest late apoptotic neutrophils without eliciting inflammatory cytokine secretion.en_HK
dc.format.extent420 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherAmerican Association of Immunologists. The Journal's web site is located at http://www.jimmunol.orgen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsThis is an author-produced version of a manuscript accepted for publication in The Journal of Immunology (The JI). The American Association of Immunologists, Inc. (The AAI), publisher of The JI, holds the copyright to this manuscript. This manuscript has not yet been copyedited or subjected to editorial proofreading by The JI; hence, it may differ from the final version published in The JI (online and in print). The AAI (The JI) is not liable for errors or omissions in this author-produced version of the manuscript or in any version derived from it by the National Institutes of Health or any other third party. The final, citable version of record can be found at www.jimmunol.orgen_HK
dc.subject.meshApoptosis - genetics - immunologyen_HK
dc.subject.meshIntegrins - physiologyen_HK
dc.subject.meshMacrophages - immunology - metabolismen_HK
dc.subject.meshNeutrophils - cytology - immunology - metabolismen_HK
dc.subject.meshPhagocytosis - genetics - immunologyen_HK
dc.titleNonphlogistic clearance of late apoptotic neutrophils by macrophages: efficient phagocytosis independent of β2 intergrinsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-1767&volume=166&issue=7&spage=4743&epage=4750&date=2001&atitle=Nonphlogistic+clearance+of+late+apoptotic+neutrophils+by+macrophages:+efficient+phagocytosis+independent+of+β2+intergrinsen_HK
dc.identifier.emailRen, Y: yren@hkucc.hku.hken_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.pmid11254736en_HK
dc.identifier.isiWOS:000170948200059-

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