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Article: Characterization of alpha 2 adrenoceptors and other adrenoceptors in membranes isolated from dog mesenteric nerve axons

TitleCharacterization of alpha 2 adrenoceptors and other adrenoceptors in membranes isolated from dog mesenteric nerve axons
Authors
Issue Date1995
PublisherAmerican Society for Pharmacology and Experimental Therapeutics. The Journal's web site is located at http://jpet.aspetjournals.org
Citation
Journal of Pharmacology and Experimental Therapeutics, 1995, v. 275 n. 2, p. 978-986 How to Cite?
AbstractThe axonal membranes from dog mesenteric nerves were isolated and characterized. They had a high density of [3H]saxitoxin, [3H]rauwolscine and [125I]iodocyanopindolol binding sites, but low densities of [125I] omega-conotoxin (GVIA) and [3H]prazosin binding sites. The microsomal membranes had one of the higher densities of [3H]rauwolscine binding sites in centrifugation fractions. These sites were characterized by saturation, kinetic and competition studies. In competition with oxymetazoline, WB 4101, prazosin, ARC-239, SK&F 104078 and SK&F 104856, these rauwolscine binding sites were alpha 2A-like. Chloroethylclonidine pretreatment did not affect the ability of SK&F 104078 or SK&F 104856 to compete with rauwolscine binding sites on axonal membranes. Bmax values were unchanged by chloroethylclonidine under conditions that also failed to reduce Bmax values of rauwolscine binding to membranes from human platelets. These receptors appear to be alpha 2A adrenoceptors and may represent sites of prejunctional control of neurotransmitter release in the dog. This membrane preparation may be useful in biochemical studies of prejunctional neural receptors.
Persistent Identifierhttp://hdl.handle.net/10722/49287
ISSN
2015 Impact Factor: 3.76
2015 SCImago Journal Rankings: 1.847
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorDaniel, EEen_HK
dc.contributor.authorGaspar, Ven_HK
dc.contributor.authorKwan, CYen_HK
dc.date.accessioned2008-06-12T06:38:35Z-
dc.date.available2008-06-12T06:38:35Z-
dc.date.issued1995en_HK
dc.identifier.citationJournal of Pharmacology and Experimental Therapeutics, 1995, v. 275 n. 2, p. 978-986en_HK
dc.identifier.issn0022-3565en_HK
dc.identifier.urihttp://hdl.handle.net/10722/49287-
dc.description.abstractThe axonal membranes from dog mesenteric nerves were isolated and characterized. They had a high density of [3H]saxitoxin, [3H]rauwolscine and [125I]iodocyanopindolol binding sites, but low densities of [125I] omega-conotoxin (GVIA) and [3H]prazosin binding sites. The microsomal membranes had one of the higher densities of [3H]rauwolscine binding sites in centrifugation fractions. These sites were characterized by saturation, kinetic and competition studies. In competition with oxymetazoline, WB 4101, prazosin, ARC-239, SK&F 104078 and SK&F 104856, these rauwolscine binding sites were alpha 2A-like. Chloroethylclonidine pretreatment did not affect the ability of SK&F 104078 or SK&F 104856 to compete with rauwolscine binding sites on axonal membranes. Bmax values were unchanged by chloroethylclonidine under conditions that also failed to reduce Bmax values of rauwolscine binding to membranes from human platelets. These receptors appear to be alpha 2A adrenoceptors and may represent sites of prejunctional control of neurotransmitter release in the dog. This membrane preparation may be useful in biochemical studies of prejunctional neural receptors.en_HK
dc.format.extent418 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherAmerican Society for Pharmacology and Experimental Therapeutics. The Journal's web site is located at http://jpet.aspetjournals.orgen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshAxons - chemistryen_HK
dc.subject.meshMesenteric Arteries - innervationen_HK
dc.subject.meshReceptors, Adrenergic, alpha-2 - metabolismen_HK
dc.subject.meshAdrenergic alpha-Agonists - metabolismen_HK
dc.subject.meshAdrenergic alpha-Antagonists - metabolismen_HK
dc.titleCharacterization of alpha 2 adrenoceptors and other adrenoceptors in membranes isolated from dog mesenteric nerve axonsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-3565&volume=275&issue=2&spage=978&epage=986&date=1995&atitle=Characterization+of+alpha+2+adrenoceptors+and+other+adrenoceptors+in+membranes+isolated+from+dog+mesenteric+nerve+axonsen_HK
dc.identifier.emailKwan, CY: cykwan@hkucc.hku.hken_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.pmid7473191en_HK
dc.identifier.hkuros11244-
dc.identifier.isiWOS:A1995TE74300057-

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