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Article: Development of T-tubular vacuoles in eccentrically damaged mouse muscle fibres

TitleDevelopment of T-tubular vacuoles in eccentrically damaged mouse muscle fibres
Authors
Issue Date2002
PublisherWiley-Blackwell Publishing Ltd.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0022-3751
Citation
Journal Of Physiology, 2002, v. 540 n. 2, p. 581-592 How to Cite?
AbstractSingle fibres were dissected from mouse flexor digitorum brevis muscles and subjected to a protocol of eccentric stretches consisting of ten tetani each with a 40 % stretch. Ten minutes later the fibres showed a reduced force, a shift in the peak of the force-length relation and a steepening of the force-frequency relation. Addition of the fluorescent dye sulforhodamine B to the extracellular space enabled the T-tubular system to be visualized. In unstimulated fibres and fibres subjected to 10 isometric tetani, the T-tubules were clearly delineated. Sulforhodamine B diffused out of the T-tubules with a half-time of 18 ± 1 s. Following the eccentric protocol, vacuoles connected to the T-tubules were detected in six out of seven fibres. Sulforhodamine B diffused out of the vacuoles of eccentrically damaged fibres extremely slowly with a half-time of 6.3 ± 2.4 min and diffused out of the T-tubules with a half-time of 39 ± 4 s. Vacuole production was eliminated by application of 1 mm ouabain to the muscle during the eccentric protocol. On removal of the ouabain, vacuoles appeared over a period of 1 h and were more numerous and more widely distributed than in the absence of ouabain. We propose that T-tubules are liable to rupture during eccentric contraction probably because of the relative movement associated with the inhomogeneity of sarcomere lengths. Such rupture raises intracellular sodium and when the sodium is pumped from the cell by the sodium pump, the volume load of Na+ and water exceeds the capacity of the T-tubules and causes vacuole production. The damage to the T-tubules may underlie a number of the functional changes that occur in eccentrically damaged muscle fibres.
Persistent Identifierhttp://hdl.handle.net/10722/49305
ISSN
2015 Impact Factor: 4.731
2015 SCImago Journal Rankings: 2.670
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYeung, EWen_HK
dc.contributor.authorBalnave, CDen_HK
dc.contributor.authorBallard, HJen_HK
dc.contributor.authorBourreau, JPen_HK
dc.contributor.authorAllen, DGen_HK
dc.date.accessioned2008-06-12T06:38:57Z-
dc.date.available2008-06-12T06:38:57Z-
dc.date.issued2002en_HK
dc.identifier.citationJournal Of Physiology, 2002, v. 540 n. 2, p. 581-592en_HK
dc.identifier.issn0022-3751en_HK
dc.identifier.urihttp://hdl.handle.net/10722/49305-
dc.description.abstractSingle fibres were dissected from mouse flexor digitorum brevis muscles and subjected to a protocol of eccentric stretches consisting of ten tetani each with a 40 % stretch. Ten minutes later the fibres showed a reduced force, a shift in the peak of the force-length relation and a steepening of the force-frequency relation. Addition of the fluorescent dye sulforhodamine B to the extracellular space enabled the T-tubular system to be visualized. In unstimulated fibres and fibres subjected to 10 isometric tetani, the T-tubules were clearly delineated. Sulforhodamine B diffused out of the T-tubules with a half-time of 18 ± 1 s. Following the eccentric protocol, vacuoles connected to the T-tubules were detected in six out of seven fibres. Sulforhodamine B diffused out of the vacuoles of eccentrically damaged fibres extremely slowly with a half-time of 6.3 ± 2.4 min and diffused out of the T-tubules with a half-time of 39 ± 4 s. Vacuole production was eliminated by application of 1 mm ouabain to the muscle during the eccentric protocol. On removal of the ouabain, vacuoles appeared over a period of 1 h and were more numerous and more widely distributed than in the absence of ouabain. We propose that T-tubules are liable to rupture during eccentric contraction probably because of the relative movement associated with the inhomogeneity of sarcomere lengths. Such rupture raises intracellular sodium and when the sodium is pumped from the cell by the sodium pump, the volume load of Na+ and water exceeds the capacity of the T-tubules and causes vacuole production. The damage to the T-tubules may underlie a number of the functional changes that occur in eccentrically damaged muscle fibres.en_HK
dc.format.extent388 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherWiley-Blackwell Publishing Ltd.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0022-3751en_HK
dc.relation.ispartofJournal of Physiologyen_HK
dc.rightsThe Journal of Physiology. Copyright © Blackwell Publishing Ltd.en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsThe definitive version is available at www.blackwell-synergy.comen_HK
dc.subject.meshMuscle Fibers - enzymology - pathologyen_HK
dc.subject.meshMuscle, Skeletal - enzymology - pathologyen_HK
dc.subject.meshVacuoles - enzymology - pathologyen_HK
dc.subject.meshEnzyme Inhibitors - pharmacologyen_HK
dc.subject.meshExtracellular Space - physiologyen_HK
dc.titleDevelopment of T-tubular vacuoles in eccentrically damaged mouse muscle fibresen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-3751&volume=540&issue=2&spage=581&epage=592&date=2002&atitle=Development+of+T-tubular+vacuoles+in+eccentrically-damaged+mouse+muscle+fibresen_HK
dc.identifier.emailBallard, HJ: ballard@hkucc.hku.hken_HK
dc.identifier.emailBourreau, JP: bourreau@hkucc.hku.hken_HK
dc.identifier.authorityBallard, HJ=rp00367en_HK
dc.identifier.authorityBourreau, JP=rp00389en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1113/jphysiol.2001.013839en_HK
dc.identifier.pmid11956345en_HK
dc.identifier.pmcidPMC2290255en_HK
dc.identifier.scopuseid_2-s2.0-0037092283en_HK
dc.identifier.hkuros72908-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037092283&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume540en_HK
dc.identifier.issue2en_HK
dc.identifier.spage581en_HK
dc.identifier.epage592en_HK
dc.identifier.isiWOS:000175262900015-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridYeung, EW=7101971748en_HK
dc.identifier.scopusauthoridBalnave, CD=6602214583en_HK
dc.identifier.scopusauthoridBallard, HJ=7005286310en_HK
dc.identifier.scopusauthoridBourreau, JP=7003927886en_HK
dc.identifier.scopusauthoridAllen, DG=35509336700en_HK

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