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Article: Detection of RTX toxin gene in Vibrio cholerae by PCR

TitleDetection of RTX toxin gene in Vibrio cholerae by PCR
Authors
Issue Date2001
PublisherAmerican Society for Microbiology.
Citation
Journal Of Clinical Microbiology, 2001, v. 39 n. 7, p. 2594-2597 How to Cite?
AbstractA PCR that amplifies a recently discovered Vibrio cholerae RTX (repeat in toxin) toxin gene was developed. Among 166 clinical and environmental isolates of V. cholerae causing epidemics and sporadic cases of cholera in various parts of the world, all were found to be toxigenic by both PCR and HEp-2 cell cytotoxicity assay. Standard strains of the classical biotype containing a deletion within the gene cluster exhibited negative results by both assays. This is the first rapid genotyping method for differentiation of V. cholerae O1 classical biotype strains from El Tor biotype strains as well as strains of other non-O1 serogroups including serogroup O139. The PCR assay that was developed also specifically detects RTX toxin genes in V. cholerae, as clinical isolates of Vibrio parahaemolyticus, diarrheagenic Escherichia coli, Aeromonas species, and Plesiomonas species were all negative by the RTX toxin-specific PCR as well as the HEp-2 cytotoxicity assay. These findings highlight the characteristics of the RTX toxins in V. cholerae. Their role in the pathogenicity of the bacterium requires further investigation.
Persistent Identifierhttp://hdl.handle.net/10722/49203
ISSN
2015 Impact Factor: 3.631
2015 SCImago Journal Rankings: 2.151
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChow, KHen_HK
dc.contributor.authorNg, TKen_HK
dc.contributor.authorYuen, KYen_HK
dc.contributor.authorYam, WCen_HK
dc.date.accessioned2008-06-12T06:36:40Z-
dc.date.available2008-06-12T06:36:40Z-
dc.date.issued2001en_HK
dc.identifier.citationJournal Of Clinical Microbiology, 2001, v. 39 n. 7, p. 2594-2597en_HK
dc.identifier.issn0095-1137en_HK
dc.identifier.urihttp://hdl.handle.net/10722/49203-
dc.description.abstractA PCR that amplifies a recently discovered Vibrio cholerae RTX (repeat in toxin) toxin gene was developed. Among 166 clinical and environmental isolates of V. cholerae causing epidemics and sporadic cases of cholera in various parts of the world, all were found to be toxigenic by both PCR and HEp-2 cell cytotoxicity assay. Standard strains of the classical biotype containing a deletion within the gene cluster exhibited negative results by both assays. This is the first rapid genotyping method for differentiation of V. cholerae O1 classical biotype strains from El Tor biotype strains as well as strains of other non-O1 serogroups including serogroup O139. The PCR assay that was developed also specifically detects RTX toxin genes in V. cholerae, as clinical isolates of Vibrio parahaemolyticus, diarrheagenic Escherichia coli, Aeromonas species, and Plesiomonas species were all negative by the RTX toxin-specific PCR as well as the HEp-2 cytotoxicity assay. These findings highlight the characteristics of the RTX toxins in V. cholerae. Their role in the pathogenicity of the bacterium requires further investigation.en_HK
dc.format.extent384 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherAmerican Society for Microbiology.en_HK
dc.relation.ispartofJournal of Clinical Microbiologyen_HK
dc.rightsJournal of Clinical Microbiology. Copyright © American Society for Microbiology.en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsCopyright © American Society for Microbiology, Journal of Clinical Microbiology, 2001, v. 39 n. 7, p. 2594-2597en_HK
dc.subject.meshAcyltransferases - genetics - metabolism - toxicityen_HK
dc.subject.meshBacterial Proteinsen_HK
dc.subject.meshBacterial Toxins - genetics - metabolism - toxicityen_HK
dc.subject.meshPolymerase Chain Reaction - methodsen_HK
dc.subject.meshVibrio cholerae - genetics - metabolismen_HK
dc.titleDetection of RTX toxin gene in Vibrio cholerae by PCRen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0095-1137&volume=39&issue=7&spage=2594&epage=2597&date=2001&atitle=Detection+of+RTX+toxin+gene+in+Vibrio+cholerae+by+PCRen_HK
dc.identifier.emailChow, KH:khchowb@hku.hken_HK
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_HK
dc.identifier.emailYam, WC:wcyam@hkucc.hku.hken_HK
dc.identifier.authorityChow, KH=rp00370en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.identifier.authorityYam, WC=rp00313en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1128/JCM.39.7.2594-2597.2001en_HK
dc.identifier.pmid11427575-
dc.identifier.pmcidPMC88191en_HK
dc.identifier.scopuseid_2-s2.0-0034951688en_HK
dc.identifier.hkuros61174-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034951688&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume39en_HK
dc.identifier.issue7en_HK
dc.identifier.spage2594en_HK
dc.identifier.epage2597en_HK
dc.identifier.isiWOS:000169586400034-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChow, KH=7202180736en_HK
dc.identifier.scopusauthoridNg, TK=7402229817en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK
dc.identifier.scopusauthoridYam, WC=7004281720en_HK

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