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Article: MP1 encodes an abundant and highly antigenic cell wall mannoprotein in the pathogenic fungus Penicillium marneffei

TitleMP1 encodes an abundant and highly antigenic cell wall mannoprotein in the pathogenic fungus Penicillium marneffei
Authors
Issue Date1998
PublisherAmerican Society for Microbiology.
Citation
Infection And Immunity, 1998, v. 66 n. 3, p. 966-973 How to Cite?
AbstractWe cloned the MP1 gene, which encodes an abundant antigenic cell wall mannoprotein from the dimorphic pathogenic fungus Penicillium marneffei. MP1 is a unique gene without homologs in sequence databases. It codes for a protein, Mp1p, of 462 amino acid residues, with a few sequence features that are present in several cell wall proteins of Saccharomyces cerevisiae and Candida albicans. It contains two putative N glycosylation sites, a serine- and threonine-rich region for O glycosylation, a signal peptide, and a putative glycosylphosphatidylinositol attachment signal sequence. Specific anti-Mp1p antibody was generated with recombinant Mp1p protein purified from Escherichia coil to allow further characterization of Mp1p. Western blot analysis with anti-Mp1p antibody revealed that Mp1p has predominant bands with molecular masses of 58 and 90 kDa and that it belongs to a group of cell wall proteins that can be readily removed from yeast cell surfaces by glucanase digestion. In addition, Mp1p is an abundant yeast glycoprotein and has high affinity for concanavalin A, a characteristic indicative of a mannoprotein. Furthermore, ultrastructural analysis with immunogold staining indicated that Mp1p is present in the cell walls of the yeast, hyphae, and conidia of P. marneffei. Finally, it was observed that infected patients develop a specific antibody response against Mp1p, suggesting that this protein represents a good cell surface target for host humoral immunity.
Persistent Identifierhttp://hdl.handle.net/10722/49188
ISSN
2015 Impact Factor: 3.603
2015 SCImago Journal Rankings: 2.342
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorCao, Len_HK
dc.contributor.authorChan, CMen_HK
dc.contributor.authorLee, Cen_HK
dc.contributor.authorWong, SSYen_HK
dc.contributor.authorYuen, KYen_HK
dc.date.accessioned2008-06-12T06:36:21Z-
dc.date.available2008-06-12T06:36:21Z-
dc.date.issued1998en_HK
dc.identifier.citationInfection And Immunity, 1998, v. 66 n. 3, p. 966-973en_HK
dc.identifier.issn0019-9567en_HK
dc.identifier.urihttp://hdl.handle.net/10722/49188-
dc.description.abstractWe cloned the MP1 gene, which encodes an abundant antigenic cell wall mannoprotein from the dimorphic pathogenic fungus Penicillium marneffei. MP1 is a unique gene without homologs in sequence databases. It codes for a protein, Mp1p, of 462 amino acid residues, with a few sequence features that are present in several cell wall proteins of Saccharomyces cerevisiae and Candida albicans. It contains two putative N glycosylation sites, a serine- and threonine-rich region for O glycosylation, a signal peptide, and a putative glycosylphosphatidylinositol attachment signal sequence. Specific anti-Mp1p antibody was generated with recombinant Mp1p protein purified from Escherichia coil to allow further characterization of Mp1p. Western blot analysis with anti-Mp1p antibody revealed that Mp1p has predominant bands with molecular masses of 58 and 90 kDa and that it belongs to a group of cell wall proteins that can be readily removed from yeast cell surfaces by glucanase digestion. In addition, Mp1p is an abundant yeast glycoprotein and has high affinity for concanavalin A, a characteristic indicative of a mannoprotein. Furthermore, ultrastructural analysis with immunogold staining indicated that Mp1p is present in the cell walls of the yeast, hyphae, and conidia of P. marneffei. Finally, it was observed that infected patients develop a specific antibody response against Mp1p, suggesting that this protein represents a good cell surface target for host humoral immunity.en_HK
dc.format.extent418 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherAmerican Society for Microbiology.en_HK
dc.relation.ispartofInfection and Immunityen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsInfection and Immunity. Copyright © American Society for Microbiology.en_HK
dc.rightsCopyright © American Society for Microbiology, Infection and Immunity, 1998, v. 66 n. 3, p. 966-973en_HK
dc.subject.meshAntigens, Fungal - geneticsen_HK
dc.subject.meshFungal Proteins - geneticsen_HK
dc.subject.meshGenes, Fungalen_HK
dc.subject.meshMembrane Glycoproteins - geneticsen_HK
dc.subject.meshPenicillium - chemistry - genetics - immunologyen_HK
dc.titleMP1 encodes an abundant and highly antigenic cell wall mannoprotein in the pathogenic fungus Penicillium marneffeien_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0019-9567&volume=66&issue=3&spage=966&epage=973&date=1998&atitle=MP1+encodes+an+abundant+and+highly+antigenic+cell+wall+mannoprotein+in+the+pathogenic+fungus+Penicillium+marneffeien_HK
dc.identifier.emailWong, SSY:samsonsy@hkucc.hku.hken_HK
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityWong, SSY=rp00395en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.pmid9488383-
dc.identifier.pmcidPMC108003-
dc.identifier.scopuseid_2-s2.0-0031888737en_HK
dc.identifier.hkuros37904-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031888737&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume66en_HK
dc.identifier.issue3en_HK
dc.identifier.spage966en_HK
dc.identifier.epage973en_HK
dc.identifier.isiWOS:000072160900013-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridCao, L=7401637818en_HK
dc.identifier.scopusauthoridChan, CM=7404814453en_HK
dc.identifier.scopusauthoridLee, C=7410162028en_HK
dc.identifier.scopusauthoridWong, SSY=13310021400en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK

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