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Article: Enhancement by ampicillin of antibody responses induced by a protein antigen and a DNA vaccine carried by live-attenuated Salmonella enterica serovar Typhi

TitleEnhancement by ampicillin of antibody responses induced by a protein antigen and a DNA vaccine carried by live-attenuated Salmonella enterica serovar Typhi
Authors
Issue Date2000
PublisherAmerican Society for Microbiology.
Citation
Clinical And Diagnostic Laboratory Immunology, 2000, v. 7 n. 4, p. 596-599 How to Cite?
AbstractLive-attenuated Salmonella species are effective carriers of microbial antigens and DNA vaccines. In a mouse model, the immunoglobulin M (IgM) and total antibody levels directed toward the lipopolysaccharide of Salmonella enterica serovar Typhi were significantly enhanced at day 21 after oral immunization with live-attenuated serovar Typhi (strain Ty21a) when ampicillin was concomitantly administered (P < 0.05 and P < 0.005, respectively). The heat-killed Ty21a-stimulated lymphocyte proliferation indices for the ampicillin group at day 21 were significantly higher than those for the normal saline (NS) group (P < 0.005, P < 0.001, and P < 0.01) for all three doses of antigen (10 4, 10 5, and 10 6 heat-killed Ty21a per well, respectively). The 50% lethal doses for mice from the ampicillin and NS groups immunized with Ty21a with pBR322 after wild-type serovar Typhi challenge on day 24 were 3.4 x 10 7 and 5.0 x 10 6 CFU, respectively. The fecal bacterial counts for the ampicillin group at days 1, 3, and 5 were significantly lower than those for the NS group (P < 0.01, P < 0.01, and P < 0.05, respectively), and there was a trend toward recovery of Ty21a in a larger number of mice from the ampicillin group than from the NS group. Furthermore, the IgG2a levels directed toward tetanus toxoid were significantly enhanced at days 7 and 21 after oral immunization with Ty21a that carried the fragment c of tetanus toxoid when ampicillin was concomitantly administered (P < 0.05 and P < 0.005, respectively), and the IgM and total hepatitis B surface antibody levels were significantly enhanced at days 7 (P < 0.005 and P < 0.05, respectively) and 21 (P < 0.01 and P < 0.05, respectively) after oral immunization with Ty21a that carried the DNA vaccine that encodes hepatitis B surface antigen when ampicillin was concomitantly administered. The present observation may improve the efficacy of the protein antigens and DNA vaccines carried in live-attenuated bacteria, and further experiments should be carried out to determine the best antibiotics and dosage regimen to be used, as well as the best carrier system for individual protein antigens and DNA vaccines.
Persistent Identifierhttp://hdl.handle.net/10722/49207
ISSN
2007 Impact Factor: 2.511
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWoo, PCYen_HK
dc.contributor.authorTsoi, HWen_HK
dc.contributor.authorLeung, HCHen_HK
dc.contributor.authorWong, LPen_HK
dc.contributor.authorChan, Een_HK
dc.contributor.authorYuen, KYen_HK
dc.date.accessioned2008-06-12T06:36:45Z-
dc.date.available2008-06-12T06:36:45Z-
dc.date.issued2000en_HK
dc.identifier.citationClinical And Diagnostic Laboratory Immunology, 2000, v. 7 n. 4, p. 596-599en_HK
dc.identifier.issn1071-412Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/49207-
dc.description.abstractLive-attenuated Salmonella species are effective carriers of microbial antigens and DNA vaccines. In a mouse model, the immunoglobulin M (IgM) and total antibody levels directed toward the lipopolysaccharide of Salmonella enterica serovar Typhi were significantly enhanced at day 21 after oral immunization with live-attenuated serovar Typhi (strain Ty21a) when ampicillin was concomitantly administered (P < 0.05 and P < 0.005, respectively). The heat-killed Ty21a-stimulated lymphocyte proliferation indices for the ampicillin group at day 21 were significantly higher than those for the normal saline (NS) group (P < 0.005, P < 0.001, and P < 0.01) for all three doses of antigen (10 4, 10 5, and 10 6 heat-killed Ty21a per well, respectively). The 50% lethal doses for mice from the ampicillin and NS groups immunized with Ty21a with pBR322 after wild-type serovar Typhi challenge on day 24 were 3.4 x 10 7 and 5.0 x 10 6 CFU, respectively. The fecal bacterial counts for the ampicillin group at days 1, 3, and 5 were significantly lower than those for the NS group (P < 0.01, P < 0.01, and P < 0.05, respectively), and there was a trend toward recovery of Ty21a in a larger number of mice from the ampicillin group than from the NS group. Furthermore, the IgG2a levels directed toward tetanus toxoid were significantly enhanced at days 7 and 21 after oral immunization with Ty21a that carried the fragment c of tetanus toxoid when ampicillin was concomitantly administered (P < 0.05 and P < 0.005, respectively), and the IgM and total hepatitis B surface antibody levels were significantly enhanced at days 7 (P < 0.005 and P < 0.05, respectively) and 21 (P < 0.01 and P < 0.05, respectively) after oral immunization with Ty21a that carried the DNA vaccine that encodes hepatitis B surface antigen when ampicillin was concomitantly administered. The present observation may improve the efficacy of the protein antigens and DNA vaccines carried in live-attenuated bacteria, and further experiments should be carried out to determine the best antibiotics and dosage regimen to be used, as well as the best carrier system for individual protein antigens and DNA vaccines.en_HK
dc.format.extent384 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherAmerican Society for Microbiology.en_HK
dc.relation.ispartofClinical and Diagnostic Laboratory Immunologyen_HK
dc.rightsClinical and Diagnostic Laboratory Immunology. Copyright © American Society for Microbiology.en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsCopyright © American Society for Microbiology, Clinical and Diagnostic Laboratory Immunology, 2000, v. 7 n. 4, p. 596-599en_HK
dc.subject.meshAmpicillin - pharmacologyen_HK
dc.subject.meshAntibodies, Bacterial - biosynthesis - immunologyen_HK
dc.subject.meshAntigens, Bacterial - immunologyen_HK
dc.subject.meshPenicillins - pharmacologyen_HK
dc.subject.meshSalmonella enterica - immunologyen_HK
dc.titleEnhancement by ampicillin of antibody responses induced by a protein antigen and a DNA vaccine carried by live-attenuated Salmonella enterica serovar Typhien_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1071-412X&volume=7&issue=4&spage=596&epage=599&date=2000&atitle=Enhancement+by+ampicillin+of+antibody+responses+induced+by+a+protein+antigen+and+a+DNA+vaccine+carried+by+live-attenuated+Salmonella+enterica+Serovar+typhien_HK
dc.identifier.emailWoo, PCY:pcywoo@hkucc.hku.hken_HK
dc.identifier.emailTsoi, HW:hwtsoi@hkucc.hku.hken_HK
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityWoo, PCY=rp00430en_HK
dc.identifier.authorityTsoi, HW=rp00439en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1128/CDLI.7.4.596-599.2000en_HK
dc.identifier.pmid10882658-
dc.identifier.pmcidPMC95920en_HK
dc.identifier.scopuseid_2-s2.0-0033939890en_HK
dc.identifier.hkuros61770-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033939890&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume7en_HK
dc.identifier.issue4en_HK
dc.identifier.spage596en_HK
dc.identifier.epage599en_HK
dc.identifier.isiWOS:000088051400014-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridWoo, PCY=7201801340en_HK
dc.identifier.scopusauthoridTsoi, HW=6603822102en_HK
dc.identifier.scopusauthoridLeung, HCH=36755846800en_HK
dc.identifier.scopusauthoridWong, LP=7402092221en_HK
dc.identifier.scopusauthoridChan, E=7401994013en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK

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