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Article: Evaluation of the directigen FluA+B test for rapid diagnosis of influenza virus type A and B infections

TitleEvaluation of the directigen FluA+B test for rapid diagnosis of influenza virus type A and B infections
Authors
Issue Date2002
PublisherAmerican Society for Microbiology.
Citation
Journal Of Clinical Microbiology, 2002, v. 40 n. 5, p. 1675-1680 How to Cite?
AbstractDirectigen FluA+B (BD Diagnostic Systems, Sparks, Md.), a new rapid test for the detection of influenza virus types A and B, was evaluated with nasopharyngeal aspirate specimens collected from 250 patients in comparison with culture and direct fluorescent antigen (DFA) detection tests. The patients studied were predominantly children, 80% being ≤6 years old. Specimens negative by culture but positive by the Directigen FluA+B or DFA tests were analyzed by reverse transcription-PCR to resolve the discrepant results. The resolved sensitivity, specificity, and positive and negative predictive values of the Directigen FluA+B test for influenza virus type A were 96%, 99.6%, 96%, and 99.6%, respectively, and for influenza virus type B they were 87.5%, 96.8%, 80%, and 98%, respectively. Storage of nasopharyngeal aspirates in virus transport medium at 2 to 8°C for 48 h had little adverse effect on the detection of influenza virus type A, but diagnosis of influenza virus type B is best carried out with fresh specimens. The test detected a range of human and animal influenza virus A subtypes, including the H5N1 and H9N2 viruses that recently caused human disease in Hong Kong.
Persistent Identifierhttp://hdl.handle.net/10722/49214
ISSN
2015 Impact Factor: 3.631
2015 SCImago Journal Rankings: 2.151
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChan, KHen_HK
dc.contributor.authorMaldeis, Nen_HK
dc.contributor.authorPope, Wen_HK
dc.contributor.authorYup, Aen_HK
dc.contributor.authorOzinskas, Aen_HK
dc.contributor.authorGill, Jen_HK
dc.contributor.authorSeto, WHen_HK
dc.contributor.authorShortridge, KFen_HK
dc.contributor.authorPeiris, JSMen_HK
dc.date.accessioned2008-06-12T06:36:53Z-
dc.date.available2008-06-12T06:36:53Z-
dc.date.issued2002en_HK
dc.identifier.citationJournal Of Clinical Microbiology, 2002, v. 40 n. 5, p. 1675-1680en_HK
dc.identifier.issn0095-1137en_HK
dc.identifier.urihttp://hdl.handle.net/10722/49214-
dc.description.abstractDirectigen FluA+B (BD Diagnostic Systems, Sparks, Md.), a new rapid test for the detection of influenza virus types A and B, was evaluated with nasopharyngeal aspirate specimens collected from 250 patients in comparison with culture and direct fluorescent antigen (DFA) detection tests. The patients studied were predominantly children, 80% being ≤6 years old. Specimens negative by culture but positive by the Directigen FluA+B or DFA tests were analyzed by reverse transcription-PCR to resolve the discrepant results. The resolved sensitivity, specificity, and positive and negative predictive values of the Directigen FluA+B test for influenza virus type A were 96%, 99.6%, 96%, and 99.6%, respectively, and for influenza virus type B they were 87.5%, 96.8%, 80%, and 98%, respectively. Storage of nasopharyngeal aspirates in virus transport medium at 2 to 8°C for 48 h had little adverse effect on the detection of influenza virus type A, but diagnosis of influenza virus type B is best carried out with fresh specimens. The test detected a range of human and animal influenza virus A subtypes, including the H5N1 and H9N2 viruses that recently caused human disease in Hong Kong.en_HK
dc.format.extent392 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherAmerican Society for Microbiology.en_HK
dc.relation.ispartofJournal of Clinical Microbiologyen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsJournal of Clinical Microbiology. Copyright © American Society for Microbiology.en_HK
dc.rightsCopyright © American Society for Microbiology, Journal of Clinical Microbiology, 2002, v. 40 n. 5, p. 1675-1680en_HK
dc.subject.meshInfluenza A virus - isolation & purificationen_HK
dc.subject.meshInfluenza B virus - isolation & purificationen_HK
dc.subject.meshInfluenza, Human - classification - diagnosisen_HK
dc.subject.meshNasopharynx - virologyen_HK
dc.subject.meshReproducibility of Resultsen_HK
dc.titleEvaluation of the directigen FluA+B test for rapid diagnosis of influenza virus type A and B infectionsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0095-1137&volume=40&issue=5&spage=1675&epage=1680&date=2002&atitle=Evaluation+of+the+Directigen+FluA+B+test+for+rapid+diagnosis+of+influenza+virus+type+A+and+B+infectionsen_HK
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hken_HK
dc.identifier.authorityPeiris, JSM=rp00410en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1128/JCM.40.5.1675-1680.2002en_HK
dc.identifier.pmid11980941en_HK
dc.identifier.pmcidPMC130655en_HK
dc.identifier.scopuseid_2-s2.0-0036255946en_HK
dc.identifier.hkuros66960-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036255946&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume40en_HK
dc.identifier.issue5en_HK
dc.identifier.spage1675en_HK
dc.identifier.epage1680en_HK
dc.identifier.isiWOS:000175428200018-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChan, KH=35338760600en_HK
dc.identifier.scopusauthoridMaldeis, N=55348988300en_HK
dc.identifier.scopusauthoridPope, W=7005639325en_HK
dc.identifier.scopusauthoridYup, A=6504502458en_HK
dc.identifier.scopusauthoridOzinskas, A=6505895707en_HK
dc.identifier.scopusauthoridGill, J=7401975997en_HK
dc.identifier.scopusauthoridSeto, WH=7005799377en_HK
dc.identifier.scopusauthoridShortridge, KF=7005677034en_HK
dc.identifier.scopusauthoridPeiris, JSM=7005486823en_HK

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