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- Publisher Website: 10.1038/sj.bjp.0705475
- Scopus: eid_2-s2.0-0242660884
- PMID: 14534156
- WOS: WOS:000186240600020
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Article: Roles of KATP channels in delayed cardioprotection and intracellular Ca2+ in the rat heart as revealed by κ-opioid receptor stimulation with U50,488H
Title | Roles of KATP channels in delayed cardioprotection and intracellular Ca2+ in the rat heart as revealed by κ-opioid receptor stimulation with U50,488H |
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Authors | |
Keywords | Kappa-opioid receptor Preconditioning ATP-sensitive potassium channel Intracellular Ca2+ U50,488H |
Issue Date | 2003 |
Publisher | Nature Publishing Group. The Journal's web site is located at http://www.nature.com/bjp |
Citation | British Journal of Pharmacology, 2003, v. 140 n. 4, p. 750-758 How to Cite? |
Abstract | The effect of preconditioning with U50488 H (UP), a selective kappa-opioid receptor (kappa-OR) agonist, on infarct size and intracellular Ca2+ ([Ca2+]i) in the heart subjected to ischaemic insults were studied and evaluated. U50488 H administered intravenously reduced the infarct size 18-48 h after administration in isolated hearts subjected to regional ischaemia/reperfusion (I/R). The effect was dose dependent. A peak effect was reached at 10 mg x kg-1 U50488 H and at 24 h after administration. The effect of 10 mg x kg-1 U50488 H at 24 h after administration was abolished by nor-binaltorphimine (nor-BNI), a selective kappa-OR antagonist, indicating the effect was kappa-OR mediated. The infarct reducing effect of U50488 H was attenuated when a selective blocker of mitochondrial (5-hydroxydecanoic acid, 5-HD) or sarcolemmal (HRM-1098) ATP-sensitive potassium channel (KATP) was coadministered with U50488 H 24 h before ischaemia or when 5-HD was administered just before ischaemia. U50488 H also attenuated the elevation in [Ca2+]i and reduction in electrically induced [Ca2+]i transient in cardiomyocytes subjected to ischaemic insults. The effects were reversed by blockade of KATP channel, which abolished the protective effect of preconditioning with U50488 H. The results indicated that mitochondrial KATP channel serves as both a trigger and a mediator, while sarcolemmal KATP channel as a trigger only, of delayed cardioprotection of kappa-OR stimulation. The effects of these channels may result from prevention/attenuation of [Ca2+]i overload induced by ischaemic insults. |
Persistent Identifier | http://hdl.handle.net/10722/49309 |
ISSN | 2021 Impact Factor: 9.473 2020 SCImago Journal Rankings: 2.432 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Chen, M | en_HK |
dc.contributor.author | Zhou, JJ | en_HK |
dc.contributor.author | Kam, KWL | en_HK |
dc.contributor.author | Qi, JS | en_HK |
dc.contributor.author | Yan, WY | en_HK |
dc.contributor.author | Wu, S | en_HK |
dc.contributor.author | Wong, TM | en_HK |
dc.date.accessioned | 2008-06-12T06:39:02Z | - |
dc.date.available | 2008-06-12T06:39:02Z | - |
dc.date.issued | 2003 | en_HK |
dc.identifier.citation | British Journal of Pharmacology, 2003, v. 140 n. 4, p. 750-758 | en_HK |
dc.identifier.issn | 0007-1188 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/49309 | - |
dc.description.abstract | The effect of preconditioning with U50488 H (UP), a selective kappa-opioid receptor (kappa-OR) agonist, on infarct size and intracellular Ca2+ ([Ca2+]i) in the heart subjected to ischaemic insults were studied and evaluated. U50488 H administered intravenously reduced the infarct size 18-48 h after administration in isolated hearts subjected to regional ischaemia/reperfusion (I/R). The effect was dose dependent. A peak effect was reached at 10 mg x kg-1 U50488 H and at 24 h after administration. The effect of 10 mg x kg-1 U50488 H at 24 h after administration was abolished by nor-binaltorphimine (nor-BNI), a selective kappa-OR antagonist, indicating the effect was kappa-OR mediated. The infarct reducing effect of U50488 H was attenuated when a selective blocker of mitochondrial (5-hydroxydecanoic acid, 5-HD) or sarcolemmal (HRM-1098) ATP-sensitive potassium channel (KATP) was coadministered with U50488 H 24 h before ischaemia or when 5-HD was administered just before ischaemia. U50488 H also attenuated the elevation in [Ca2+]i and reduction in electrically induced [Ca2+]i transient in cardiomyocytes subjected to ischaemic insults. The effects were reversed by blockade of KATP channel, which abolished the protective effect of preconditioning with U50488 H. The results indicated that mitochondrial KATP channel serves as both a trigger and a mediator, while sarcolemmal KATP channel as a trigger only, of delayed cardioprotection of kappa-OR stimulation. The effects of these channels may result from prevention/attenuation of [Ca2+]i overload induced by ischaemic insults. | en_HK |
dc.format.extent | 388 bytes | - |
dc.format.mimetype | text/html | - |
dc.language | eng | en_HK |
dc.publisher | Nature Publishing Group. The Journal's web site is located at http://www.nature.com/bjp | en_HK |
dc.relation.ispartof | British Journal of Pharmacology | - |
dc.subject | Kappa-opioid receptor | en_HK |
dc.subject | Preconditioning | en_HK |
dc.subject | ATP-sensitive potassium channel | en_HK |
dc.subject | Intracellular Ca2+ | en_HK |
dc.subject | U50,488H | en_HK |
dc.title | Roles of KATP channels in delayed cardioprotection and intracellular Ca2+ in the rat heart as revealed by κ-opioid receptor stimulation with U50,488H | en_HK |
dc.type | Article | en_HK |
dc.identifier.email | Chen, M: chenmai@hotmail.com | en_HK |
dc.identifier.email | Kam, KWL: kam_wan_lung@hotmail.com | en_HK |
dc.identifier.email | Yan, WY: yanyi@e-garfield.com | en_HK |
dc.identifier.email | Wu, S: swua@hkucc.hku.hk | en_HK |
dc.identifier.email | Wong, TM: wongtakm@hkucc.hku.hk | en_HK |
dc.description.nature | link_to_OA_fulltext | en_HK |
dc.identifier.doi | 10.1038/sj.bjp.0705475 | en_HK |
dc.identifier.pmid | 14534156 | en_HK |
dc.identifier.pmcid | PMC1574065 | en_HK |
dc.identifier.scopus | eid_2-s2.0-0242660884 | - |
dc.identifier.volume | 140 | - |
dc.identifier.issue | 4 | - |
dc.identifier.spage | 750 | - |
dc.identifier.epage | 758 | - |
dc.identifier.isi | WOS:000186240600020 | - |
dc.identifier.issnl | 0007-1188 | - |