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Article: Comparison of a novel, inhibitor-potentiated disc-diffusion test with other methods for the detection of extended-spectrum β-lactamases in Escherichia coli and Klebsiella pneumoniae

TitleComparison of a novel, inhibitor-potentiated disc-diffusion test with other methods for the detection of extended-spectrum β-lactamases in Escherichia coli and Klebsiella pneumoniae
Authors
Issue Date1998
PublisherOxford University Press. The Journal's web site is located at http://jac.oxfordjournals.org/
Citation
Journal of Antimicrobial Chemotherapy, 1998, v. 42 n. 1, p. 49-54 How to Cite?
AbstractA novel, inhibitor-potentiated disc-diffusion test for detecting extended-spectrum β-lactamases (ESBLs) in bacteria was evaluated. This test uses the principle of augmentation (by ≤ 10 mm) of inhibition zones produced by ceftazidime, cefotaxime, ceftriaxone or aztreonam discs on Mueller-Hinton agar supplemented with clavulanate (4 mg/L). The test was initially compared with the double-disc synergy test, Kirby-Bauer disc-diffusion test and Etest ESBL screen with a panel of 45 reference strains with known resistance profiles. This panel consisted of 27 ESBL-positive Escherichia coli strains expressing 14 Bush group 2be enzymes and 18 other E. coli and Klebsiella pneumoniae strains (14 non-ESBL β-lactamase producers and four non-β-lactamase producers). The Kirby-Bauer disc-diffusion test was the least sensitive method: 11-44% of the ESBL-positive control strains were misclassified as susceptible to ceftazidime, cefotaxime, ceftriaxone or aztreonam when interpreted by National Committee for Clinical Laboratory Standards (NCCLS) criteria. The sensitivities of the inhibitor-potentiated disc-diffusion test, the double-disc synergy test (when discs were 25 or 30 mm apart) and the Etest ESBL screen (with a breakpoint of > 4-fold reduction in ceftazidime MIC in the presence of clavulanate) were 100%, 96% and 89-96%, respectively. The inhibitor-potentiated disc-diffusion test was further evaluated with 81 E. coli and K. pneumoniae clinical isolates, which were identified as putative ESBL-producers by the double-disc synergy test. For these isolates, the sensitivity of both the inhibitor-potentiated disc-diffusion test and the Etest ESBL screen was 100%. In conclusion, the inhibitor-potentiated disc-diffusion test is a sensitive, convenient and inexpensive method of screening for ESBLs in E. coli and K. pneumoniae isolates, with potential for incorporation into routine clinical laboratory service.
Persistent Identifierhttp://hdl.handle.net/10722/49187
ISSN
2015 Impact Factor: 4.919
2015 SCImago Journal Rankings: 2.157
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHo, PLen_HK
dc.contributor.authorChow, KHen_HK
dc.contributor.authorYuen, KYen_HK
dc.contributor.authorNg, WWSen_HK
dc.contributor.authorChau, PYen_HK
dc.date.accessioned2008-06-12T06:36:20Z-
dc.date.available2008-06-12T06:36:20Z-
dc.date.issued1998en_HK
dc.identifier.citationJournal of Antimicrobial Chemotherapy, 1998, v. 42 n. 1, p. 49-54en_HK
dc.identifier.issn0305-7453en_HK
dc.identifier.urihttp://hdl.handle.net/10722/49187-
dc.description.abstractA novel, inhibitor-potentiated disc-diffusion test for detecting extended-spectrum β-lactamases (ESBLs) in bacteria was evaluated. This test uses the principle of augmentation (by ≤ 10 mm) of inhibition zones produced by ceftazidime, cefotaxime, ceftriaxone or aztreonam discs on Mueller-Hinton agar supplemented with clavulanate (4 mg/L). The test was initially compared with the double-disc synergy test, Kirby-Bauer disc-diffusion test and Etest ESBL screen with a panel of 45 reference strains with known resistance profiles. This panel consisted of 27 ESBL-positive Escherichia coli strains expressing 14 Bush group 2be enzymes and 18 other E. coli and Klebsiella pneumoniae strains (14 non-ESBL β-lactamase producers and four non-β-lactamase producers). The Kirby-Bauer disc-diffusion test was the least sensitive method: 11-44% of the ESBL-positive control strains were misclassified as susceptible to ceftazidime, cefotaxime, ceftriaxone or aztreonam when interpreted by National Committee for Clinical Laboratory Standards (NCCLS) criteria. The sensitivities of the inhibitor-potentiated disc-diffusion test, the double-disc synergy test (when discs were 25 or 30 mm apart) and the Etest ESBL screen (with a breakpoint of > 4-fold reduction in ceftazidime MIC in the presence of clavulanate) were 100%, 96% and 89-96%, respectively. The inhibitor-potentiated disc-diffusion test was further evaluated with 81 E. coli and K. pneumoniae clinical isolates, which were identified as putative ESBL-producers by the double-disc synergy test. For these isolates, the sensitivity of both the inhibitor-potentiated disc-diffusion test and the Etest ESBL screen was 100%. In conclusion, the inhibitor-potentiated disc-diffusion test is a sensitive, convenient and inexpensive method of screening for ESBLs in E. coli and K. pneumoniae isolates, with potential for incorporation into routine clinical laboratory service.en_HK
dc.format.extent418 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherOxford University Press. The Journal's web site is located at http://jac.oxfordjournals.org/en_HK
dc.relation.ispartofJournal of Antimicrobial Chemotherapyen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsJournal of Antimicrobial Chemotherapy. Copyright © Oxford University Press.-
dc.subject.meshEscherichia coli - drug effects - enzymologyen_HK
dc.subject.meshKlebsiella pneumoniae - drug effects - enzymologyen_HK
dc.subject.meshbeta-Lactamases - analysis - antagonists & inhibitorsen_HK
dc.subject.meshAnti-Bacterial Agents - pharmacologyen_HK
dc.subject.meshEnzyme Inhibitors - pharmacologyen_HK
dc.titleComparison of a novel, inhibitor-potentiated disc-diffusion test with other methods for the detection of extended-spectrum β-lactamases in Escherichia coli and Klebsiella pneumoniaeen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0305-7453&volume=42&issue=1&spage=49&epage=54&date=1998&atitle=Comparison+of+a+novel,+inhibitor-potentiated+disc-diffusion+test+with+other+methods+for+the+detection+of+extended-spectrum+β-lactamases+in+Escherichia+coli+and+Klebsiella+pneumoniaeen_HK
dc.identifier.emailHo, PL: plho@hkucc.hku.hken_HK
dc.identifier.emailChow, KH: khchowb@hku.hken_HK
dc.identifier.emailYuen, KY: kyyuen@hkucc.hku.hken_HK
dc.identifier.emailNg, WWS: wilson@hkuspace.hku.hk-
dc.identifier.authorityHo, PL=rp00406en_HK
dc.identifier.authorityChow, KH=rp00370en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1093/jac/42.1.49en_HK
dc.identifier.pmid9700527-
dc.identifier.scopuseid_2-s2.0-0031816621en_HK
dc.identifier.hkuros37859-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031816621&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume42en_HK
dc.identifier.issue1en_HK
dc.identifier.spage49en_HK
dc.identifier.epage54en_HK
dc.identifier.isiWOS:000075013800005-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridHo, PL=7402211363en_HK
dc.identifier.scopusauthoridChow, KH=7202180736en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK
dc.identifier.scopusauthoridNg, WS=36787042500en_HK
dc.identifier.scopusauthoridChau, PY=36509704300en_HK

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