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Article: Detection of cell wall mannoprotein Mp1p in culture supernatants of Penicillium marneffei and in sera of penicilliosis patients

TitleDetection of cell wall mannoprotein Mp1p in culture supernatants of Penicillium marneffei and in sera of penicilliosis patients
Authors
Issue Date1999
PublisherAmerican Society for Microbiology.
Citation
Journal Of Clinical Microbiology, 1999, v. 37 n. 4, p. 981-986 How to Cite?
AbstractMannoproteins are important and abundant structural components of fungal cell walls. The MP1 gene encodes a cell wall mannoprotein of the pathogenic fungus Penicillium marneffei. In the present study, we show that Mp1p is secreted into the cell culture supernatant at a level that can be detected by Western blotting. A sensitive enzyme-linked immunosorbent assay (ELISA) developed with antibodies against Mp1p was capable of detecting this protein from the cell culture supernatant of P. marneffei at 104 cells/ml. The anti- Mp1p antibody is specific since it fails to react with any protein-form lysates of Candida albicans, Histoplasma capsulatum, or Cryptococcus neoformans by Western blotting. In addition, this Mp1p antigen-based ELISA is also specific for P. marneffei since the cell culture supernatants of the other three fungi gave negative results. Finally, a clinical evaluation of sera from penicilliosis patients indicates that 17 of 26 (65%) patients are Mp1p antigen test positive. Furthermore, a Mp1p antibody test was performed with these serum specimens. The combined antibody and antigen tests for P. marneffei carry a sensitive of 88% (23 of 26), with a positive predictive value of 100% and a negative predictive value of 96%. The specificities of the tests are high since none of the 85 control sera was positive by either test.
Persistent Identifierhttp://hdl.handle.net/10722/49193
ISSN
2015 Impact Factor: 3.631
2015 SCImago Journal Rankings: 2.151
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorCao, Len_HK
dc.contributor.authorChan, KMen_HK
dc.contributor.authorChen, Den_HK
dc.contributor.authorVanittanakom, Nen_HK
dc.contributor.authorLee, Cen_HK
dc.contributor.authorChan, CMen_HK
dc.contributor.authorSirisanthana, Ten_HK
dc.contributor.authorTsang, DNCen_HK
dc.contributor.authorYuen, KYen_HK
dc.date.accessioned2008-06-12T06:36:28Z-
dc.date.available2008-06-12T06:36:28Z-
dc.date.issued1999en_HK
dc.identifier.citationJournal Of Clinical Microbiology, 1999, v. 37 n. 4, p. 981-986en_HK
dc.identifier.issn0095-1137en_HK
dc.identifier.urihttp://hdl.handle.net/10722/49193-
dc.description.abstractMannoproteins are important and abundant structural components of fungal cell walls. The MP1 gene encodes a cell wall mannoprotein of the pathogenic fungus Penicillium marneffei. In the present study, we show that Mp1p is secreted into the cell culture supernatant at a level that can be detected by Western blotting. A sensitive enzyme-linked immunosorbent assay (ELISA) developed with antibodies against Mp1p was capable of detecting this protein from the cell culture supernatant of P. marneffei at 104 cells/ml. The anti- Mp1p antibody is specific since it fails to react with any protein-form lysates of Candida albicans, Histoplasma capsulatum, or Cryptococcus neoformans by Western blotting. In addition, this Mp1p antigen-based ELISA is also specific for P. marneffei since the cell culture supernatants of the other three fungi gave negative results. Finally, a clinical evaluation of sera from penicilliosis patients indicates that 17 of 26 (65%) patients are Mp1p antigen test positive. Furthermore, a Mp1p antibody test was performed with these serum specimens. The combined antibody and antigen tests for P. marneffei carry a sensitive of 88% (23 of 26), with a positive predictive value of 100% and a negative predictive value of 96%. The specificities of the tests are high since none of the 85 control sera was positive by either test.en_HK
dc.format.extent384 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherAmerican Society for Microbiology.en_HK
dc.relation.ispartofJournal of Clinical Microbiologyen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsJournal of Clinical Microbiology. Copyright © American Society for Microbiology.en_HK
dc.rightsCopyright © American Society for Microbiology, Journal of Clinical Microbiology, 1999, v. 37 n. 4, p. 981-986en_HK
dc.subject.meshFungal Proteins - analysis - blood - geneticsen_HK
dc.subject.meshMembrane Glycoproteins - analysis - blood - geneticsen_HK
dc.subject.meshMycoses - blood - diagnosis - microbiologyen_HK
dc.subject.meshPenicillium - chemistry - genetics - immunologyen_HK
dc.subject.meshPredictive Value of Testsen_HK
dc.titleDetection of cell wall mannoprotein Mp1p in culture supernatants of Penicillium marneffei and in sera of penicilliosis patientsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0095-1137&volume=37&issue=4&spage=981&epage=986&date=1999&atitle=Detection+of+cell+wall+mannoprotein+Mp1p+in+culture+supernatants+of+Penicillium+marneffei+and+in+sera+of+penicilliosis+patientsen_HK
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.pmid10074513-
dc.identifier.pmcidPMC88636en_HK
dc.identifier.scopuseid_2-s2.0-0033066924en_HK
dc.identifier.hkuros45025-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033066924&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume37en_HK
dc.identifier.issue4en_HK
dc.identifier.spage981en_HK
dc.identifier.epage986en_HK
dc.identifier.isiWOS:000079105500018-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridCao, L=7401637818en_HK
dc.identifier.scopusauthoridChan, KM=26324790600en_HK
dc.identifier.scopusauthoridChen, D=15820196100en_HK
dc.identifier.scopusauthoridVanittanakom, N=6603939234en_HK
dc.identifier.scopusauthoridLee, C=7410162028en_HK
dc.identifier.scopusauthoridChan, CM=7404814453en_HK
dc.identifier.scopusauthoridSirisanthana, T=7004277229en_HK
dc.identifier.scopusauthoridTsang, DNC=7005609132en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK

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