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Article: Detection of antibodies specific to an antigenic cell wall galactomannoprotein for serodiagnosis of Apergillus fumigatus aspergillosis

TitleDetection of antibodies specific to an antigenic cell wall galactomannoprotein for serodiagnosis of Apergillus fumigatus aspergillosis
Authors
Issue Date2002
PublisherAmerican Society for Microbiology.
Citation
Journal Of Clinical Microbiology, 2002, v. 40 n. 6, p. 2041-2045 How to Cite?
AbstractAspergilloma and invasive aspergillosis are important opportunistic infections caused by Aspergillus species, among which Aspergillus fumigatus is the most common species associated with human disease. We developed an enzyme-linked immunosorbent assay (ELISA)-based antibody assay with Afmp1p, a purified recombinant antigenic cell wall galactomannoprotein of A. fumigatus. Evaluation of the test with guinea pig sera against A. fumigatus and other pathogenic fungi indicated that this assay was specific for A. fumigatus. Clinical evaluation revealed that the assay was 100% sensitive for patients with aspergilloma and 33.3% sensitive for patients with invasive aspergillosis. No false-positive results were found for serum samples from 80 healthy blood donors, 6 patients with typhoid fever, 4 patients with melioidosis, 20 patients with penicilliosis marneffei, 5 patients with candidiasis, and 4 patients with cryptococcosis, indicating a high specificity of the test. Thus, this ELISA-based test for the detection of anti-Afmp1p antibody can be of significant value as a diagnostic for aspergillosis.
Persistent Identifierhttp://hdl.handle.net/10722/49225
ISSN
2015 Impact Factor: 3.631
2015 SCImago Journal Rankings: 2.151
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChan, CMen_HK
dc.contributor.authorWoo, PCYen_HK
dc.contributor.authorLeung, ASPen_HK
dc.contributor.authorLau, SKPen_HK
dc.contributor.authorChe, XYen_HK
dc.contributor.authorCao, Len_HK
dc.contributor.authorYuen, KYen_HK
dc.date.accessioned2008-06-12T06:37:06Z-
dc.date.available2008-06-12T06:37:06Z-
dc.date.issued2002en_HK
dc.identifier.citationJournal Of Clinical Microbiology, 2002, v. 40 n. 6, p. 2041-2045en_HK
dc.identifier.issn0095-1137en_HK
dc.identifier.urihttp://hdl.handle.net/10722/49225-
dc.description.abstractAspergilloma and invasive aspergillosis are important opportunistic infections caused by Aspergillus species, among which Aspergillus fumigatus is the most common species associated with human disease. We developed an enzyme-linked immunosorbent assay (ELISA)-based antibody assay with Afmp1p, a purified recombinant antigenic cell wall galactomannoprotein of A. fumigatus. Evaluation of the test with guinea pig sera against A. fumigatus and other pathogenic fungi indicated that this assay was specific for A. fumigatus. Clinical evaluation revealed that the assay was 100% sensitive for patients with aspergilloma and 33.3% sensitive for patients with invasive aspergillosis. No false-positive results were found for serum samples from 80 healthy blood donors, 6 patients with typhoid fever, 4 patients with melioidosis, 20 patients with penicilliosis marneffei, 5 patients with candidiasis, and 4 patients with cryptococcosis, indicating a high specificity of the test. Thus, this ELISA-based test for the detection of anti-Afmp1p antibody can be of significant value as a diagnostic for aspergillosis.en_HK
dc.format.extent386 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherAmerican Society for Microbiology.en_HK
dc.relation.ispartofJournal of Clinical Microbiologyen_HK
dc.rightsJournal of Clinical Microbiology. Copyright © American Society for Microbiology.en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsCopyright © American Society for Microbiology, Journal of Clinical Microbiology, 2002, v. 40 n. 6, p. 2041-2045en_HK
dc.subject.meshAntibodies, Fungal - blooden_HK
dc.subject.meshAntibody Specificityen_HK
dc.subject.meshAntigens, Fungal - genetics - immunologyen_HK
dc.subject.meshAspergillosis - diagnosisen_HK
dc.subject.meshAspergillus fumigatus - immunologyen_HK
dc.titleDetection of antibodies specific to an antigenic cell wall galactomannoprotein for serodiagnosis of Apergillus fumigatus aspergillosisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0095-1137&volume=40&issue=6&spage=2041&epage=2045&date=2002&atitle=Detection+of+antibodies+specific+to+an+antigenic+cell+wall+galactomannoprotein+for+serodiagnosis+of+Aspergillus+fumigatus+aspergillosisen_HK
dc.identifier.emailWoo, PCY:pcywoo@hkucc.hku.hken_HK
dc.identifier.emailLau, SKP:skplau@hkucc.hku.hken_HK
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityWoo, PCY=rp00430en_HK
dc.identifier.authorityLau, SKP=rp00486en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1128/JCM.40.6.2041-2045.2002en_HK
dc.identifier.pmid12037061-
dc.identifier.pmcidPMC130809en_HK
dc.identifier.scopuseid_2-s2.0-0036263067en_HK
dc.identifier.hkuros74435-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036263067&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume40en_HK
dc.identifier.issue6en_HK
dc.identifier.spage2041en_HK
dc.identifier.epage2045en_HK
dc.identifier.isiWOS:000176159200023-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChan, CM=7404814453en_HK
dc.identifier.scopusauthoridWoo, PCY=7201801340en_HK
dc.identifier.scopusauthoridLeung, ASP=7403012546en_HK
dc.identifier.scopusauthoridLau, SKP=7401596211en_HK
dc.identifier.scopusauthoridChe, XY=7005743182en_HK
dc.identifier.scopusauthoridCao, L=7401637818en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK

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