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Article: Biosynthesis of plasmenylethanolamine (1-O-alk-1'-enyl-2-acyl-sn-glycero-3-phosphoethanolamine) in the guinea pig heart

TitleBiosynthesis of plasmenylethanolamine (1-O-alk-1'-enyl-2-acyl-sn-glycero-3-phosphoethanolamine) in the guinea pig heart
Authors
KeywordsPlasmenylethanolamine
Phosphatidylethanolamine
Diradylglycerol
Biosynthesis
Ethanolamine phosphotransferase
Issue Date1997
PublisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jlr.org/
Citation
Journal of Lipid Research, 1997, v. 38 n. 4, p. 670-679 How to Cite?
AbstractIn this study, the isolated guinea pig heart was pulse-labeled with a precursor of ethanolamine glycerophospholipid, and then chased with the non-radioactive compound for 0-8 h. Labeling with hexadecanol revealed that plasmanylethanolamine was the immediate precursor of plasmenylethanolamine, but a substantial portion of the label was also found in phosphatidylethanolamine. When ethanolamine was used as the precursor, the labeling of plasmenylethanolamine was between 50-65% of the labeling of phosphatidylethanolamine, and this ratio was maintained throughout the perfusion. The ratio of labeling is similar to the ratio of pool sizes of these ethanolamine glycerophospholipid in the heart, which implies that the CDP-ethanolamine pathway is also important for plasmenylethanolamine biosynthesis. The role of diradylglycerol in the synthesis of each ethanolamine glycerophospholipid was also investigated. The ratio of 1-alkenyl-2-acyl glycerol to total diradylglycerol content was 7% in the homogenate and 32% in the microsomes. However, ethanolamine phosphotransferase displayed a distinct selectivity towards 1-alkenyl-2-acyl glycerol. Kinetic studies revealed that the synthesis of phosphatidylethanolamine was inhibited by 1-alkenyl-2-acyl glycerol, but the formation of plasmenylethanolamine was not affected by 1,2-diacylglycerol. In addition, the inhibition of ethanolamine phosphotransferase by 1-alkyl-2-acyl glycerol appears to be an important mechanism for the coordination of plasmenylethanolamine biosynthesis via the desaturase reaction and the CDP-ethanolamine pathway.
Persistent Identifierhttp://hdl.handle.net/10722/49274
ISSN
2015 Impact Factor: 4.368
2015 SCImago Journal Rankings: 2.529
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorXu, FYen_HK
dc.contributor.authorO, Ken_HK
dc.contributor.authorChoy, PCen_HK
dc.date.accessioned2008-06-12T06:38:14Z-
dc.date.available2008-06-12T06:38:14Z-
dc.date.issued1997en_HK
dc.identifier.citationJournal of Lipid Research, 1997, v. 38 n. 4, p. 670-679en_HK
dc.identifier.issn0022-2275en_HK
dc.identifier.urihttp://hdl.handle.net/10722/49274-
dc.description.abstractIn this study, the isolated guinea pig heart was pulse-labeled with a precursor of ethanolamine glycerophospholipid, and then chased with the non-radioactive compound for 0-8 h. Labeling with hexadecanol revealed that plasmanylethanolamine was the immediate precursor of plasmenylethanolamine, but a substantial portion of the label was also found in phosphatidylethanolamine. When ethanolamine was used as the precursor, the labeling of plasmenylethanolamine was between 50-65% of the labeling of phosphatidylethanolamine, and this ratio was maintained throughout the perfusion. The ratio of labeling is similar to the ratio of pool sizes of these ethanolamine glycerophospholipid in the heart, which implies that the CDP-ethanolamine pathway is also important for plasmenylethanolamine biosynthesis. The role of diradylglycerol in the synthesis of each ethanolamine glycerophospholipid was also investigated. The ratio of 1-alkenyl-2-acyl glycerol to total diradylglycerol content was 7% in the homogenate and 32% in the microsomes. However, ethanolamine phosphotransferase displayed a distinct selectivity towards 1-alkenyl-2-acyl glycerol. Kinetic studies revealed that the synthesis of phosphatidylethanolamine was inhibited by 1-alkenyl-2-acyl glycerol, but the formation of plasmenylethanolamine was not affected by 1,2-diacylglycerol. In addition, the inhibition of ethanolamine phosphotransferase by 1-alkyl-2-acyl glycerol appears to be an important mechanism for the coordination of plasmenylethanolamine biosynthesis via the desaturase reaction and the CDP-ethanolamine pathway.en_HK
dc.format.extent418 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jlr.org/en_HK
dc.rightsJournal of Lipid Research. Copyright © American Society for Biochemistry and Molecular Biology, Inc.en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectPlasmenylethanolamineen_HK
dc.subjectPhosphatidylethanolamineen_HK
dc.subjectDiradylglycerolen_HK
dc.subjectBiosynthesisen_HK
dc.subjectEthanolamine phosphotransferaseen_HK
dc.titleBiosynthesis of plasmenylethanolamine (1-O-alk-1'-enyl-2-acyl-sn-glycero-3-phosphoethanolamine) in the guinea pig hearten_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-2275&volume=38&issue=4&spage=670&epage=679&date=1997&atitle=Biosynthesis+of+plasmenylethanolamine+(1-O-alk-1%27-enyl-2-acyl-sn-glycero-3-phosphoethanolamine)+in+the+guinea+pig+hearten_HK
dc.identifier.emailO, K: okarmin@hkucc.hku.hken_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.pmid9144082en_HK
dc.identifier.hkuros32812-
dc.identifier.isiWOS:A1997WX10300005-

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