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Article: Small molecules blocking the entry of severe acute respiratory syndrome coronavirus into host cells

TitleSmall molecules blocking the entry of severe acute respiratory syndrome coronavirus into host cells
Authors
Issue Date2004
PublisherAmerican Society for Microbiology.
Citation
Journal of Virology, 2004, v. 78 n. 20, p. 11334-11339 How to Cite?
AbstractSevere acute respiratory syndrome coronavirus (SARS-CoV) is the pathogen of SARS, which caused a global panic in 2003. We describe here the screening of Chinese herbal medicine-based, novel small molecules that bind avidly with the surface spike protein of SARS-CoV and thus can interfere with the entry of the virus to its host cells. We achieved this by using a two-step screening method consisting of frontal affinity chromatography-mass spectrometry coupled with a viral infection assay based on a human immunodeficiency virus (HIV)-luc/SARS pseudotyped virus. Two small molecules, tetra-O-galloyl-beta-D-glucose (TGG) and luteolin, were identified, whose anti-SARS-CoV activities were confirmed by using a wild-type SARS-CoV infection system. TGG exhibits prominent anti-SARS-CoV activity with a 50% effective concentration of 4.5 microM and a selective index of 240.0. The two-step screening method described here yielded several small molecules that can be used for developing new classes of anti-SARS-CoV drugs and is potentially useful for the high-throughput screening of drugs inhibiting the entry of HIV, hepatitis C virus, and other insidious viruses into their host cells.
Persistent Identifierhttp://hdl.handle.net/10722/49313
ISSN
2015 Impact Factor: 4.606
2015 SCImago Journal Rankings: 3.347
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorYi, Len_HK
dc.contributor.authorLi, Zen_HK
dc.contributor.authorYuan, Ken_HK
dc.contributor.authorQu, Xen_HK
dc.contributor.authorChen, Jen_HK
dc.contributor.authorWang, Gen_HK
dc.contributor.authorZhang, Hen_HK
dc.contributor.authorLuo, Hen_HK
dc.contributor.authorZhu, Len_HK
dc.contributor.authorJiang, Pen_HK
dc.contributor.authorChen, Len_HK
dc.contributor.authorShen, Yen_HK
dc.contributor.authorLuo, Men_HK
dc.contributor.authorZuo, Gen_HK
dc.contributor.authorHu, Jen_HK
dc.contributor.authorDuan, Den_HK
dc.contributor.authorNie, Yen_HK
dc.contributor.authorShi, Xen_HK
dc.contributor.authorWang, Wen_HK
dc.contributor.authorHan, Yen_HK
dc.contributor.authorLi, Ten_HK
dc.contributor.authorLiu, Yen_HK
dc.contributor.authorDing, Men_HK
dc.contributor.authorDeng, Hen_HK
dc.contributor.authorXu, Xen_HK
dc.date.accessioned2008-06-12T06:39:13Z-
dc.date.available2008-06-12T06:39:13Z-
dc.date.issued2004en_HK
dc.identifier.citationJournal of Virology, 2004, v. 78 n. 20, p. 11334-11339en_HK
dc.identifier.issn0022-538Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/49313-
dc.description.abstractSevere acute respiratory syndrome coronavirus (SARS-CoV) is the pathogen of SARS, which caused a global panic in 2003. We describe here the screening of Chinese herbal medicine-based, novel small molecules that bind avidly with the surface spike protein of SARS-CoV and thus can interfere with the entry of the virus to its host cells. We achieved this by using a two-step screening method consisting of frontal affinity chromatography-mass spectrometry coupled with a viral infection assay based on a human immunodeficiency virus (HIV)-luc/SARS pseudotyped virus. Two small molecules, tetra-O-galloyl-beta-D-glucose (TGG) and luteolin, were identified, whose anti-SARS-CoV activities were confirmed by using a wild-type SARS-CoV infection system. TGG exhibits prominent anti-SARS-CoV activity with a 50% effective concentration of 4.5 microM and a selective index of 240.0. The two-step screening method described here yielded several small molecules that can be used for developing new classes of anti-SARS-CoV drugs and is potentially useful for the high-throughput screening of drugs inhibiting the entry of HIV, hepatitis C virus, and other insidious viruses into their host cells.en_HK
dc.format.extent386 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherAmerican Society for Microbiology.en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsJournal of Virology. Copyright © American Society for Microbiology.en_HK
dc.rightsCopyright © American Society for Microbiology, Journal of Virology, 2004, v. 78 n. 20, p. 11334-11339en_HK
dc.subject.meshAntiviral Agents - chemistry - metabolism - pharmacologyen_HK
dc.subject.meshFlavonoids - pharmacologyen_HK
dc.subject.meshHydrolyzable Tannins - chemistry - pharmacologyen_HK
dc.subject.meshPlants, Medicinal - chemistry - metabolismen_HK
dc.subject.meshSARS Virus - drug effects - pathogenicityen_HK
dc.titleSmall molecules blocking the entry of severe acute respiratory syndrome coronavirus into host cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-538X&volume=78&issue=20&spage=11334&epage=11339&date=2004&atitle=Small+molecules+blocking+the+entry+of+severe+acute+respiratory+syndrome+coronavirus+into+host+cellsen_HK
dc.identifier.emailLiu, Y: ayliu@hkucc.hku.hken_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1128/JVI.78.20.11334-11339.2004en_HK
dc.identifier.pmid15452254-
dc.identifier.pmcidPMC521800en_HK
dc.identifier.scopuseid_2-s2.0-4644282214-
dc.identifier.hkuros104447-
dc.identifier.isiWOS:000224229000051-

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