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Article: Homocysteine stimulates nuclear factor kappaB activity and monocyte chemoattractant protein-1 expression in vascular smooth-muscle cells: a possible role for protein kinase C

TitleHomocysteine stimulates nuclear factor kappaB activity and monocyte chemoattractant protein-1 expression in vascular smooth-muscle cells: a possible role for protein kinase C
Authors
KeywordsAtherosclerosis
Calcium
Oxidative stress
Phosphorylation
Issue Date2000
PublisherPortland Press Ltd.
Citation
Biochemical Journal, 2000, v. 352 n. pt 3, p. 817-826 How to Cite?
AbstractMonocyte chemoattractant protein-1 (MCP-1) is a potent chemokine that stimulates the migration of monocytes into the intima of arterial walls. Although many factors that induce MCP-1 expression have been identified, the effect of homocysteine on the expression of MCP-1 in atherogenesis and the underlying mechanisms are not entirely clear. The objective of the present study was to investigate the role of homocysteine in MCP-1 expression in human aorta vascular smooth-muscle cells (VSMCs). After VSMCs were incubated with homocysteine for various time periods, a nuclease protection assay and ELISA were performed. Homocysteine (0.05-0.2 mM) significantly increased the expression of MCP-1 mRNA (up to 2. 7-fold) and protein (up to 3.3-fold) in these cells. The increase in MCP-1 expression was associated with the activation of protein kinase C (PKC) as well as nuclear factor kappaB (NF-kappaB). Further investigation demonstrated that the activation of NF-kappaB was the result of a PKC-mediated reduction in the expression of inhibitory protein (IkappaBalpha) mRNA and protein in homocysteine-treated cells. Oxidative stress might also be involved in the activation of NF-kappaB by homocysteine in VSMCs. In conclusion, the present study has clearly demonstrated that the activation of PKC as well as superoxide production followed by activation of NF-kappaB is responsible for homocysteine-induced MCP-1 expression in VSMCs. These results suggest that homocysteine-stimulated MCP-1 expression via NF-kappaB activation may play an important role in atherogenesis.
Persistent Identifierhttp://hdl.handle.net/10722/49279
ISSN
2015 Impact Factor: 3.562
2015 SCImago Journal Rankings: 2.582
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWang, Gen_HK
dc.contributor.authorSiow, YLen_HK
dc.contributor.authorO, Ken_HK
dc.date.accessioned2008-06-12T06:38:21Z-
dc.date.available2008-06-12T06:38:21Z-
dc.date.issued2000en_HK
dc.identifier.citationBiochemical Journal, 2000, v. 352 n. pt 3, p. 817-826en_HK
dc.identifier.issn0264-6021en_HK
dc.identifier.urihttp://hdl.handle.net/10722/49279-
dc.description.abstractMonocyte chemoattractant protein-1 (MCP-1) is a potent chemokine that stimulates the migration of monocytes into the intima of arterial walls. Although many factors that induce MCP-1 expression have been identified, the effect of homocysteine on the expression of MCP-1 in atherogenesis and the underlying mechanisms are not entirely clear. The objective of the present study was to investigate the role of homocysteine in MCP-1 expression in human aorta vascular smooth-muscle cells (VSMCs). After VSMCs were incubated with homocysteine for various time periods, a nuclease protection assay and ELISA were performed. Homocysteine (0.05-0.2 mM) significantly increased the expression of MCP-1 mRNA (up to 2. 7-fold) and protein (up to 3.3-fold) in these cells. The increase in MCP-1 expression was associated with the activation of protein kinase C (PKC) as well as nuclear factor kappaB (NF-kappaB). Further investigation demonstrated that the activation of NF-kappaB was the result of a PKC-mediated reduction in the expression of inhibitory protein (IkappaBalpha) mRNA and protein in homocysteine-treated cells. Oxidative stress might also be involved in the activation of NF-kappaB by homocysteine in VSMCs. In conclusion, the present study has clearly demonstrated that the activation of PKC as well as superoxide production followed by activation of NF-kappaB is responsible for homocysteine-induced MCP-1 expression in VSMCs. These results suggest that homocysteine-stimulated MCP-1 expression via NF-kappaB activation may play an important role in atherogenesis.en_HK
dc.format.extent388 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherPortland Press Ltd.en_HK
dc.rightsThe final version of record is available at http://www.biochemj.orgen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectAtherosclerosisen_HK
dc.subjectCalciumen_HK
dc.subjectOxidative stressen_HK
dc.subjectPhosphorylationen_HK
dc.titleHomocysteine stimulates nuclear factor kappaB activity and monocyte chemoattractant protein-1 expression in vascular smooth-muscle cells: a possible role for protein kinase Cen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0264-6021&volume=352&issue=pt 3&spage=817&epage=826&date=2000&atitle=Homocysteine+stimulates+nuclear+factor+kappaB+activity+and+monocyte+chemoattractant+protein-1+expression+in+vascular+smooth-muscle+cells:+a+possible+role+for+protein+kinase+Cen_HK
dc.identifier.emailSiow, YL: cylsiow@hkusua.hku.hken_HK
dc.identifier.emailO, K: okarmin@hkucc.hku.hken_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.pmid11104691en_HK
dc.identifier.pmcidPMC1221522en_HK
dc.identifier.hkuros61256-
dc.identifier.isiWOS:000166177700029-

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