File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: 2-[125I]iodomelatonin-binding sites in the human kidney and the effect of guanosine 5'-0-(3-thiotriphosphate)

Title2-[125I]iodomelatonin-binding sites in the human kidney and the effect of guanosine 5'-0-(3-thiotriphosphate)
Authors
Issue Date1995
PublisherThe Endocrine Society. The Journal's web site is located at http://jcem.endojournals.org
Citation
Journal of Clinical Endocrinology and Metabolism, 1995, v. 80 n. 5, p. 1560-1565 How to Cite?
Abstract
Putative melatonin receptors in normal kidney cortical tissues of patients with transitional cell carcinoma or renal cell carcinoma were characterized using a melatonin agonist, 2-[125I]iodomelatonin, as the radioligand. 2-[125I]Iodomelatonin-binding sites in the human kidney were stable, saturable, reversible, and of high affinity. The binding affinity was 15.2 +/- 2.5 pmol/L, and the binding density was 1.79 +/- 0.19 fmol/mg protein. The unity of the Hill coefficients and linearity of the Scatchard plots suggested that 2-[125I]iodomelatonin was bound to a single class of binding sites. Pharmacological characterization showed that these binding sites were highly specific to 2-iodomelatonin, melatonin, 6-hydroxymelatonin, and 6-chloromelatonin. Guanosine 5'-O-(3-thiotriphosphate) decreased the binding affinity and density of 2-[125I]iodomelatonin-binding sites in the kidney, suggesting that these binding sites are coupled to a G-protein. The characterization of 2-[125I]iodomelatonin-binding sites in normal kidney tissues taken from patients with transitional cell carcinoma or renal cell carcinoma suggests the existence of 2-[125I]iodomelatonin-binding sites in the human kidney cortex, which is in line with the findings of 2-[125I]iodomelatonin-binding sites in kidneys of other mammals and birds. The implication of a direct melatonin action on renal function in the human is proposed.
Persistent Identifierhttp://hdl.handle.net/10722/49294
ISSN
2013 Impact Factor: 6.310
2013 SCImago Journal Rankings: 3.169
ISI Accession Number ID

 

Author Affiliations
  1. The University of Hong Kong
  2. Centre for Addiction and Mental Health
  3. Queen Mary Hospital Hong Kong
DC FieldValueLanguage
dc.contributor.authorSong, Yen_HK
dc.contributor.authorTam, PCen_HK
dc.contributor.authorPoon, AMSen_HK
dc.contributor.authorBrown, GMen_HK
dc.contributor.authorPang, SFen_HK
dc.date.accessioned2008-06-12T06:38:43Z-
dc.date.available2008-06-12T06:38:43Z-
dc.date.issued1995en_HK
dc.identifier.citationJournal of Clinical Endocrinology and Metabolism, 1995, v. 80 n. 5, p. 1560-1565en_HK
dc.identifier.issn0021-972Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/49294-
dc.description.abstractPutative melatonin receptors in normal kidney cortical tissues of patients with transitional cell carcinoma or renal cell carcinoma were characterized using a melatonin agonist, 2-[125I]iodomelatonin, as the radioligand. 2-[125I]Iodomelatonin-binding sites in the human kidney were stable, saturable, reversible, and of high affinity. The binding affinity was 15.2 +/- 2.5 pmol/L, and the binding density was 1.79 +/- 0.19 fmol/mg protein. The unity of the Hill coefficients and linearity of the Scatchard plots suggested that 2-[125I]iodomelatonin was bound to a single class of binding sites. Pharmacological characterization showed that these binding sites were highly specific to 2-iodomelatonin, melatonin, 6-hydroxymelatonin, and 6-chloromelatonin. Guanosine 5'-O-(3-thiotriphosphate) decreased the binding affinity and density of 2-[125I]iodomelatonin-binding sites in the kidney, suggesting that these binding sites are coupled to a G-protein. The characterization of 2-[125I]iodomelatonin-binding sites in normal kidney tissues taken from patients with transitional cell carcinoma or renal cell carcinoma suggests the existence of 2-[125I]iodomelatonin-binding sites in the human kidney cortex, which is in line with the findings of 2-[125I]iodomelatonin-binding sites in kidneys of other mammals and birds. The implication of a direct melatonin action on renal function in the human is proposed.en_HK
dc.format.extent418 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherThe Endocrine Society. The Journal's web site is located at http://jcem.endojournals.orgen_HK
dc.rightsJournal of Clinical Endocrinology and Metabolism. Copyright © The Endocrine Society.en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshGuanosine 5'-O-(3-thiotriphosphate) - pharmacologyen_HK
dc.subject.meshKidney cortex - metabolismen_HK
dc.subject.meshMelatonin - analogs & derivatives - metabolism - pharmacokineticsen_HK
dc.subject.meshCarcinoma, renal cell - metabolismen_HK
dc.subject.meshCarcinoma, transitional cell - metabolismen_HK
dc.title2-[125I]iodomelatonin-binding sites in the human kidney and the effect of guanosine 5'-0-(3-thiotriphosphate)en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-972X&volume=80&issue=5&spage=1560&epage=1565&date=1995&atitle=2-[125I]iodomelatonin-binding+sites+in+the+human+kidney+and+the+effect+of+guanosine+5%27-0-(3-thiotriphosphate)en_HK
dc.identifier.emailTam, PC: hrmstpc@HKUCC.hku.hken_HK
dc.identifier.emailPoon, AMS: amspoon@hkucc.hku.hken_HK
dc.identifier.emailPang, SF: hrmypsf@hkucc.hku.hken_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.pmid7745000en_HK
dc.identifier.scopuseid_2-s2.0-0028989283-
dc.identifier.hkuros2701-
dc.identifier.isiWOS:A1995QW61100020-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats