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Article: Monoclonal antibody-based antigen capture enzyme-linked immunosorbent assay reveals high sensitivity of the nucleocapsid protein in acute-phase sera of severe acute respiratory syndrome patients

TitleMonoclonal antibody-based antigen capture enzyme-linked immunosorbent assay reveals high sensitivity of the nucleocapsid protein in acute-phase sera of severe acute respiratory syndrome patients
Authors
Issue Date2005
PublisherAmerican Society for Microbiology.
Citation
Clinical And Diagnostic Laboratory Immunology, 2005, v. 12 n. 1, p. 135-140 How to Cite?
AbstractAccurate and timely diagnosis of severe acute respiratory syndrome coronavirus (SARS-CoV) infection is a critical step in preventing another global outbreak. In this study, 829 serum specimens were collected from 643 patients initially reported to be infected with SARS-CoV. The sera were tested for the N protein of SARS-CoV by using an antigen capture enzyme-linked immunosorbent assay (ELISA) based on monoclonal antibodies against the N protein of SARS-CoV and compared to 197 control serum samples from healthy donors and non-SARS febrile patients. The results of the N protein detection analysis were directly related to the serological analysis data. From 27 SARS patients who tested positive with the neutralization test, 100% of the 24 sera collected from 1 to 10 days after the onset of symptoms were positive for the N protein. N protein was not detected beyond day 11 in this group. The positive rates of N protein for sera collected at 1 to 5, 6 to 10, 11 to 15, and 16 to 20 days after the onset of symptoms for 414 samples from 298 serologically confirmed patients were 92.9, 69.8, 36.4, and 21.1%, respectively. For 294 sera from 248 serological test-negative patients, the rates were 25.6, 16.7, 9.3, and 0%, respectively. The N protein was not detected in 66 patients with cases of what was initially suspected to be SARS but serologically proven to be negative for SARS and in 197 serum samples from healthy donors and non-SARS febrile patients. The specificity of the assay was 100%. Furthermore, of 16 sera collected from four patients during the SARS recurrence in Guangzhou, 5 sera collected from 7 to 9 days after the onset of symptoms were positive for the N protein. N protein detection exhibited a high positive rate, 96 to 100%, between day 3 and day 5 after the onset of symptoms for 27 neutralization test-positive SARS patients and 298 serologically confirmed patients. The N protein detection rate continually decreased beginning with day 10, and N protein was not detected beyond day 19 after the onset of symptoms. In conclusion, an antigen capture ELISA reveals a high N protein detection rate in acute-phase sera of patients with SARS, which makes it useful for early diagnosis of SARS.
Persistent Identifierhttp://hdl.handle.net/10722/49261
ISSN
2007 Impact Factor: 2.511
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorDi, Ben_HK
dc.contributor.authorHao, Wen_HK
dc.contributor.authorGao, Yen_HK
dc.contributor.authorWang, Men_HK
dc.contributor.authorWang, YDen_HK
dc.contributor.authorQiu, LWen_HK
dc.contributor.authorWen, Ken_HK
dc.contributor.authorZhou, DHen_HK
dc.contributor.authorWu, XWen_HK
dc.contributor.authorLu, EJen_HK
dc.contributor.authorLiao, ZYen_HK
dc.contributor.authorMei, YBen_HK
dc.contributor.authorZheng, BJen_HK
dc.contributor.authorChe, XYen_HK
dc.date.accessioned2008-06-12T06:37:49Z-
dc.date.available2008-06-12T06:37:49Z-
dc.date.issued2005en_HK
dc.identifier.citationClinical And Diagnostic Laboratory Immunology, 2005, v. 12 n. 1, p. 135-140en_HK
dc.identifier.issn1071-412Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/49261-
dc.description.abstractAccurate and timely diagnosis of severe acute respiratory syndrome coronavirus (SARS-CoV) infection is a critical step in preventing another global outbreak. In this study, 829 serum specimens were collected from 643 patients initially reported to be infected with SARS-CoV. The sera were tested for the N protein of SARS-CoV by using an antigen capture enzyme-linked immunosorbent assay (ELISA) based on monoclonal antibodies against the N protein of SARS-CoV and compared to 197 control serum samples from healthy donors and non-SARS febrile patients. The results of the N protein detection analysis were directly related to the serological analysis data. From 27 SARS patients who tested positive with the neutralization test, 100% of the 24 sera collected from 1 to 10 days after the onset of symptoms were positive for the N protein. N protein was not detected beyond day 11 in this group. The positive rates of N protein for sera collected at 1 to 5, 6 to 10, 11 to 15, and 16 to 20 days after the onset of symptoms for 414 samples from 298 serologically confirmed patients were 92.9, 69.8, 36.4, and 21.1%, respectively. For 294 sera from 248 serological test-negative patients, the rates were 25.6, 16.7, 9.3, and 0%, respectively. The N protein was not detected in 66 patients with cases of what was initially suspected to be SARS but serologically proven to be negative for SARS and in 197 serum samples from healthy donors and non-SARS febrile patients. The specificity of the assay was 100%. Furthermore, of 16 sera collected from four patients during the SARS recurrence in Guangzhou, 5 sera collected from 7 to 9 days after the onset of symptoms were positive for the N protein. N protein detection exhibited a high positive rate, 96 to 100%, between day 3 and day 5 after the onset of symptoms for 27 neutralization test-positive SARS patients and 298 serologically confirmed patients. The N protein detection rate continually decreased beginning with day 10, and N protein was not detected beyond day 19 after the onset of symptoms. In conclusion, an antigen capture ELISA reveals a high N protein detection rate in acute-phase sera of patients with SARS, which makes it useful for early diagnosis of SARS.en_HK
dc.format.extent386 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherAmerican Society for Microbiology.en_HK
dc.relation.ispartofClinical and Diagnostic Laboratory Immunologyen_HK
dc.rightsClinical and Diagnostic Laboratory Immunology. Copyright © American Society for Microbiology.en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsCopyright © American Society for Microbiology, Clinical and Diagnostic Laboratory Immunology, 2005, v. 12 n. 1, p. 135-140en_HK
dc.subject.meshAntibodies, Monoclonal - diagnostic useen_HK
dc.subject.meshAntigens, Viral - blooden_HK
dc.subject.meshEnzyme-Linked Immunosorbent Assay - methodsen_HK
dc.subject.meshNucleocapsid Proteins - blood - immunologyen_HK
dc.subject.meshSevere Acute Respiratory Syndrome - blood - diagnosis - immunologyen_HK
dc.titleMonoclonal antibody-based antigen capture enzyme-linked immunosorbent assay reveals high sensitivity of the nucleocapsid protein in acute-phase sera of severe acute respiratory syndrome patientsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1071-412X&volume=12&issue=1&spage=135&epage=140&date=2005&atitle=Monoclonal+antibody-based+antigen+capture+enzyme-linked+immunosorbent+assay+reveals+high+sensitivity+of+the+nucleocapsid+protein+in+acute-phase+sera+of+severe+acute+respiratory+syndrome+patientsen_HK
dc.identifier.emailZheng, BJ:bzheng@hkucc.hku.hken_HK
dc.identifier.authorityZheng, BJ=rp00353en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1128/CDLI.12.1.135-140.2005en_HK
dc.identifier.pmid15642998-
dc.identifier.pmcidPMC540218en_HK
dc.identifier.scopuseid_2-s2.0-19944432784en_HK
dc.identifier.hkuros99118-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-19944432784&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume12en_HK
dc.identifier.issue1en_HK
dc.identifier.spage135en_HK
dc.identifier.epage140en_HK
dc.identifier.isiWOS:000227271800019-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridDi, B=6602190900en_HK
dc.identifier.scopusauthoridHao, W=7101686587en_HK
dc.identifier.scopusauthoridGao, Y=7404706103en_HK
dc.identifier.scopusauthoridWang, M=7406685232en_HK
dc.identifier.scopusauthoridWang, YD=9638471800en_HK
dc.identifier.scopusauthoridQiu, LW=8719102700en_HK
dc.identifier.scopusauthoridWen, K=8231190100en_HK
dc.identifier.scopusauthoridZhou, DH=7403395022en_HK
dc.identifier.scopusauthoridWu, XW=7407060805en_HK
dc.identifier.scopusauthoridLu, EJ=7102537056en_HK
dc.identifier.scopusauthoridLiao, ZY=7203032864en_HK
dc.identifier.scopusauthoridMei, YB=8231190400en_HK
dc.identifier.scopusauthoridZheng, BJ=7201780588en_HK
dc.identifier.scopusauthoridChe, XY=7005743182en_HK

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