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Article: Lytic viral replication as a contributor to the detection of Epstein-Barr virus in breast cancer

TitleLytic viral replication as a contributor to the detection of Epstein-Barr virus in breast cancer
Authors
Issue Date2003
PublisherAmerican Society for Microbiology.
Citation
Journal of Virology, 2003, v. 77 n. 24, p. 13267-13274 How to Cite?
AbstractEpstein-Barr virus (EBV) has an accepted association with the epithelial malignancy nasopharyngeal carcinoma and has also been reported in other more controversial carcinoma settings. Evaluation of EBV association with epithelial carcinomas such as breast cancer would benefit from a better understanding of the outcome of EBV infection of these cells. Cell-free preparations of a green fluorescent protein-expressing virus, BX1, were used to infect breast cancer cell lines, which were then examined for EBV gene expression and viral genome copy number. Reverse transcription-PCR analyses revealed that the cells supported a mixture of latency II and lytic EBV gene expression. Lytic Zta and BMRF1 protein expression was detected by immunohistochemistry, and DNA PCR analyses estimated an EBV copy number of 300 to 600 genomes per infected cell. Evidence for lytic EBV expression was also found in breast tissue, where reverse transcription-PCR analyses detected lytic Zta transcripts in 7 of 10 breast carcinoma tissues and 4 of 10 normal tissues from the same patients. Scattered cells immunoreactive for Zta protein were also detectable in breast carcinoma. Quantitative real-time PCR analysis of EBV-positive breast carcinoma tissues suggested that less than 0.1% of the cells contained viral genomes. We suggest that sporadic lytic EBV infection may contribute to PCR-based detection of EBV in traditionally nonvirally associated epithelial malignancies.
Persistent Identifierhttp://hdl.handle.net/10722/49250
ISSN
2015 Impact Factor: 4.606
2015 SCImago Journal Rankings: 3.347
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorHuang, Jen_HK
dc.contributor.authorChen, Hen_HK
dc.contributor.authorHutt-Fletcher, Len_HK
dc.contributor.authorAmbinder, RFen_HK
dc.contributor.authorHayward, SDen_HK
dc.date.accessioned2008-06-12T06:37:35Z-
dc.date.available2008-06-12T06:37:35Z-
dc.date.issued2003en_HK
dc.identifier.citationJournal of Virology, 2003, v. 77 n. 24, p. 13267-13274en_HK
dc.identifier.issn0022-538Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/49250-
dc.description.abstractEpstein-Barr virus (EBV) has an accepted association with the epithelial malignancy nasopharyngeal carcinoma and has also been reported in other more controversial carcinoma settings. Evaluation of EBV association with epithelial carcinomas such as breast cancer would benefit from a better understanding of the outcome of EBV infection of these cells. Cell-free preparations of a green fluorescent protein-expressing virus, BX1, were used to infect breast cancer cell lines, which were then examined for EBV gene expression and viral genome copy number. Reverse transcription-PCR analyses revealed that the cells supported a mixture of latency II and lytic EBV gene expression. Lytic Zta and BMRF1 protein expression was detected by immunohistochemistry, and DNA PCR analyses estimated an EBV copy number of 300 to 600 genomes per infected cell. Evidence for lytic EBV expression was also found in breast tissue, where reverse transcription-PCR analyses detected lytic Zta transcripts in 7 of 10 breast carcinoma tissues and 4 of 10 normal tissues from the same patients. Scattered cells immunoreactive for Zta protein were also detectable in breast carcinoma. Quantitative real-time PCR analysis of EBV-positive breast carcinoma tissues suggested that less than 0.1% of the cells contained viral genomes. We suggest that sporadic lytic EBV infection may contribute to PCR-based detection of EBV in traditionally nonvirally associated epithelial malignancies.en_HK
dc.format.extent386 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherAmerican Society for Microbiology.en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsJournal of Virology. Copyright © American Society for Microbiology.en_HK
dc.rightsCopyright © American Society for Microbiology, Journal of Virology, 2003, v. 77 n. 24, p. 13267-13274en_HK
dc.subject.meshBreast Neoplasms - virologyen_HK
dc.subject.meshHerpesvirus 4, Human - genetics - isolation & purification - pathogenicity - physiologyen_HK
dc.subject.meshViral Proteinsen_HK
dc.subject.meshVirus Replicationen_HK
dc.subject.meshAntigens, Viral - genetics - metabolismen_HK
dc.titleLytic viral replication as a contributor to the detection of Epstein-Barr virus in breast canceren_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-538X&volume=77&issue=24&spage=13267&epage=13274&date=2003&atitle=Lytic+viral+replication+as+a+contributor+to+the+detection+of+Epstein-Barr+virus+in+breast+canceren_HK
dc.identifier.emailChen, H: hlchen@hku.hken_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1128/JVI.77.24.13267-13274.2003en_HK
dc.identifier.pmid14645583-
dc.identifier.pmcidPMC296054en_HK
dc.identifier.scopuseid_2-s2.0-0344736829-
dc.identifier.hkuros88027-
dc.identifier.isiWOS:000187025500035-

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