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Article: Identification of Mycobacterium neoaurum isolated from a neutropenic patient with catheter-related bacteremia by 16s rRNA sequencing

TitleIdentification of Mycobacterium neoaurum isolated from a neutropenic patient with catheter-related bacteremia by 16s rRNA sequencing
Authors
Issue Date2000
PublisherAmerican Society for Microbiology.
Citation
Journal Of Clinical Microbiology, 2000, v. 38 n. 9, p. 3515-3517 How to Cite?
AbstractA rapidly growing pigmented mycobacterial strain with an ambiguous biochemical profile was isolated from the blood culture taken through the Hickman catheter of a 9-year-old girl with acute lymphoblastic leukemia. Whole-cell fatty acid analysis showed that the best match profile was that of Mycobacterium aurum, but the similarity index was only 0.217, meaning that there were no good matches between the isolate and the organisms in the database of the Microbial Identification System. The 16S rRNA gene of the mycobacterial strain was amplified, agarose gel purified, and sequenced. There were 44 base differences between the gene sequence of the isolate and that of M. aurum but only one base difference between the sequence of the isolate and that of Mycobacterium neoaurum, showing that the isolate was indeed a strain of M. neoaurum by using this 'gold standard.' This represents the first case of M. neoaurum infection documented by 16S rRNA sequencing.
Persistent Identifierhttp://hdl.handle.net/10722/49206
ISSN
2015 Impact Factor: 3.631
2015 SCImago Journal Rankings: 2.151
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWoo, PCYen_HK
dc.contributor.authorTsoi, HWen_HK
dc.contributor.authorLeung, KWen_HK
dc.contributor.authorLum, PNLen_HK
dc.contributor.authorLeung, ASPen_HK
dc.contributor.authorMa, CHen_HK
dc.contributor.authorKam, KMen_HK
dc.contributor.authorYuen, KYen_HK
dc.date.accessioned2008-06-12T06:36:44Z-
dc.date.available2008-06-12T06:36:44Z-
dc.date.issued2000en_HK
dc.identifier.citationJournal Of Clinical Microbiology, 2000, v. 38 n. 9, p. 3515-3517en_HK
dc.identifier.issn0095-1137en_HK
dc.identifier.urihttp://hdl.handle.net/10722/49206-
dc.description.abstractA rapidly growing pigmented mycobacterial strain with an ambiguous biochemical profile was isolated from the blood culture taken through the Hickman catheter of a 9-year-old girl with acute lymphoblastic leukemia. Whole-cell fatty acid analysis showed that the best match profile was that of Mycobacterium aurum, but the similarity index was only 0.217, meaning that there were no good matches between the isolate and the organisms in the database of the Microbial Identification System. The 16S rRNA gene of the mycobacterial strain was amplified, agarose gel purified, and sequenced. There were 44 base differences between the gene sequence of the isolate and that of M. aurum but only one base difference between the sequence of the isolate and that of Mycobacterium neoaurum, showing that the isolate was indeed a strain of M. neoaurum by using this 'gold standard.' This represents the first case of M. neoaurum infection documented by 16S rRNA sequencing.en_HK
dc.format.extent384 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherAmerican Society for Microbiology.en_HK
dc.relation.ispartofJournal of Clinical Microbiologyen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsJournal of Clinical Microbiology. Copyright © American Society for Microbiology.en_HK
dc.rightsCopyright © American Society for Microbiology, Journal of Clinical Microbiology, 2000, v. 38 n. 9, p. 3515-3517en_HK
dc.subject.meshBacteremia - diagnosis - microbiologyen_HK
dc.subject.meshCatheterization, Central Venous - adverse effectsen_HK
dc.subject.meshMycobacterium - classification - genetics - isolation & purificationen_HK
dc.subject.meshMycobacterium Infections - diagnosis - microbiologyen_HK
dc.subject.meshNeutropenia - complicationsen_HK
dc.titleIdentification of Mycobacterium neoaurum isolated from a neutropenic patient with catheter-related bacteremia by 16s rRNA sequencingen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0095-1137&volume=38&issue=9&spage=3515&epage=3517&date=2000&atitle=Identification+of+Mycobacterium+neoaurum+isolated+from+a+neutropenic+patient+with+catheter-related+bacteremia+by+16S+rRNA+sequencingen_HK
dc.identifier.emailWoo, PCY:pcywoo@hkucc.hku.hken_HK
dc.identifier.emailTsoi, HW:hwtsoi@hkucc.hku.hken_HK
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityWoo, PCY=rp00430en_HK
dc.identifier.authorityTsoi, HW=rp00439en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.pmid10970421-
dc.identifier.pmcidPMC87424en_HK
dc.identifier.scopuseid_2-s2.0-0033831741en_HK
dc.identifier.hkuros61717-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033831741&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume38en_HK
dc.identifier.issue9en_HK
dc.identifier.spage3515en_HK
dc.identifier.epage3517en_HK
dc.identifier.isiWOS:000089114500075-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridWoo, PCY=7201801340en_HK
dc.identifier.scopusauthoridTsoi, HW=6603822102en_HK
dc.identifier.scopusauthoridLeung, KW=7401860831en_HK
dc.identifier.scopusauthoridLum, PNL=7005247001en_HK
dc.identifier.scopusauthoridLeung, ASP=7403012546en_HK
dc.identifier.scopusauthoridMa, CH=36955474600en_HK
dc.identifier.scopusauthoridKam, KM=36928498900en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK

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