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Article: Identification by 16S rRNA gene sequencing of Lactobacillus salivarius bacteremic cholecystitis

TitleIdentification by 16S rRNA gene sequencing of Lactobacillus salivarius bacteremic cholecystitis
Authors
Issue Date2002
PublisherAmerican Society for Microbiology.
Citation
Journal Of Clinical Microbiology, 2002, v. 40 n. 1, p. 265-267 How to Cite?
AbstractAn anaerobic, nonsporulating, gram-positive bacterium was isolated from blood and bile pus cultures of a 70-year-old man with bacteremic acute cholecystitis. The API 20A system showed that it was 70% Actinomyces naeslundii and 30% Bifidobacterium species, whereas the Vitek ANI system and the ATB ID32A Expression system showed that it was "unidentified." The 16S rRNA gene of the strain was amplified and sequenced. There were 3 base differences between the nucleotide sequence of the isolate and that of Lactobacillus salivarius subsp. salivarius or L. salivarius subsp. salicinius, indicating that the isolate was a strain of L. salivarius. The patient responded to cholecystectomy and a 2-week course of antibiotic treatment. Identification of the organism in the present study was important because the duration of antibiotic therapy would have been entirely different depending on the organism. If the bacterium had been identified as Actinomyces, penicillin for 6 months would have been the regimen of choice. However, it was Lactobacillus, and a 2-week course of antibiotic was sufficient.
Persistent Identifierhttp://hdl.handle.net/10722/49224
ISSN
2015 Impact Factor: 3.631
2015 SCImago Journal Rankings: 2.151
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWoo, PCYen_HK
dc.contributor.authorFung, AMYen_HK
dc.contributor.authorLau, SKPen_HK
dc.contributor.authorYuen, KYen_HK
dc.date.accessioned2008-06-12T06:37:05Z-
dc.date.available2008-06-12T06:37:05Z-
dc.date.issued2002en_HK
dc.identifier.citationJournal Of Clinical Microbiology, 2002, v. 40 n. 1, p. 265-267en_HK
dc.identifier.issn0095-1137en_HK
dc.identifier.urihttp://hdl.handle.net/10722/49224-
dc.description.abstractAn anaerobic, nonsporulating, gram-positive bacterium was isolated from blood and bile pus cultures of a 70-year-old man with bacteremic acute cholecystitis. The API 20A system showed that it was 70% Actinomyces naeslundii and 30% Bifidobacterium species, whereas the Vitek ANI system and the ATB ID32A Expression system showed that it was "unidentified." The 16S rRNA gene of the strain was amplified and sequenced. There were 3 base differences between the nucleotide sequence of the isolate and that of Lactobacillus salivarius subsp. salivarius or L. salivarius subsp. salicinius, indicating that the isolate was a strain of L. salivarius. The patient responded to cholecystectomy and a 2-week course of antibiotic treatment. Identification of the organism in the present study was important because the duration of antibiotic therapy would have been entirely different depending on the organism. If the bacterium had been identified as Actinomyces, penicillin for 6 months would have been the regimen of choice. However, it was Lactobacillus, and a 2-week course of antibiotic was sufficient.en_HK
dc.format.extent386 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherAmerican Society for Microbiology.en_HK
dc.relation.ispartofJournal of Clinical Microbiologyen_HK
dc.rightsJournal of Clinical Microbiology. Copyright © American Society for Microbiology.en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsCopyright © American Society for Microbiology, Journal of Clinical Microbiology, 2002, v. 40 n. 1, p. 265-267en_HK
dc.subject.meshBacteremia - microbiologyen_HK
dc.subject.meshCholecystitis - microbiologyen_HK
dc.subject.meshLactobacillus - classification - genetics - isolation & purificationen_HK
dc.subject.meshRNA, Ribosomal, 16S - geneticsen_HK
dc.subject.meshSequence Analysis, DNAen_HK
dc.titleIdentification by 16S rRNA gene sequencing of Lactobacillus salivarius bacteremic cholecystitisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0095-1137&volume=40&issue=1&spage=265&epage=267&date=2002&atitle=Identification+by+16S+rRNA+gene+sequencing+of+Lactobacillus+salivarius+bacteremic+cholecystitisen_HK
dc.identifier.emailWoo, PCY:pcywoo@hkucc.hku.hken_HK
dc.identifier.emailLau, SKP:skplau@hkucc.hku.hken_HK
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityWoo, PCY=rp00430en_HK
dc.identifier.authorityLau, SKP=rp00486en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1128/JCM.40.1.265-267.2002en_HK
dc.identifier.pmid11773128-
dc.identifier.pmcidPMC120105en_HK
dc.identifier.scopuseid_2-s2.0-0036135742en_HK
dc.identifier.hkuros74419-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036135742&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume40en_HK
dc.identifier.issue1en_HK
dc.identifier.spage265en_HK
dc.identifier.epage267en_HK
dc.identifier.isiWOS:000173121800046-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridWoo, PCY=7201801340en_HK
dc.identifier.scopusauthoridFung, AMY=7101926801en_HK
dc.identifier.scopusauthoridLau, SKP=7401596211en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK

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