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Article: groEL encodes a highly antigenic protein in Burkholderia pseudomallei

TitlegroEL encodes a highly antigenic protein in Burkholderia pseudomallei
Authors
Issue Date2001
PublisherAmerican Society for Microbiology.
Citation
Clinical And Diagnostic Laboratory Immunology, 2001, v. 8 n. 4, p. 832-836 How to Cite?
AbstractNo recombinant protein is available for serodiagnosis of melioidosis. In this study, we report the cloning of the groEL gene, which encodes an immunogenic protein of Burkholderia pseudomallei. Bidirectional DNA sequencing of groEL revealed that the gene contained a single open reading frame encoding 546 amino acid residues with a predicted molecular mass of 57.1 kDa. Basic Local Alignment Search Tool analysis showed that the putative protein encoded by groEL is homologous to the chaperonins encoded by the groEL genes of other bacteria. It has 98% amino acid identity with the GroEL of Burkholderia cepacia, 98% amino acid identity with the GroEL of Burkholderia vietnamiensis, and 82% amino acid identity with the GroEL of Bordetella pertussis. Furthermore, it was observed that patients with melioidosis develop a strong antibody response against GroEL, suggesting that the recombinant protein and its monoclonal antibody may be useful for serodiagnosis in patients with melioidosis and that the protein may represent a good cell surface target for host humoral immunity. Further studies in these directions would be warranted.
Persistent Identifierhttp://hdl.handle.net/10722/49204
ISSN
2007 Impact Factor: 2.511
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWoo, PCYen_HK
dc.contributor.authorLeung, PKLen_HK
dc.contributor.authorWong, SSYen_HK
dc.contributor.authorHo, PLen_HK
dc.contributor.authorYuen, KYen_HK
dc.date.accessioned2008-06-12T06:36:41Z-
dc.date.available2008-06-12T06:36:41Z-
dc.date.issued2001en_HK
dc.identifier.citationClinical And Diagnostic Laboratory Immunology, 2001, v. 8 n. 4, p. 832-836en_HK
dc.identifier.issn1071-412Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/49204-
dc.description.abstractNo recombinant protein is available for serodiagnosis of melioidosis. In this study, we report the cloning of the groEL gene, which encodes an immunogenic protein of Burkholderia pseudomallei. Bidirectional DNA sequencing of groEL revealed that the gene contained a single open reading frame encoding 546 amino acid residues with a predicted molecular mass of 57.1 kDa. Basic Local Alignment Search Tool analysis showed that the putative protein encoded by groEL is homologous to the chaperonins encoded by the groEL genes of other bacteria. It has 98% amino acid identity with the GroEL of Burkholderia cepacia, 98% amino acid identity with the GroEL of Burkholderia vietnamiensis, and 82% amino acid identity with the GroEL of Bordetella pertussis. Furthermore, it was observed that patients with melioidosis develop a strong antibody response against GroEL, suggesting that the recombinant protein and its monoclonal antibody may be useful for serodiagnosis in patients with melioidosis and that the protein may represent a good cell surface target for host humoral immunity. Further studies in these directions would be warranted.en_HK
dc.format.extent384 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherAmerican Society for Microbiology.en_HK
dc.relation.ispartofClinical and Diagnostic Laboratory Immunologyen_HK
dc.rightsClinical and Diagnostic Laboratory Immunology. Copyright © American Society for Microbiology.en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsCopyright © American Society for Microbiology, Clinical and Diagnostic Laboratory Immunology, 2001, v. 8 n. 4, p. 832-836en_HK
dc.subject.meshAntigens, Bacterial - genetics - immunologyen_HK
dc.subject.meshBurkholderia pseudomallei - classification - genetics - immunologyen_HK
dc.subject.meshGenes, Bacterialen_HK
dc.subject.meshAntibodies, Bacterial - blood - immunologyen_HK
dc.subject.meshBase Sequenceen_HK
dc.titlegroEL encodes a highly antigenic protein in Burkholderia pseudomalleien_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1071-412X&volume=8&issue=4&spage=832&epage=836&date=2001&atitle=groEL+encodes+a+highly+antigenic+protein+in+Burkholderia+pseudomalleien_HK
dc.identifier.emailWoo, PCY:pcywoo@hkucc.hku.hken_HK
dc.identifier.emailWong, SSY:samsonsy@hkucc.hku.hken_HK
dc.identifier.emailHo, PL:plho@hkucc.hku.hken_HK
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityWoo, PCY=rp00430en_HK
dc.identifier.authorityWong, SSY=rp00395en_HK
dc.identifier.authorityHo, PL=rp00406en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1128/CDLI.8.4.832-836.2001en_HK
dc.identifier.pmid11427437-
dc.identifier.pmcidPMC96153en_HK
dc.identifier.scopuseid_2-s2.0-0034964331en_HK
dc.identifier.hkuros61631-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034964331&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume8en_HK
dc.identifier.issue4en_HK
dc.identifier.spage832en_HK
dc.identifier.epage836en_HK
dc.identifier.isiWOS:000169705700029-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridWoo, PCY=7201801340en_HK
dc.identifier.scopusauthoridLeung, PKL=55085129100en_HK
dc.identifier.scopusauthoridWong, SSY=13310021400en_HK
dc.identifier.scopusauthoridHo, PL=7402211363en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK

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