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Article: Seronegative bacteremic melioidosis caused by Burkholderia pseudomallei with ambiguous biochemical profile: Clinical importance of accurate identification by 16S rRNA gene and groEL gene sequencing

TitleSeronegative bacteremic melioidosis caused by Burkholderia pseudomallei with ambiguous biochemical profile: Clinical importance of accurate identification by 16S rRNA gene and groEL gene sequencing
Authors
Issue Date2003
PublisherAmerican Society for Microbiology.
Citation
Journal Of Clinical Microbiology, 2003, v. 41 n. 8, p. 3973-3977 How to Cite?
AbstractAn aerobic gram-negative bacterium was isolated from the blood and sputum of an 84-year-old, chair-bound nursing home resident with acute bacteremic pneumonia. Although the phenotypic characteristics suggested that the bacterium could be Burkholderia pseudomallei, the Vitek 1 system (GNI+), which can successfully identify 99% of B. pseudomallei strains, showed that the bacterium was "unidentified." Immunoglobulin G against the lipopolysaccharide (LPS) of B. pseudomallei, as detected by an LPS-based enzyme-linked immunosorbent assay with 95% sensitivity, was negative in both the acute-phase and convalescent-phase sera. Sequencing of the groEL gene showed that the isolate was B. pseudomallei. Proper identification of the bacterium in this study is crucial, since there would be a radical difference in the duration of antimicrobial therapy.
Persistent Identifierhttp://hdl.handle.net/10722/49232
ISSN
2015 Impact Factor: 3.631
2015 SCImago Journal Rankings: 2.151
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWoo, PCYen_HK
dc.contributor.authorLau, SKPen_HK
dc.contributor.authorWoo, GKSen_HK
dc.contributor.authorFung, AMYen_HK
dc.contributor.authorNgan, AHYen_HK
dc.contributor.authorHui, WTen_HK
dc.contributor.authorYuen, KYen_HK
dc.date.accessioned2008-06-12T06:37:14Z-
dc.date.available2008-06-12T06:37:14Z-
dc.date.issued2003en_HK
dc.identifier.citationJournal Of Clinical Microbiology, 2003, v. 41 n. 8, p. 3973-3977en_HK
dc.identifier.issn0095-1137en_HK
dc.identifier.urihttp://hdl.handle.net/10722/49232-
dc.description.abstractAn aerobic gram-negative bacterium was isolated from the blood and sputum of an 84-year-old, chair-bound nursing home resident with acute bacteremic pneumonia. Although the phenotypic characteristics suggested that the bacterium could be Burkholderia pseudomallei, the Vitek 1 system (GNI+), which can successfully identify 99% of B. pseudomallei strains, showed that the bacterium was "unidentified." Immunoglobulin G against the lipopolysaccharide (LPS) of B. pseudomallei, as detected by an LPS-based enzyme-linked immunosorbent assay with 95% sensitivity, was negative in both the acute-phase and convalescent-phase sera. Sequencing of the groEL gene showed that the isolate was B. pseudomallei. Proper identification of the bacterium in this study is crucial, since there would be a radical difference in the duration of antimicrobial therapy.en_HK
dc.format.extent386 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherAmerican Society for Microbiology.en_HK
dc.relation.ispartofJournal of Clinical Microbiologyen_HK
dc.rightsJournal of Clinical Microbiology. Copyright © American Society for Microbiology.en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsCopyright © American Society for Microbiology, Journal of Clinical Microbiology, 2003, v. 41 n. 8, p. 3973-3977en_HK
dc.subject.meshBurkholderia pseudomallei - classification - genetics - isolation & purificationen_HK
dc.subject.meshGroEL Protein - geneticsen_HK
dc.subject.meshMelioidosis - diagnosisen_HK
dc.subject.meshRNA, Ribosomal, 16S - geneticsen_HK
dc.subject.meshDNA Primersen_HK
dc.titleSeronegative bacteremic melioidosis caused by Burkholderia pseudomallei with ambiguous biochemical profile: Clinical importance of accurate identification by 16S rRNA gene and groEL gene sequencingen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0095-1137&volume=41&issue=8&spage=3973&epage=3977&date=2003&atitle=Seronegative+bacteremic+melioidosis+caused+by+Burkholderia+pseudomallei+with+ambiguous+biochemical+profile:+clinical+importance+of+accurate+identification+by+16S+rRNA+gene+and+groEL+gene+sequencingen_HK
dc.identifier.emailWoo, PCY:pcywoo@hkucc.hku.hken_HK
dc.identifier.emailLau, SKP:skplau@hkucc.hku.hken_HK
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityWoo, PCY=rp00430en_HK
dc.identifier.authorityLau, SKP=rp00486en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1128/JCM.41.8.3973-3977.2003en_HK
dc.identifier.pmid12904433-
dc.identifier.pmcidPMC179777en_HK
dc.identifier.scopuseid_2-s2.0-0042022029en_HK
dc.identifier.hkuros80179-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0042022029&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume41en_HK
dc.identifier.issue8en_HK
dc.identifier.spage3973en_HK
dc.identifier.epage3977en_HK
dc.identifier.isiWOS:000184746500094-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridWoo, PCY=7201801340en_HK
dc.identifier.scopusauthoridLau, SKP=7401596211en_HK
dc.identifier.scopusauthoridWoo, GKS=7006485416en_HK
dc.identifier.scopusauthoridFung, AMY=7101926801en_HK
dc.identifier.scopusauthoridNgan, AHY=14037517900en_HK
dc.identifier.scopusauthoridHui, WT=7103196480en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK

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