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Article: Melatonin inhibits nitrate tolerance in isolated coronary arteries

TitleMelatonin inhibits nitrate tolerance in isolated coronary arteries
Authors
KeywordsCoronary arteries
Melatonin
Melatonin receptors
Nitrate tolerance
Nitroglycerin
Issue Date2003
PublisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0007-1188&site=1
Citation
British Journal Of Pharmacology, 2003, v. 139 n. 7, p. 1326-1332 How to Cite?
Abstract1. The present study was designed to test the hypothesis that melatonin inhibits nitrate tolerance in coronary arteries. 2. Rings of porcine coronary arteries were suspended in organ chambers for isometric tension recording. Nitrate tolerance was induced by incubating the tissues with nitroglycerin (10 -4 M) for 90 min, followed by repeated rinsing for 1 h. Control rings that had not been exposed previously to nitroglycerin, but were otherwise treated identically, were studied simultaneously. The rings were contracted with U46619 (1-3 x 10 -9 M) and concentration-response curves to nitroglycerin (10 -9-10 -4 M) were obtained. 3. Nitrate tolerance was evident by a 15- to 20-fold rightward shift in the concentration-response curve to nitroglycerin in rings with and without endothelium exposed previously to the drug for 90min. Addition of melatonin (10 -9 - 10 -7 M) to the organ chamber during the 90-min incubation period with nitroglycerin partially inhibited nitrate tolerance in coronary arteries with intact endothelium; however, melatonin had no effect on nitrate tolerance in coronary arteries without endothelium. 4. The effect of melatonin on nitrate tolerance in coronary arteries with endothelium was abolished by the melatonin receptor antagonist, S20928 (10 -6 M). In contrast to melatonin, the selective MT 3-melatonin receptor agonist, 5-MCA-NAT (10 -8-10 -7 M), had no effect on nitrate tolerance in coronary arteries. 5. The results demonstrate that melatonin, acting via specific melatonin receptors, inhibits nitrate tolerance in coronary arteries and that this effect is dependent on the presence of the vascular endothelium.
Persistent Identifierhttp://hdl.handle.net/10722/49280
ISSN
2015 Impact Factor: 5.259
2015 SCImago Journal Rankings: 2.368
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorO'Rourke, STen_HK
dc.contributor.authorHammad, Hen_HK
dc.contributor.authorDelagrange, Pen_HK
dc.contributor.authorScalbert, Een_HK
dc.contributor.authorVanhoutte, PMen_HK
dc.date.accessioned2008-06-12T06:38:22Z-
dc.date.available2008-06-12T06:38:22Z-
dc.date.issued2003en_HK
dc.identifier.citationBritish Journal Of Pharmacology, 2003, v. 139 n. 7, p. 1326-1332en_HK
dc.identifier.issn0007-1188en_HK
dc.identifier.urihttp://hdl.handle.net/10722/49280-
dc.description.abstract1. The present study was designed to test the hypothesis that melatonin inhibits nitrate tolerance in coronary arteries. 2. Rings of porcine coronary arteries were suspended in organ chambers for isometric tension recording. Nitrate tolerance was induced by incubating the tissues with nitroglycerin (10 -4 M) for 90 min, followed by repeated rinsing for 1 h. Control rings that had not been exposed previously to nitroglycerin, but were otherwise treated identically, were studied simultaneously. The rings were contracted with U46619 (1-3 x 10 -9 M) and concentration-response curves to nitroglycerin (10 -9-10 -4 M) were obtained. 3. Nitrate tolerance was evident by a 15- to 20-fold rightward shift in the concentration-response curve to nitroglycerin in rings with and without endothelium exposed previously to the drug for 90min. Addition of melatonin (10 -9 - 10 -7 M) to the organ chamber during the 90-min incubation period with nitroglycerin partially inhibited nitrate tolerance in coronary arteries with intact endothelium; however, melatonin had no effect on nitrate tolerance in coronary arteries without endothelium. 4. The effect of melatonin on nitrate tolerance in coronary arteries with endothelium was abolished by the melatonin receptor antagonist, S20928 (10 -6 M). In contrast to melatonin, the selective MT 3-melatonin receptor agonist, 5-MCA-NAT (10 -8-10 -7 M), had no effect on nitrate tolerance in coronary arteries. 5. The results demonstrate that melatonin, acting via specific melatonin receptors, inhibits nitrate tolerance in coronary arteries and that this effect is dependent on the presence of the vascular endothelium.en_HK
dc.format.extent388 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0007-1188&site=1en_HK
dc.relation.ispartofBritish Journal of Pharmacologyen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectCoronary arteriesen_HK
dc.subjectMelatoninen_HK
dc.subjectMelatonin receptorsen_HK
dc.subjectNitrate toleranceen_HK
dc.subjectNitroglycerinen_HK
dc.titleMelatonin inhibits nitrate tolerance in isolated coronary arteriesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0007-1188&volume=139&issue=7&spage=1326&epage=1332&date=2003&atitle=Melatonin+inhibits+nitrate+tolerance+in+isolated+coronary+arteries+en_HK
dc.identifier.emailVanhoutte, PM: vanhoutt@hku.hken_HK
dc.identifier.authorityVanhoutte, PM=rp00238en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1038/sj.bjp.0705383en_HK
dc.identifier.pmid12890712-
dc.identifier.pmcidPMC1573966en_HK
dc.identifier.scopuseid_2-s2.0-0041886518en_HK
dc.identifier.hkuros95750-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0041886518&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume139en_HK
dc.identifier.issue7en_HK
dc.identifier.spage1326en_HK
dc.identifier.epage1332en_HK
dc.identifier.isiWOS:000184553200011-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridO'Rourke, ST=7005313078en_HK
dc.identifier.scopusauthoridHammad, H=7003667911en_HK
dc.identifier.scopusauthoridDelagrange, P=7007022477en_HK
dc.identifier.scopusauthoridScalbert, E=7003716133en_HK
dc.identifier.scopusauthoridVanhoutte, PM=7202304247en_HK

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