File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Isolation and characterization of a Salmonella enterica serotype Typhi variant and its clinical and public health implications

TitleIsolation and characterization of a Salmonella enterica serotype Typhi variant and its clinical and public health implications
Authors
Issue Date2001
PublisherAmerican Society for Microbiology.
Citation
Journal Of Clinical Microbiology, 2001, v. 39 n. 3, p. 1190-1194 How to Cite?
AbstractWe report the isolation and characterization of a member of the family Enterobacteriaceae isolated from the gallbladder pus of a food handler. Conventional biochemical tests suggested Salmonella enterica serotype Typhi, but the isolate agglutinated with poly(O), 20, 90, and Vi Salmonella antisera but not with poly(H) or any individual H Salmonella antisera. 16S rRNA gene sequencing showed that there were two base differences between the isolate and Salmonella enterica serotype Montevideo, four base differences between the isolate and serotype Typhi, five base differences between the isolate and Salmonella enterica serotype Typhimurium, and six base differences between the isolate and Salmonella enterica serotype Dublin, indicating that the isolate was a strain of S. enterica. Electron microscopy confirmed that the isolate was aflagellated. The flagellin gene sequence of the isolate was 100% identical to that of the H1-d flagellin gene of serotype Typhi. Sequencing of the rfbE gene, which encoded the CDP-tyvelose epimerase of the isolate, showed that there was a point mutation at position +694 (G→T), leading to an amino acid substitution (Gly→Cys). This may have resulted in a protein of reduced catalytic activity and hence the presence of both 20 and 90 antigens. We therefore concluded that the isolate was a variant of serotype Typhi. Besides antibiotic therapy and cholecystectomy, removal of all stones in the biliary tree was performed for eradication of the carrier state.
Persistent Identifierhttp://hdl.handle.net/10722/49208
ISSN
2015 Impact Factor: 3.631
2015 SCImago Journal Rankings: 2.151
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWoo, PCYen_HK
dc.contributor.authorFung, AMYen_HK
dc.contributor.authorWong, SSYen_HK
dc.contributor.authorTsoi, HWen_HK
dc.contributor.authorYuen, KYen_HK
dc.date.accessioned2008-06-12T06:36:46Z-
dc.date.available2008-06-12T06:36:46Z-
dc.date.issued2001en_HK
dc.identifier.citationJournal Of Clinical Microbiology, 2001, v. 39 n. 3, p. 1190-1194en_HK
dc.identifier.issn0095-1137en_HK
dc.identifier.urihttp://hdl.handle.net/10722/49208-
dc.description.abstractWe report the isolation and characterization of a member of the family Enterobacteriaceae isolated from the gallbladder pus of a food handler. Conventional biochemical tests suggested Salmonella enterica serotype Typhi, but the isolate agglutinated with poly(O), 20, 90, and Vi Salmonella antisera but not with poly(H) or any individual H Salmonella antisera. 16S rRNA gene sequencing showed that there were two base differences between the isolate and Salmonella enterica serotype Montevideo, four base differences between the isolate and serotype Typhi, five base differences between the isolate and Salmonella enterica serotype Typhimurium, and six base differences between the isolate and Salmonella enterica serotype Dublin, indicating that the isolate was a strain of S. enterica. Electron microscopy confirmed that the isolate was aflagellated. The flagellin gene sequence of the isolate was 100% identical to that of the H1-d flagellin gene of serotype Typhi. Sequencing of the rfbE gene, which encoded the CDP-tyvelose epimerase of the isolate, showed that there was a point mutation at position +694 (G→T), leading to an amino acid substitution (Gly→Cys). This may have resulted in a protein of reduced catalytic activity and hence the presence of both 20 and 90 antigens. We therefore concluded that the isolate was a variant of serotype Typhi. Besides antibiotic therapy and cholecystectomy, removal of all stones in the biliary tree was performed for eradication of the carrier state.en_HK
dc.format.extent384 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherAmerican Society for Microbiology.en_HK
dc.relation.ispartofJournal of Clinical Microbiologyen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsJournal of Clinical Microbiology. Copyright © American Society for Microbiology.en_HK
dc.rightsCopyright © American Society for Microbiology, Journal of Clinical Microbiology, 2001, v. 39 n. 3, p. 1190-1194en_HK
dc.subject.meshCholangitis - microbiologyen_HK
dc.subject.meshCholecystitis - microbiologyen_HK
dc.subject.meshSalmonella typhi - classification - genetics - isolation & purificationen_HK
dc.subject.meshTyphoid Fever - microbiologyen_HK
dc.subject.meshTransaminases - geneticsen_HK
dc.titleIsolation and characterization of a Salmonella enterica serotype Typhi variant and its clinical and public health implicationsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0095-1137&volume=39&issue=3&spage=1190&epage=1194&date=2001&atitle=Isolation+and+characterization+of+a+Salmonella+enterica+serotype+Typhi+variant+and+its+clinical+and+public+health+implicationsen_HK
dc.identifier.emailWoo, PCY:pcywoo@hkucc.hku.hken_HK
dc.identifier.emailWong, SSY:samsonsy@hkucc.hku.hken_HK
dc.identifier.emailTsoi, HW:hwtsoi@hkucc.hku.hken_HK
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityWoo, PCY=rp00430en_HK
dc.identifier.authorityWong, SSY=rp00395en_HK
dc.identifier.authorityTsoi, HW=rp00439en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1128/JCM.39.3.1190-1194.2001en_HK
dc.identifier.pmid11230457-
dc.identifier.pmcidPMC87903en_HK
dc.identifier.scopuseid_2-s2.0-0035097598en_HK
dc.identifier.hkuros61930-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035097598&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume39en_HK
dc.identifier.issue3en_HK
dc.identifier.spage1190en_HK
dc.identifier.epage1194en_HK
dc.identifier.isiWOS:000167316900067-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridWoo, PCY=7201801340en_HK
dc.identifier.scopusauthoridFung, AMY=7101926801en_HK
dc.identifier.scopusauthoridWong, SSY=13310021400en_HK
dc.identifier.scopusauthoridTsoi, HW=6603822102en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats